Qiagen QIAcuity 911000 User Manual page 221

1. Click the applicable wells in the plate layout. For more information, see Selecting wells for
analysis.
2. Select the applicable wild-type target from the Wild type target list.
3. Select the applicable edited target from the Edited target list.
4. To view the results of the analysis, click Show results.
5. The results are divided into several tabs. To view the contents of the tab, click the tab title.
List tab for genome editing
The List tab contains a table with an overview of the analyzed wells. The following columns are
available in the table:
Well ID – This column represents the well position in the plate layout.

Sample/NTC/Control – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control.
Reaction mix – This column contains the icon and the name of the well.

Target – This column shows all target names with its corresponding target type. Targets that

were selected as wild type or edit type are marked accordingly.
Concentration (copies/ul) – This column shows the concentration assigned to each target or

channel.
CI (95%) – This column shows the value of the confidence interval at a 95% confidence level.

Edited fraction – This column shows the edited fraction for the edited target as percentage.

CI (95%) – This column shows the value of the confidence interval for the edited target at a

95% confidence level.
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