Qiagen QIAcuity 911000 User Manual page 231

Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured target of interest and reference targets are part of the same reaction

mix):
On the right side above the table there is checkbox which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 4 columns indicating
following mean values:
Mean concentration value

CI (95%) − CI of mean concentration as percentage

Mean copies/genome

CI (95%) − CI of mean mutation fraction as percentage

Simplex test (configured target of interest and reference targets are part of different reaction

mixes):
For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:
Mean concentration value

CI (95%) − CI of mean concentration as percentage

Mean copies/genome

CI (95%) − CI of mean mutation fraction as percentage

Note: If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the "Show mean values for
replicates" check box is marked and can't be changed. In this case there is a warning message
above the table and when the user hover on the checkbox, a tooltip appears to inform the user that
selected analysis source contains replicates of the reference sample and result is calculated only by
using mean results for replicates.
Note: When creating a report for this plate, the result list is automatically included in the report as
soon as at least one dedicated diagram for copy number variation (heatmap, point diagram,
Concentration diagram) is selected to be included. For more information on reports see section
Reports.
To export the results of the list view to an CSV file, click Export to CSV.
QIAcuity User Manual 06/2022 231