Page 1 June 2022 QIAcuity ® User Manual 911000, 911020, 911040, 911050 QIAGEN GmbH QIAGEN Strasse 1, 40724 Hilden, GERMANY Sample to Insight__...
Page 2 QIAcuity User Manual 06/2022...
Page 3: Table Of Contents
Contents Introduction ........................7 About this user manual ..................... 7 Intended use of the QIAcuity ..................8 Requirements for QIAcuity users ................10 Safety Information ......................11 Proper use ......................11 Electrical safety ......................13 Environment......................14 Biological safety ....................14 Chemicals ......................15 Maintenance safety ....................16 Radiation safety .....................16 Symbols on the QIAcuity ..................17 General Description ......................18...
Page 4 Decontaminating the QIAcuity ................269 Regular maintenance procedure for QIAcuity Instrument Software ......269 Regular maintenance procedure for QIAcuity Software Suite ........271 Troubleshooting ......................272 General information .....................272 Contacting QIAGEN Technical Services ..............272 Performing a self-check on the QIAcuity instrument ...........272 QIAcuity User Manual 06/2022...
Page 5 Creating a support package with the QIAcuity instrument software ......273 Creating a support package with the QIAcuity Software Suite ........277 Troubleshooting the instrument and software ............279 Accessing the system status and clearing errors ............282 Glossary........................284 Appendix A – Technical Data ..................285 Operating conditions ....................285 Transport conditions .....................286 Storage conditions ....................286...
Page 6 Chemikalien ......................307 Wartungssicherheit ....................307 Strahlensicherheit ....................308 Symbole auf dem QIAcuity ..................308 Appendix E – User management permissions ..............309 Document Revision History ....................312 QIAcuity User Manual 06/2022...
Page 7: Introduction
Technical Services Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of QIAGEN products. If you have any questions or experience any difficulties regarding the QIAcuity or QIAGEN products in general, do not hesitate to contact us.
Page 8: Intended Use Of The Qiacuity
QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques.
Page 9 QIAcuity systems such as QIAcuity Nanoplates and QIAcuity PCR Reagents for the applications described in the kit handbooks. If QIAcuity is used with products other than QIAGEN kits or QIAGEN assays designed for dPCR, it is the user’s responsibility to validate the performance of such product combination for any particular application.
Page 10: Requirements For Qiacuity Users
Laboratory technicians or equivalent Appropriately trained or experienced personnel familiar with use of computers and automation in general Preventive maintenance QIAGEN service personnel or service Trained and authorized by QIAGEN technicians of an authorized agent Servicing QIAGEN service personnel or service...
Page 11: Safety Information
The QIAcuity must only be operated by qualified personnel who have been appropriately trained. Servicing of the QIAcuity must only be performed by a QIAGEN Field Service specialist. Perform the maintenance as described in the Maintenance Procedures section. QIAGEN charges for repairs that are required due to incorrect maintenance.
Page 12 Risk of explosion [W4] The QIAcuity is intended for use with reagents and substances supplied with QIAGEN kits or others that are outlined in respective Information for Use. Use of other reagents and substances may lead to fire or explosion. CAUTION...
Page 13: Electrical Safety
If liquid has spilled inside the instrument, power OFF the instrument, disconnect it from the  power outlet, and contact QIAGEN Technical Services. If the instrument becomes electrically unsafe, prevent other personnel from operating it, and contact QIAGEN Technical Services.
Page 14: Environment
The instrument may be electrically unsafe when: It or the line power cord appears to be damaged.  It has been stored under unfavorable conditions for a prolonged period.  It has been subjected to severe transport stresses.  Liquids come in contact directly with electrical components of the QIAcuity. ...
Page 15: Chemicals
Samples Samples may contain infectious agents. You should be aware of the health hazard presented by such agents and should use, store, and dispose of such samples according to the required safety regulations. WARNING Samples containing infectious agents [W9] Some samples used with this instrument may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations.
Page 16: Maintenance Safety
Maintenance safety WARNING/ Risk of personal injury and material damage [W11] CAUTION Only perform maintenance that is specifically described in this user manual. Risk of fire [W12] WARNING Do not allow cleaning fluid or decontamination agents to come into contact with the electrical parts of the QIAcuity.
Page 17: Symbols On The Qiacuity
Symbols on the QIAcuity Symbol Location Description Type plate on the back of the CE mark for European Conformity instrument Type plate at the back of the CSA listing mark for Canada and the instrument Type plate on the back of the RCM mark for Australia and New instrument Zealand...
Page 18: General Description
General Description The QIAcuity performs a fully automated processing of the QIAcuity Nanoplates, including all necessary steps of plate priming, sealing of partitions, thermocycling, and image analysis. Depending on the plate type, up to 8, 24, or 96 samples per plate can be analyzed. For high sensitivity applications, the QIAcuity Nanoplate 26K 8- or 24-well is available.
Page 19 Scheme of filling and partitioning of a well. Principle of priming and rolling to allow partitioning of the wells. Thermocycling: The second step and module is a high-accuracy plate thermocycler that performs the polymerase chain reaction. The cycling profile can be set in the QIAcuity Software Suite or the instrument software.
Page 20: External Features Of Qiacuity
Scheme of positive (green) and negative (blue) partitions after imaging. External features of QIAcuity Touchscreen The QIAcuity is controlled using a swivel-mounted touchscreen. To adjust the angle of the touchscreen, pull gently at the bottom edge. The touchscreen enables the user to see an overview of all plate slots and the corresponding process steps and remaining times.
Page 21 Important: We recommend using QIAGEN USB sticks only to ensure full compatibility. Important: Do not remove the USB stick while downloading or transferring data or software to or from the instrument.
Page 22 WARNING Electrical hazard [W17] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live and opening covers or removing parts is likely to expose live parts.
Page 23: Thermal Cycler
Back view of the QIAcuity. External hand scanner The QIAcuity Four and Eight instruments are equipped with a barcode scanner, which enables the user to scan the plate ID before loading. For QIAcuity One, a barcode scanner is available as accessory.
Page 24: Optical System
Optical system The optical system of the QIAcuity is a camera-based fluorescence microscopy system. The excitation source for the fluorescence dyes is a high-power white LED. This source in combination with a specific excitation filter is used to illuminate a whole well at a time. The fluorophores in the single partitions absorb that light and emit light that is being filtered by a detection filter, collected and imaged through an objective lens on a CMOS-camera chip (see image below for a detailed overview of the components).
Page 25: Installation Procedure
This section provides instructions on unpacking, packing, and installing the QIAcuity. The installation procedure of the product is recommended to be carried out by a certified QIAGEN Field Service Specialist. A person who is familiar with the laboratory and computer equipment should be present during the installation.
Page 26 Explosive atmosphere WARNING [W21] The QIAcuity is not designed for use in an explosive atmosphere. Risk of overheating CAUTION [C9] To ensure proper ventilation, maintain a minimum clearance of 10 cm at the sides and rear of the QIAcuity. Slits and openings that ensure the ventilation of the QIAcuity must not be covered.
Page 27: Installation Of Ac Power Cord
Electrical hazard WARNING [W8] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live and opening covers or removing parts is likely to expose live parts.
Page 28: Unpacking The Qiacuity
QIAGEN Technical Services. 8. Check that the QIAcuity is not damaged and that there are no loose parts. If anything is damaged, contact QIAGEN Technical Services. Make sure that the QIAcuity has equilibrated to ambient temperature before operating it. 9. Retain the package in case you need to transport the QIAcuity in the future. Refer to Packing the QIAcuity for more details.
Page 29: Installing The Qiacuity
Note: Before transporting the QIAcuity, the instrument must first be decontaminated. Refer to section Maintenance Procedures for more details. Then, prepare the instrument as follows: 1. Turn off the instrument and unplug the power cord. 2. Re-install the shipping fixation screw. 3.
Page 30 Removing the protective film from the QIAcuity touchscreen Carefully peel off the protective film from the QIAcuity touchscreen. Removing the shipping fixation screw Access the back of the instrument and remove the shipping fixation screw using a 4 mm hex wrench. Store the fixation screw on a safe place in case it is needed at a later point in time.
Page 31 If there is an initialization error, retry the initialization process by turning the instrument off and on again. If the problem persists, see Troubleshooting the instrument and software or contact QIAGEN Technical Services. Note: The instrument must be turned off at least once a week.
Page 32 Installing a fresh copy of the QIAcuity Software Suite Visit www.qiagen.com and go to the Software section of the QIAcuity product page to check if an updated software version is available for download. The QIAcuity Software Suite is designed to work with Windows 10 operating system.
Page 33 The QIAcuitySoftwareSuite.exe package needed for the installation of the QIAcuity Software Suite is provided on the USB drive that comes with the QIAcuity. Alternatively, you can download the QIAcuitySoftwareSuite.exe package from www.qiagen.com. Before starting the installation process using the QIAcuity Setup Wizard, please note the following important setup information: 1.
Page 34 To install the QIAcuity Software Suite on the notebook, follow the steps below. 1. Locate the QIAcuitySoftwareSuite.exe file and double-click it. The installation process starts. 2. Check the I accept the terms in the License Agreement box in the End-User License Agreement window and click Install.
Page 35 3. In the prompt window asking you if you allow changes to your device, click Yes. Note: During installation, some command line windows may appear in the foreground. Do not close any of them. QIAcuity User Manual 06/2022...
Page 36 4. When the installation is completed, click Close. 5. In the local host site that says the site is not secure, click Advanced. QIAcuity User Manual 06/2022...
Page 37 6. Click Continue to localhost on to the webpage. 7. You are directed to the QIAcuity Software Suite login screen. QIAcuity User Manual 06/2022...
Page 38: Upgrading Qiacuity Software Suite To New Version
Upgrading QIAcuity Software Suite to new version The latest Software Suite version 2.1 is only compatible with the control software version 2.1. If only one software component is updated, no connection between the Software Suite and the control software can be established. Important: It is strongly recommended to update the Software Suite first before proceeding with the control software.
Page 39 you will encounter an unidentified error – please refer to chapter: “Troubleshooting the instrument and software” for more details. Note: Backup will be done only in the upgrade phase, so no backup will be created when uninstalling, repairing, or installing a fresh Suite. The checkbox will be visible (in “Options” menu) also during the fresh installation, however it has no impact on the backup process.
Page 40 Updating QIAcuity Software Suite 1.2 or 2.0 To upgrade the QIAcuity Software Suite 1.2 or 2.0 to the newest version, follow the steps below. 1. Locate the QIAcuitySuite.exe file and double-click it. The installation process starts. Click Next. 2. Check the I accept the terms in the License Agreement box in the End-User License Agreement window and click Install.
Page 41 3. In the prompt window asking you if you allow changes to your device, click Yes. 4. The system will then automatically upgrade the existing installation. 5. When the installation is completed, click Finish. 6. Please note that the computer must be restarted after successful Suite upgrade. You will receive the following message: QIAcuity User Manual 06/2022...
Page 42 7. Click OK. 8. Then, click Restart and the computer will begin to restart. Updating QIAcuity Software Suite 1.1.3 to 1.2.18 This chapter describes the protocol for the update of the QIAcuity Software Suite 1.1.3 to 2.1 and Control Software 0.5.2.18 to 2.1. Note: The Software Suite 1.1.3 cannot be updated directly to version 2.1 but has to be updated to version 1.2.18 first.
Page 43 Note: Plates from the QIAcuity Software Suite 1.1.3 need to be updated together with the software suite update. Plates created with the QIAcuity Software Suite 1.1.3 cannot be imported into the QIAcuity Software Suite 1.2.18 or newer, separately. 2. Ensure that a direct connection or a connection via network is already established and that the instrument and the PC running the QIAcuity Software Suite 1.1.3 are connected.
Page 44 5. Please make sure that the Destination Folder points to the existing installation of the QIAcuity Software Suite. Click Next in the Destination Folder window. 6. Click Install to start the installation. QIAcuity User Manual 06/2022...
Page 45 7. In the prompt window asking if changes are allowed to the device, click Yes. The system will then automatically upgrade the existing installation. 8. When the installation is completed, click Finish. 9. Update QIAcuity Suite as outlined in section “Updating QIAcuity Software Suite 1.2 or 2.0”, on page 40.
Page 46: Uninstalling The Qiacuity Software Suite
Uninstalling the QIAcuity Software Suite To uninstall the QIAcuity Software Suite from your system, follow the steps below. Please note that this will lead to loss of all data including plate runs. 1. Go to the Control Panel app. QIAcuity User Manual 06/2022...
Page 47 2. Select Uninstall a program from the Programs menu. 3. Select QIAcuity Software Suite from the list, and click Uninstall. 4. In the prompt window asking you if you allow changes to your device, click Yes. QIAcuity User Manual 06/2022...
Page 48 5. In the uninstallation wizard, read the description for each action. Note: The uninstall process will result in database deletion; all plate data will be lost. 6. Click the Uninstall and delete button, and the QIAcuity Software Suite uninstallation process will start.
Page 49: Updating The Instrument Software
Note: After uninstalling the Software Suite, the User database remains on the computer prevailed. To remove it, go to C:\ProgramData\Qiagen and remove the \Qiaidentity directory manually. Be aware that \ProgramData might be hidden and you need to change the view settings of the explorer.
Page 50 Visit www.qiagen.com and go to the Software section of the QIAcuity product page to check if an updated software version is available for download. Note: Only users with an Administrator and Lab Leader role can perform software updates. On a computer running Microsoft Windows, download the software update from www.qiagen.com.
Page 51: Establishing A Connection Between The Qiacuity Instrument And The Qiacuity Software Suite
19a. If the Software Suite is not yet connected, login in with the SetupUser credentials. 19b. Check your Software Suite settings. If all settings are done properly, press Test Connection and Save. 19c. Once the Software Suite is detected, a pop-up will state that a new Software Suite was detected and a restart is needed.
Page 52 10Mbit/s for a setup via network Note: Please make sure that the QIAcuity instrument is connected to the LAN. Any other configurations are not supported by QIAGEN. Note: Only users with Administrator role can modify the network configuration. We recommend to consult your network administrator when configuring the network.
Page 53 Note: If you changed the connection with the instrument from direct to LAN or from LAN to direct, or the IP was reassigned, refer to point (d) under the Installation and maintenance section of Troubleshooting the instrument and software. Follow the steps below to configure the notebook running the QIAcuity Software Suite. 1.
Page 54 4. On the left pane, click Change adapter settings. 5. Right-click the ethernet network adapter and select Properties. 6. Select Internet Protocol Version 4 (TCP/IPv4) and click Properties. QIAcuity User Manual 06/2022...
Page 55 7. Select Obtain an IP address automatically. If your organization does not provide DNS details, select Obtain DNS server address automatically as well. Click OK, then Close. Optional: You can check if the addresses have been assigned properly by following the steps below.
Page 56 3. Select the Ethernet tab. 4. Check the DHCP Enabled box. If you check this box, the IP address and MAC address fields are disabled. The assigned IP and MAC addresses of device are displayed in the IP address and MAC address fields. 5.
Page 57 Configuring a direct cable connection between the QIAcuity instrument and the QIAcuity Software Suite Note: Before you start, ensure that the QIAcuity instrument and the notebook are connected with an Ethernet cable. Follow the steps below to configure the notebook running the QIAcuity Software Suite. 1.
Page 58 4. On the left pane, click Change adapter settings. 5. Right-click the ethernet network adapter and select the Properties option. 6. Select Internet Protocol Version 4 (TCP/IPv4) and click Properties: QIAcuity User Manual 06/2022...
Page 59 7. Select Use the following IP address. Enter the following information: IP address: Enter 192.168.1.1.  Subnet mask: Enter 255.255.255.0.  Default gateway: Enter 192.168.1.254.  Preferred DNS server: Enter the DNS server address.  Alternative DNS server: Enter the alternative DNS server address. ...
Page 60 Follow the steps below to configure the QIAcuity instrument. 1. On the toolbar, tap Configuration. 2. Select the Ethernet tab. 3. Ensure that the DHCP Enabled box is not checked. Enter the following information: IP address: Enter 192.168.1.2.  Subnet mask: Enter 255.255.255.0. ...
Page 61 Configuring the connection to the QIAcuity Software Suite in the QIAcuity instrument software The QIAcuity instrument needs to be connected to the QIAcuity Software Suite to enable the exchange of data. To establish a connection, the instrument and the device in which the QIAcuity Software Suite is running must be connected to the same network.
Page 62 5. Enter the IP address in the address field of the Software Suite URL section. 6. If required, enter the port number (default: 8687). Note: To get the IP address of the Suite Server, the instrument must be connected to the Suite Server.
Page 63 7. If you do not want to configure proxy settings, ensure that the Do not use a proxy box is checked. Then, tap Test to test the connection. Note: An error message displays if the address and port number you entered are invalid. Tap OK to close the error message, and then re-enter the correct address and port number in the address field in the Software Suite URL area.
Page 64 8. If you want to configure the proxy settings in your network configuration, clear the Do not use a proxy box and enter the following information: Proxy Server: Enter the proxy server address.  Proxy Port: Enter the port address. ...
Page 65: Getting Started With The Qiacuity
Getting started with the QIAcuity Configuration of the QIAcuity If you are using QIAcuity for the first time, we recommend that you define the required settings. Other settings can be changed later, when needed. For more information about using the touchscreen and software, refer to Operating the QIAcuity Instrument.
Page 66 7. The Home screen appears. Note: To return to the Home screen from another screen, tap Running Status. Setting basic system data This section describes how to set your preferred name for the instrument. To set your preferred QIAcuity name: 1.
Page 67 4. The default device name uses the following naming convention: QIAcuity-<serial number of the instrument>. Managing users The QIAcuity requires users to log in before accessing instrument functionalities. Each user must have a user account with an appropriate role assigned to it. The QIAcuity supports various pre-designed user roles and customer created user roles.
Page 68 QIAcuity User Manual 06/2022...
Page 69 Synchronizing labware with the QIAcuity Software Suite The QIAcuity Control Software and the QIAcuity Software Suite use labware files that include information about the nanoplate formats available. A labware file defines dimensional specification and default processing parameters for each Nanoplate. Information about the labware used by the QIAcuity can be downloaded from the Software Suite.
Page 70 4. The progress of the download is shown in the dialog box. Once the synchronization is complete, tap OK to finish the process. QIAcuity User Manual 06/2022...
Page 71: Operating The Qiacuity Instrument
Operating the QIAcuity Instrument The QIAcuity is operated through a touchscreen. Elements of the QIAcuity user interface are shown in the following table. Table 4. QIAcuity interface elements Button/Icon Function Starts the run Stops the run Starts runs on all loaded plates Stops all runs Closes an open tray Ejects selected tray...
Page 72 Entering text and numbers To enter text or numbers, tap the corresponding field. An on-screen keyboard is displayed on the touchscreen. In some cases, the value required in a text field must meet a specific criterion. If required, the criteria are specified in the corresponding input window.
Page 73 Note: For all text fields, a handheld scanner plugged into one of the USB-ports can be used to scan 1D barcodes. Buttons and icons related to the on-screen keyboards are shown in the following table. Table 5. On-screen keypad buttons and icons Button/Icon Function Removes one character to the left of the cursor...
Page 74 5. Note: When connection to the Software Suite hasn’t been established yet, log into the Instrument using the following credentials: Login: SetupUser Password: 2#ConnectSuite For further information, please refer to section Configuring the connection to the QIAcuity Software Suite in the QIAcuity instrument software. 6.
Page 75 2. Place a plate in one of the slots of the ejected tray. Ensure that the plate is placed in the correct orientation, facing the barcode toward the instrument and the QIAGEN lettering toward you. Also, ensure that the plate seal of the plate is intact and not overlapping to the sides for more than 1 mm.
Page 76 3. Tap Close Tray or press the physical button on the instrument to close the tray. 4. The instrument scans the barcodes on the plates. The instrument detects the availability of the plate and the label of the corresponding pane changes to Plate is detected. If the barcode matches to an existing experiment in the Software Suite, the loaded plate pane displays the defined run setup and can be started.
Page 77 6. When all plates are correctly labelled and the corresponding data is received from the QIAcuity Software Suite, start the run. To start the run on all plates simultaneously without making any changes, tap Run all.  To start the run of an individual plate without making any changes, tap the Run ...
Page 78 Note: The Edit plate button becomes available when a plate is loaded and the instrument successfully received the data from the Software Suite. The Create a new plate button is available when the plate’s barcode is not found in the Software Suite database or when the QIAcuity cannot connect to the Software Suite.
Page 79 Note: If you are creating a plate, you are automatically assigned as an owner of a plate. Owners are displayed under plate name on running status page. Modifying owners of the plate is only possible by editing the plate in Software Suite. 2.
Page 80 5. If you want to modify the temperature steps, refer to these steps: To edit or delete a temperature step, tap the More icon, then tap Edit or Delete.  To group the temperature steps, check the corresponding boxes of more than one ...
Page 81 7. Start the run in the Running Status window: To start the run on all plates simultaneously without making any changes, tap Run all.  To start the run of an individual plate without making any changes, tap the corresponding ...
Page 82 Linking a plate to a pre-defined plate without existing barcode If the instrument cannot match the barcode on a loaded plate to a barcode that already exists in the Software Suite, you can link the plate manually. Alternatively, you can create a new plate by following the steps in section Configuring a plate and starting a run.
Page 83 Tracking the run status Once the run has started, you can track the status of the run. The plate that the QIAcuity is currently working with is distinguishable through the following elements: The Running status is displayed in the pane. ...
Page 84 To view information about the individual steps of the run, tap dPCR Parameter, then tap the step which details you want to view. The instrument shows the status of each step of the run, and the remaining time of the current step. You can also view the parameters defined for each step. Table 6 shows the meaning of each status icon that are shown in the dPCR parameter step.
Page 85 When the run is finished, the status of the run changes to Done and the Eject button becomes available. To view the details about the run, tap Show details. To eject the plate, tap Eject button. Continuous loading and unloading of plates Note: The Continuous loading and unloading of plates function is only available with QIAcuity Eight and QIAcuity Four instruments.
Page 86 Note: Depending on the time frame for unloading/loading of plates, the drawer opening might be delayed some time to finalize current movement steps. Aborting a run If needed, a run can be stopped at any time. You can either abort all running plates or only ...
Page 87 Note: To modify a plate that was already used, you must remove it from the instrument. This ensures that the plate is unlocked and ready for modifications in the QIAcuity Software suite. If modifications should be done using the plate configurator, load the Plate again. Editing the run schedule Note: Editing the run schedule is only possible on QIAcuity Eight and QIAcuity Four and for those users having the appropriate permission (please see Table 9).
Page 88 To edit the run schedule, follow these steps: Note: Only runs that are not started yet (with Pending Run status) can be rearranged. 1. On the Running Status screen, tap Edit run schedule. 2. Tap the row corresponding to the plate that you want to move. 3.
Page 89 To view a list of all notifications and possible solutions to the errors, tap the Alarm icon. The last three errors are shown. If there are more than three errors, tap View all to view the full list of errors. Disk Monitoring The Disk Monitor icon located in the header shows the real-time usage of the disk (free space and usage percentage).
Page 90 2. Yellow - when the usage of the disk occupation rises above 75% 3. Red - when the remaining free disk space is less than 4 GB (~14%) In case of 2 and 3, additional information is shown to inform about the actions that should be taken to gain the disk space: forcing the clearing of images, which haven’t been transferred to Software Suite or setting up a connection with Software Suite.
Page 91 Logging out Note: If a run is processing, you can still access its status, even if you log out of the instrument. For more information, refer to Accessing the run status when you are logged out. To log out of the instrument, follow these steps: 1.
Page 92 Accessing the run status when you are logged out After you log out, the Login screen is displayed on the QIAcuity display. To view the run status of an ongoing run, tap Running status. The Running status screen is displayed in view-only mode. All functions are disabled.
Page 93: Operating The Qiacuity Software Suite
Operating the QIAcuity Software Suite Getting started This section describes the workspace within the QIAcuity Software Suite, its basic concepts, and the general software use. Note: If the network connection was set up, the Suite can be accessed via another laptop using the IP address of the Suite server.
Page 94 If you want to perform an experiment multiple times, you can create templates to enable a faster setup of plates. See section Setting up an experiment and section Managing your templates to get more information. To operate the QIAcuity Software Suite, a free disk space is needed. See Regular maintenance procedure of QIAcuity for more information.
Page 95: Concepts Of The Qiacuity Software Suite
The Logged-in user is shown in the main toolbar. For more information, see Concepts of the QIAcuity Software Suite. Information bar The information bar located at the bottom of the screen displays the software version. Concepts of the QIAcuity Software Suite User management The QIAcuity Software Suite requires users to log in before accessing instrument functionalities.
Page 96 Important: For update starting from Software Suite 2.0 and instrument control software 2.0, users will be migrated and forced to change their password during first login attempt (except the initial administrator user with login name “admin” provided by QIAGEN during the initial installation). When working with Good Manufacturing Practice (GMP)/Good Laboratory (GLP) regulations, please deactivate this initial admin user (for which the password does not expire) once at least an additional user with administrator rights has been created.
Page 97 Admin, Supervisor, Group leader, Technician, and Quality Assurance are predefined roles designed for GMP/GLP labs, whereas Admin, Lab leader, and Operator are designed for MBA labs. Note: All predefined roles cannot be edited or deleted. 1. Admin The administrator is the role outside of the laboratory being responsible to configure the system and provide individual user access and rights.
Page 98 The lab leader is the role inside the laboratory and is designed for labs performing MBA. Users with this role will have extensive access to all QIAcuity instrument control software and QIAcuity Software Suite functionalities required to process plates and analyze results. This role also allows for basic user management functionalities to read user descriptions and their permissions.
Page 99 In the table below, permissions assigned to the roles are presented. Table 9. Default role permissions Adminis- Supervisor Group Technician Quality Operat trator leader Assurance leader Log in Instrument Suite Software Instrument accesses Instrument Maintenance Experiment Schedule Create Support Package Plates Create Plate All Plates...
Page 100 Table continued from previous page Table 9. (cont.) Default role permissions Administrator Supervisor Group Technician Quality Operator leader Assurance leader Unlock Plate Set Plate Ownership Upload VPF Upgrade Plate Create Support Package Create Report for Analysis Sign Report Delete Report Templates Create Template Edit Template...
Page 101 Table continued from previous page Table 9. (cont.) Default role permissions Adminis-trator Supervisor Group Technician Quality Operator leader Assurance leader System configuration Registered Instruments Manage Archive Audit Trail configuration View Audit Trail Audit Trail Toggle X – role has access to this permission This section describes how the functionalities in the application are impacted by permission, which are assigned to user roles.
Page 102 3. Create Support Package — User can download a support package to a USB drive and upload a support package. Plates (Instrument and PC software) Create Plate — User can set up dPCR parameters (priming, cycling, imaging), reaction mixes (reagents), samples (control, non-control), and create Plate layout. Create a new Plate (fill out General Data and scan barcode) ...
Page 103 2. Edit Plate Data — User can check and edit parameters of owned Plate (dPCR parameters, plate layout (samples, reaction mixes (reagents), controls), and mark it as primed. Note: ‘Read owned Plate’ required to have fully available permission. 3. Edit Analysis Data — User can change the threshold and use lasso selection on the Analysis page of the owned Plates to verify the accuracy of the results.
Page 104 Plate ownership field in plate general data is active  5. Upload VPF — User can upload Volume Precision Factor. Upload VPF  The upload VPF button is visible in Plates overview.  The upload VPF button is visible in plate details. ...
Page 105 Templates (Instrument and PC software) If the user has any of the rights regarding templates, then the following requirements apply. The user can go to Template (top-bar menu). If the user does not have “Read templates” permissions, then a modal is presented as follows: “In order to view templates, you need to have ‘Read templates’...
Page 106 Archive (PC software) 1. Plate Archiving — User can archive the Plate. Note: ‘Read owned Plate’ or ‘Read all Plate’ required to have fully available permission. Go to Plates (top-bar menu)  If the user does not have “Read plates” permissions, then a modal is presented: ...
Page 107 Delete the plate from archive  The delete is visible button after clicking the three dots (...) on the plate.  The delete is visible in plate details.  User Management (PC software) 1. Read Users and Roles — User can see the list of users and the list of roles in the system. Go to the configuration panel ...
Page 108 See users list (assigned to role)  Users list button in row with role  Note: Roles designed for GMP/GLP (Administrator, Operator, Lab leader, Technician, Group Leader, Supervisor, Quality Assurance) cannot be edited. 3. Activate and Deactivate User — User can activate and deactivate a user. Note: ‘Read users and roles’...
Page 109 System configuration (PC software) 1. Registered Instrument — User can see list of registered instruments. Go to the configuration panel  Go to the Instrument names tab  See the list of registered instruments  2. Manage Archive — User can edit Archive location, detach Archive, turn on/off, and configure automatic archiving.
Page 110 New user creation All activities related to creating/editing/deactivating users can be found within the User Management tab in Users view. Note: To read users list, permission “Read users and roles” is required. To create or edit users, additionally permission “Create and Edit Users and Roles” is required. 1.
Page 111 3. Please remember that you can enter user login only once, and after user creation, it cannot be changed. To create a unique login, please use letters, numbers, and symbols. Minimal number of characters for login is 5. 4. When all information is filled out properly, click Next or Permissions to move to the next step. QIAcuity User Manual 06/2022...
Page 112 5. In the Permissions (second) step, you need to choose one role that will be assigned to the user. The roles’ descriptions can be found below. For detailed information about roles and permissions, you can check in the attached tables below the instruction. 6.
Page 113 7. Read the roles’ descriptions and choose the user role. 8. To see the permissions list assigned to each role, please click the show all permissions button below the role’s description. QIAcuity User Manual 06/2022...
Page 114 9. If you want to hide all descriptions, please click the hide all descriptions button. 10. If you want to see the description of the permissions related to each permissions group, please click the show all descriptions button. 11. If you want to hide the description of the permissions related to each permissions group, please click the Hide all descriptions button.
Page 115 If you want to go back and check or change user information, please click the Back to User Information button or the User information step in the stepper. 13. Click the Save button to confirm and create the user. QIAcuity User Manual 06/2022...
Page 116 It is possible to start with the user creation process from the Permissions step, but entries in the User information step mandatory will highlighted software (see screenshot hereafter). 1. As you can see in the picture hereafter, when you directly go to the second step, the icon from the first step, User information, changed and informs you that you missed the first step.
Page 117 3. If all inputs fields are filled out, click the Next button. 4. To create the user, please click the Save button. When you are in the user creation panel, at every moment, you can go back to the user list (by clicking the Back to Users List button), but please remember that all entered data will be lost.
Page 118 Editing an existing user To edit user data, please follow the below instructions: 1. On the users list, please find the user that you want to edit. 2. Click the Edit user icon. 3. At the left-hand side are three tabs related to editing user personal data and permissions. QIAcuity User Manual 06/2022...
Page 119 User information tab In this tab, you can change the user name and surname, as well as check the user’s login. To edit a user name or surname, please click on the input field and type in the new name/surname. User permissions tab In the User permissions tab, you can change the role and check the permissions description according to each role.
Page 120 User password tab In the User password tab, you can change the user’s password. On the “New password” field, you need to enter the new password. On the “Confirm password”, you need to re-enter the same password as in the “New password” field. Those passwords should be the same.
Page 121 User deactivation Users with Administrator role are able to deactivate other users to restrict access to the system for such users. Note: It is not possible to delete users. 1. Find the user you want to deactivate. 2. At the end of the row, please find the deactivate user icon and click it. 3.
Page 122 4. Please remember that after the user is deactivated, all Plates assigned to this user will not use the Owner but show them in plate owner as deactivated. If you activate the user again, Plate Ownership will be reassigned. 5. Click the Yes button to deactivate the user. User activation Deactivated users may still be activated by users with “Activate and Deactivate User”...
Page 123 The Users list table shows all deactivated users. 3. Please find the user you want to activate. 4. Click the activate user icon. 5. Read the text in the pop-up window. If you are sure you want to activate the user, click the Activate button;...
Page 124 New role creation All activities related to creating/editing/deleting roles and list of existing roles can be found within the User Management tab in Roles view. Note: To read roles list, permission “Read users and roles” is required. To create or edit users, additional permission “Create and Edit Users and Roles”...
Page 125 If you want to go back and check or change role name, please click the Back to Role name button or the Role name step in the stepper. 6. Click the Save button to confirm and create the role. Note: All entries need to be completed under Role name before moving to the Permission step. The software will indicate any missing information in the Role name step.
Page 126 Pressing the Users list button will bring you to the list of all users and their status. QIAcuity User Manual 06/2022...
Page 127 Editing existing role Note to edit role, you need “Create and Edit Roles” permission. Only roles created by User can be edited. Predefined roles cannot be edited. To edit role, please follow the below instructions: 1. On the roles list, please find the role that you want to edit. 2.
Page 128 3. At the left-hand side are two tabs related to role information and role permissions. Role information tab In this tab, you can change the role name and description. To edit a user name or surname, please click on the input field and type the new value. Role permissions tab In the Role permissions tab, you can change permissions assigned to role.
Page 129 In case when some users have been assigned to role you are modifying after pressing Save button, you will be presented with window informing how many users will be impacted and you can check impacted users list using drop-down list. To confirm your changes click Yes, edit role button.
Page 130 In case when no users have been assigned to role you are modifying, confirmation window is not presented. Role deletion Note to delete role you need “Delete role” permission. Predefined roles cannot be deleted. Only roles created by User with no users assigned can be deleted. To delete role, please follow the instructions below: 1.
Page 131 Plate Ownership Plate Ownership allows users to assign one or more users to a specific Plate. A user who creates a new Plate, imports a plate, or restores a plate from the Archive is assigned automatically as an owner of the Plate. Note: For imported or restored plates, the operating user will automatically be added as plate owner if no plate owner is already assigned to the plate or if the assigned plate owner is a deactivated owner.
Page 132 4. Click on the “Plate Ownership” input field. 5. Start writing the user name/surname/login. 6. Choose the user from drop-down list by clicking the chosen name. 7. User has been added. 8. If you want to add another user, start writing the user name/surname/login. Then, choose the user from the drop-down list.
Page 133 Removing an owner of the Plate 1. If you want to remove one owner of a Plate, click the close icon placed within the user name tag. 2. If you want to remove all owners, click the clear icon on the end of the input field. Log-in screen The main toolbar shows which user is currently logged-in.
Page 134: Audit Trail
2. Enter the current password in the Current password field and the new password in the New password field. In the Confirm password field, enter the same password you specified in the New password field. Click Save to change the password. 3.
Page 135 the tracker is turned OFF. In GMP environments, this toggle must not be set to OFF to maintain 21 CFR Part 11 compliance support. When switched on, the Audit Trail records all actions that happened in the system. Each record consists of the following: time stamps (day, month, year, hour) ...
Page 136 As shown below, the Audit trail tracker is turned off when the circle is at the left-hand side, the toggle is gray, and an “OFF” label appears at the right-hand side. To turn the tracker ON, click on the toggle. When the Audit trail tracker is turned on, the circle is at the right-hand side, the toggle is blue, and an “ON”...
Page 137 Events List Events list table The events list shows all events (logs) that happened in the system. There is no possibility to remove an event from the system by the User or Administrator. To display the Events list, on the main toolbar, click the Configuration tab. At the left-hand side, you can see the tabs menu.
Page 138 To find a specific event, you can scroll the list down or use the following filters. Search Search for Instrument ID, affected Plate ID, and user login You can search for Instrument ID number, affected Plate ID, or affected user login. Click on the search field: “Search for Instrument ID, affected Plate ID, user login”.
Page 139 Filters Filter by specific date or specific time frame If you want to find all events that happened in one specific day or time period, you need to use the filters “Start date” and “End date”. Click on the “Start date” input field or click the calendar icon. Set the date that are you interested in from the calendar.
Page 140 Then specify “End date” in similar manner. Note: You can select the same day as in “Start date” to filter only a single, specific day. Afterwards, the records on events list will be updated automatically. Filter: Category You need to use the “Category” filter to check all data related to one specific category (Instrument, Software Suite, or Plate).
Page 141 Filter: Event type Event type refers to the action that happened in the system on the Instrument, Suite (PC software), or Plate. If you don’t choose any option from the category, the filter displays a list of all event types (a sum of events type from all the three categories).
Page 142 Afterwards, the records list will be updated automatically. Filter: Initiated by The filter “Initiated by” is related to a person (search for user login) that performed actions in the system. Login is unique and cannot be duplicated, so filtering for it will provide the user specific results.
Page 143 Exporting to PDF All event details can be exported to a PDF file. 1. Click on the Audit trail Events list tab. 2. Apply filters to select for those events that should be exported. 3. At the right-hand side, above the Event list, you can find the Export to PDF option. Click on it. 4.
Page 144 Table 10. Shortcut functions in QIAcuity Software Suite Shortcut Function Copy Ctrl + C Paste Ctrl + V Navigate from one field to another Tab and arrow keys Displaying errors, warnings, and additional information The QIAcuity Software Suite displays errors, warnings, and additional information messages throughout the experiment to prompt the user to perform a required action.
Page 145: Setting Up An Experiment
Warning message A warning message is displayed in yellow in case further optimization of the experimental setup is recommended. A user interaction is not required to proceed with this step. Information message At certain fields in the experiment, you can view additional information regarding the required data to be entered on a field.
Page 146 2. Click New Plate. 3. Refer to Table 12 to provide the following required information in each tab: QIAcuity User Manual 06/2022...
Page 147 Table 12. Required steps in creating a plate Steps General data Enter the basic information about the plate:  Plate name  Plate type  Description  Labels  Plate ownership  Barcode dPCR parameters Specify the following information:  Priming profile ...
Page 148: Defining General Data
Table continued from previous page Table 12 (cont). Required steps in creating a plate Steps Plate layout Provide the following information:  Add reaction mix  Mark as blank  Add sample  Add control  Add NTC Defining General Data In the General Data tab, you can specify the basic information about the plate.
Page 149 Table 13. General data tab Field Field Help Plate name Specify the name of the plate. Plate type Select the plate type from the list. You can select Nanoplate 26K 8-well, Nanoplate 26K 24-well, Nanoplate 8.5K 24-well, or Nanoplate 8.5K 96-well. Description Enter a descriptive information about your experiment.
Page 150 Cycling tab You can define the temperature profile of your experiment in the Cycling tab. To do this, follow these steps: 1. In the Temperature field, specify the temperature of the step, then specify the duration of the temperature step in the Duration field. 2.
Page 151 The Imaging tab enables you to set the respective exposure duration and gain value for each channel. The channel options depend on the connected instrument. QIAGEN offers QIAcuity One in 2-plex and 5-plex version. QIAcuity Four and QIAcuity Eight offer 5-plex analysis. The following table shows the available channels provided in each instrument.
Page 152 The following actions can be performed when your run is finished: To reimage the plate with different settings, click +, then select Imaging.  To rerun the plate with additional cycles, click +, then select Cycling + Imaging  Important: After a run is finished, remove the plate from the instrument before adding a new imaging or cycling + imaging step.
Page 153 The Detailed List view tab displays the detailed information about the reaction mixes stored in your database. To create a new reaction mix: 1. Click New Reaction Mix. 2. The New Reaction Mix window appears. QIAcuity User Manual 06/2022...
Page 154 3. Enter the name of the reaction mix in the Reaction mix name field. The name must be unique and must not contain any special characters. You can enter up to 100 characters in this field. 4. Click Edit reaction mix color to select a color for the reaction mix. 5.
Page 155 3. Enter the following information in the required fields: Sample Name: Enter the sample name. The name must be unique and must not contain any  special characters. You can enter up to 100 characters in this field. Note: The Sample ID is automatically generated. This ID is unique for each sample created. Labels: You can add labels to your samples to help you categorize your experiment.
Page 156 3. Click Add Control. 4. The control is added to your database and can be used for your experiment. To add Non Template Controls: 1. Click the Non Template Controls tab. 2. Specify the name of your non template control in the Non Template Control Name field. 3.
Page 157 Defining plate layouts To define the plate layout, you must add reaction mix, samples, and controls to your plate. Adding a reaction mix to the plate 1. Left-click the well you want to mark. 2. Left-click the + icon, then select Add reaction mix or Mark as blank. The Add reaction mix window appears.
Page 158 4. Click Assign to add the reaction mix to the well. Note: To edit or remove the reaction mix from the well, right-click the well and select Edit reaction mix or Remove reaction mix. You can also remove the reaction mix from the well by clicking the icon and select Remove reaction mix.
Page 159 Adding controls to the plate 1. On a marked well, click the icon or right-click the well. Note: You can also mark more than one well if you want to assign a control to multiple wells. 2. Click Add control. 3.
Page 160 Adding NTCs to the plate 1. On a marked well, left-click the icon or right-click the well. Note: You can also mark more than one well if you want to assign a Non Template Control to multiple wells. 2. Click Add NTC. 3.
Page 161 List view The list view in the Plate Layout tab displays a detailed overview about your plate setup. You can modify this view by sorting and filtering: Sort by rows or columns  Filter by existing reaction mixes  Hide specific columns ...
Page 162 The data need to be entered and saved in the right format; therefore a CSV template is provided for download which helps you to enter your data in the correct format. Click CSV import/export, and then click Download CSV template. Note: CSV import requires a comma-separated CSV file.
Page 163 Now, choose Update and Save changes to save. Example case 2: The Plate definition in the Suite includes a reaction mix and some samples or controls; also, the Plate layout is partly defined. Existing parts shall not be overwritten. By using the CSV import function, residual empty wells should be filled with the same reaction mix and additional sample(s) that have not been created in section Samples &...
Page 164 Example case 4: The Plate definition in Suite includes the correct reaction mix and samples, and the Plate layout should be overwritten. The CSV file includes identical sample and reaction mix names in different order. In this case, only the option Overwrite existing data needs to be chosen for CSV import.
Page 165 Note: Both views provide information regarding the plate name, plate status, modified date, type of plate, and used disk space. 2. On a specific plate, click the three-dotted button. The context menu provides an option to edit, delete or analyze the plate. It also points you to the existing reports and enables you to export the plate.
Page 166 Note: If the plate is bigger than 2.5 GB, a tooltip next to plate size will be shown, stating that it may not be archived and exported. To get back from the search view to the general plates overview, either empty the search field and click or press Esc on your keyboard while the text field is active.
Page 167 Export and Import plate data To archive your data and free-up disk space, you can export a plate as zip file. Click the respective plate in the plates overview. On the left side of the screen, click Export plate. The plate is exported as zip file.
Page 168: Setting Up Templates
Setting up templates You can create templates to set up your experiments. You can create a new template or use an existing plate and save it as template. Note: Template creation is permission dependent. Creating a new template 1. In the Main Toolbar, click Templates, then click New template. 2.
Page 169 Saving a plate as template Follow the steps to set up a new plate as described in section Setting up an experiment. After you have entered all required information, you can save the information as a template to use for future plates.
Page 170 Using a template to create a new plate 1. In the Main toolbar, click Plates, then click on New Plate. 2. Click Plate templates, then click Import template. 3. The Import Plate Template window appears with all existing templates. 4. Select an existing template, then click Import. 5.
Page 171 Sorting You can sort the Templates Overview window by modification date or name. Sorting the templates by the modification date enables you to view the recently modified entries. Filtering You can search for specific templates by entering the name in the Search field. New template See section Setting up templates how to create a new template.
Page 172: Analysis
Analysis The QIAcuity Software Suite enables you to analyze plates that have been processed by the instrument. The following analysis options are available in the software: Absolute Quantification  Mutation Detection  Genome Editing  Copy Number Variation  Gene Expression ...
Page 173 4. Select Analyze from the context menu. Images The Software Suite also enables you to view the images. After a run is completed, you can  view the images in the Plate Analysis environment. For more information about how to access the Plate Analysis, see section Accessing the analysis environment.
Page 174 Try reimaging the plate with different exposure and/or gain settings. If the error persists, create a support package and contact QIAGEN Technical Services. Image quality control The fluorescence signal in the reference channel is measured to determine the number of valid partitions in a well.
Page 175 Note: The optimal RFU range of your positives is between 80–120 to avoid saturation and proper functioning of image analysis algorithms. Use exposure time changes (linear dependency) to optimize your RFU level. For RFU values below 60, it is recommended to increase the exposure time of the corresponding channel/s, and for RFU values below 150, it is recommended to decrease the exposure time of corresponding channel/s.
Page 176 Invalidation of images In rare cases it can happen, that the image quality is too low and the image cannot be used for further analysis. A message is shown to the customer that some wells have been invalidated. Those wells are greyed out in the plate layout and cannot be used for the result analysis. Note: This message also appears if not all wells were used in the run.
Page 177 Reasons for the invalidation of an image are: Too less fluorescence signal, for example, when the Nanoplate was re-imaged after a long  storage period (see Operating Plates for information about the storage of plates). Vibration during the imaging process can lead to blurry images. If the image of the reference ...
Page 178 Raw image of a well showing dust particles (marked with red circles) QIAcuity User Manual 06/2022...
Page 179 Signal map of the dust-corrected image In case the images still show dust particles or other particles after the correction, the recommendation is to unload the plate, wipe the plate with a lint-free tissue and to re-image the plate. Note: For QIAcuity Software Suite v2.0 or higher, dust detected in one channel is used for finding dust in other channels as well.
Page 180 Improved dust detection for dust causing light infections of neighbor partitions: left, not sufficient dust removal; right, sufficient dust removal with QIAcuity Software version 2.1. Affected area is removed from analysis and therefore partitions are invalidated. Low amplification areas In case of low amplification areas, the fluorescence signal in the target channels is less pronounced or not detectable in certain areas of the well.
Page 181 Signal map of an image with blacked-out low amplification areas Areas of bad filling Incorrect pipetting or sealing can lead to areas of the well which are not filled with reaction mix. Those areas affect the reference channel as well as the target channels and reduce the number of valid partitions.
Page 182 Signal map of an image showing areas of bad filling Crosstalk Correction algorithm Depending on the optical configuration of your QIAcuity instrument, you can detect up to two or five fluorescent channels. To compensate the spectral overlap between the fluorescent dyes, a crosstalk correction algorithm is implemented in the QIAcuity Software Suite.
Page 183 General analysis options Selecting wells for analysis To select multiple wells at the same time, click the individual wells or click one well then drag  your mouse until all wells are selected. To select all wells, click Select all. ...
Page 184 Hyperwells To achieve higher accuracy, multiple wells can be grouped together and analyzed as a single well. To define a hyperwell, select multiple wells with the same Reaction Mix and same Sample name. Then, click Group as hyperwell from the menu below or right-click and select Group as hyperwell from the context menu.
Page 185 For the analysis, hyperwells are treated as a single well but with a larger number of partitions. This may be helpful for rare event detection if the sample volume to be analyzed exceeds the volume that can be loaded into a single well. Results from all wells coupled into a hyperwell will be aggregated and presented as a result from a single well.
Page 186 2. Then, enter the number of copies/genome. You can choose the value between 1 to 99. 3. Select target of interest from the drop-down list. 4. Reference selection is now unavailable because you have not selected the wells/hyperwells with the same sample ID for the chosen reference sample. 5.
Page 187 1. First select reference sample. In this case, the user has selected sample ID 01 “patient 01”. 2. Enter the copies/genome. You can choose the value between 1 to 99. In this case, the user enters “10”. QIAcuity User Manual 06/2022...
Page 188 3. Then, select the target of interest. In the example below, the user has chosen SARS-COV-2 with channel Green. 4. The reference selection is available. From the drop-down list are two options: “01 HW2” (hyperwell with selected wells: A3, B3) or “non-hyperwell, all replicates”. In this case, the user has selected “01 HW2”.
Page 189 Note: If an imaging step failed during the run or images are of low quality, a message is shown by moving the mouse over the camera icon to indicate that the results might be incorrect. Furthermore, error messages are indicated with a red box around the image step icon, warnings with a yellow box.
Page 190 Diagram options There are several tools related to the diagrams and charts that enable you to adjust the view and download the chart you want to view. To access the tools, point to a diagram. Table 15 shows the toolbar buttons and their corresponding functions. Table 15.
Page 191 Example of a range slider below a chart. The highlighted area of the slider shows the portion of the chart that is currently displayed. The gray part of the slider is a preview of the rest of the chart. To view another part of a diagram, click the highlighted area of the slider and drag to the part that you want to view.
Page 192 2. To select the analysis type, click Target or Channel. If there are no reaction mixes available on the plate, the Target button is disabled. 3. To analyze the plate by targets, select the targets from the Select Targets list. You can select one or more targets from the list.
Page 193 List tab for absolute quantification The List tab contains a table with an overview of the analyzed wells. The following columns are available in the table: Well ID – like A1, B2  Reaction mix – This column contains the icon and the name of the reaction mix. ...
Page 194 Current results If you want to export current results of analysis please follow the instruction below. 1. Select wells that you want to get results for. 2. Choose if you want to analyze per Target or per Channel by clicking proper tab, and select targets or channels you are interested in.
Page 195 Categories – names of channels  Group – identifier of channels from the Categories column, which are positive (+) and  negative (−) Count – number of valid partitions in a certain group  Total – total number of valid partitions in a well ...
Page 196 Status Code Poisson test – linear distribution issue Blistering (Poisson test) Poisson test areas of too low/no positive partitions amplification) Poisson test areas of too high positive partitions (Proportion test – over-amplification) 3 & 4 Reference partition not filled 5 & 9 Dust detected by different reasons 6, 7, 8 Artifacts founds...
Page 197 Analyze section with 2 Replicate groups (A11, B11) and (A12, B12). Replicates are analyzed as separate wells but in addition the mean concentration value and the CI value of the mean concentration are provided on demand. On the right side above the table there is a checkbox, which allows the user to show mean values for replicates in addition.
Page 198 List tab with selected mean values for replicates. Signalmap tab for absolute quantification The Signalmap tab provides positive partitions for target channels and valid partitions for the reference channel of selected wells. For each channel included in target or channel selection, one signal map view is created.
Page 199 Signal map for a target channel. On mouse-click, a modal window opens containing the well image providing following tool options on top of the right side. Zoom In  Zoom out  Download signal map of this well as picture ...
Page 200 Zoom window for a well image of the reference channel. Heatmap tab for absolute quantification The Heatmap tab shows the concentration of the selected targets or channels in each well. The values of all selected wells are also displayed in this tab. Values for disabled wells are not displayed.
Page 201 Showing the mean concentration values for replicates in the concentration view click Show mean values for replicates. Mean values are not supported in the partition view. Therefore, the related check box is greyed out in the partition view and a meaningful information message is shown to the user.
Page 202 The partitions heatmap of a selected target. To add any of the heatmaps to the report, click Add to report next to the relevant diagram. For more information on reports, see section Reports. Heatmap for hyperwells QIAcuity User Manual 06/2022...
Page 203 Histogram tab for absolute quantification The Histogram tab displays graphs that visualize the fluorescence values of selected wells for the selected target or channel. One histogram view is created for each selected target or channel. The histograms are sorted by channel position in imaging (“Green”, “Yellow”, “Orange”, “Red”, “Crimson”), separated by a horizontal line.
Page 204 Note: For a multi-well selection the user can’t change the thresholds individually. The new threshold becomes valid for all selected source wells. In case the user would like to change thresholds individually he has to change the source selection to a single well selection To reset the software to automatically recalculate the threshold, click Automatic threshold Again, click Recalculate to apply the auto-threshold to the results.
Page 205 Reference channel The Software Suite also provides a histogram for the reference channel, which is hidden by default. To view the histogram for the reference channel in addition, click Show reference channel. The title of this histogram indicates that the histogram is related to the reference channel. The graph for the reference channel provides the possibility to set a common lower as well as upper threshold to exclude partitions with too high/too low RFU.
Page 206 1D Scatterplot tab for absolute quantification The 1D Scatterplot tab shows one 1D Scatterplot view for each analyzed target or channel. If there are more than one scatterplot views, they are separated with a horizontal line. The 1D Scatterplot views are sorted by channel position in imaging (“Green”, “Yellow”, “Orange”, “Red”, “Crimson”). The title of a 1D Scatterplot view shows the related channel name including the dot channel color indicator, and if defined also the target name.
Page 207 To open an individual plot, click on the appropriate header of the 1D Scatterplot. QIAcuity User Manual 06/2022...
Page 208 Changing the threshold individually per well 1. To change the threshold individually per well, click on the appropriate header of the well in the 1D Scatterplot view. A window opens and the threshold can be changed by pointing over the chart, which triggers the appearance of a dotted line. 2.
Page 209 Changing the thresholds for all selected wells (Common threshold) The 1D Scatterplot view provides an option to set a common threshold value for all associated wells using the Common threshold input field. Click the Recalculate button to trigger the re-analysis of data Use the Auto-threshold button to re-set the threshold to the values, which is calculated by the analysis algorithm for each well.
Page 210 Reference channel The Software Suite also provides a 1D Scatterplot view for the reference channel, which is hidden by default. To view the 1D Scatterplot for the reference channel in addition, click Show reference channel. The title of this 1D Scatterplot view indicates that the 1D Scatterplot is related to the reference channel.
Page 211 2D Scatterplot tab for absolute quantification The 2D Scatterplot tab shows a 2D Scatterplot view that displays the fluorescence intensity from two selected channels or targets, allowing for a comparison and evaluation of the fluorescence from two sources. To create a 2D Scatterplot, assign targets or channels to the x-axis and y-axis using the drop-down menus.
Page 212 For each partition point the assignment to its group (++, +−, −+, or −−) is derived from the threshold values. The partition number of each group is displayed on the top right, next to the diagram. The color of the point that represents the partition fluorescence measurement is assigned according to the group.
Page 213 Assigning partitions to a group using free hand (Lasso mode) The user can select Lasso partitions measurement dots with a free hand mode, so that he can use a more precise assign partitions group they should belong The lasso is available in menu above the diagram on hover. When the user clicks lasso icon ( he can first select the group (++, +−, −+, or −−) to which partitions should be assigned using the free hand mode.
Page 214 Concentration diagram tab for absolute quantification The Concentration Diagram tab shows the diagrams that display the distribution of concentration values and confidence intervals. One diagram is created for each selected target or channel. A concentration diagram has two axes. The x-axis shows the analyzed wells, and the y-axis represents the concentration values for the selected targets or channels of each well.
Page 215 Mutation detection The Plate Analysis environment of the Software Suite includes the Mutation Detection tab. Mutation Detection analysis is based on the concentrations (see Absolute quantification). To use Mutation detection, the definition of targets in the reaction mixes and samples is mandatory. For more information, see section Setting up an experiment.
Page 216 List tab for mutation detection The List tab contains a table with an overview of the analyzed wells. The following columns are available in the table. Well ID – This column represents the well position in the plate layout.  Sample/NTC/Control –...
Page 217 Simplex test (configured wildtype and mutant targets are part of different reaction mixes):  For tests having the configured targets in different wells/reaction mixes, the mean result is calculated and shown for replicates in the selection. The list view is extended by 4 columns indicating following mean values: Mean concentration value ...
Page 218 To add the heatmap diagram to the report, click Add to report. For more information on reports, see section Reports. Point diagram tab for mutation detection The Point diagram tab shows a diagram that displays the percentage of mutant fractions in each analyzed well.
Page 219 the x-axis. This is also applied in same way by default to the view where targets are in different reaction mixes. In this case individual replicate results are not available. Mean values are calculated and shown over all replicates that are included in the well selection only. If there are further replicates of the same sample which are not included in well selection, they are not considered in the calculated mean value and show their individual results values.
Page 220 confidence interval. To view detailed information, hold the mouse pointer over its corresponding bar. A tooltip with detailed information opens. To view the mean values for replicates on the concentration diagram, click Show mean values for replicates. If the user clicks into the checkbox to select the mean representation for replicates, the bars of individual replicates disappear and only one bar is shown at the sample label that represents the mean mutant fraction value of the replicates.
Page 221 1. Click the applicable wells in the plate layout. For more information, see Selecting wells for analysis. 2. Select the applicable wild-type target from the Wild type target list. 3. Select the applicable edited target from the Edited target list. 4.
Page 222 Replicates are treated different for multiplex and simplex test setup: Multiplex test (configured wildtype and edited targets are part of the same reaction mix):  Replicates are analyzed as separate wells but in addition the mean concentration and mean edited fraction values together with the corresponding mean CI are provided on demand. On the right side above the table there is checkbox, which allows the user to show mean values for replicates in addition.
Page 223 Heatmap tab for genome editing The Heatmap tab contains heatmaps that show the edited fraction as percentage in each of the wells. If a well is not selected as a source for the analysis, the value is not displayed on the heatmap and its background color is gray.
Page 224 To view an additional toolbar that enables actions related to the diagram, such as downloading the plot, hold the pointer over the diagram. For more details about the toolbar, refer to section Diagram options. The y-axis scale can be modified using the buttons located below each graph. The buttons are visible when you hold the pointer over a graph.
Page 225 Concentration diagram tab for genome editing The Concentration diagram tab shows a diagram that displays the distribution of concentration values in the wells together with their confidence intervals A concentration diagram has two axes. The x-axis shows the analyzed wells and samples, and the y-axis represents the concentration values.
Page 226 To add the Concentration diagram to the report, click Add to report. For more information on reports, see section Reports. Copy number variation The Copy number variation tab contains diagrams that visualize the data related to copied genes in targets of interest comparing to reference targets or reference sample. The analysis results are put into list views, heatmaps, point diagrams, and concentration diagrams.
Page 227 2. Select the applicable reference target from the Reference target list. 3. Enter the number of copies per genome in the Copies/genome field for the target of interest in reference sample. The value should be between 1 and 99. 4. Select the applicable target that you want to investigate from the Target of interest list. QIAcuity User Manual 06/2022...
Page 228 5. Select one or more reference targets from the Reference target(s) list. Note: You can select more than one reference target. 6. To view the results of the analysis, click Show results. 7. The results are divided into several tabs. To view the contents of the tab, click the tab title. Setting up a copy number variation analysis with reference sample 1.
Page 229 3. Enter the number of copies per genome in the Copies/genome field for the target of interest in reference sample. The value should be between 1 and 99. 4. Select the applicable target that you want to investigate from the Target of interest list. 5.
Page 230 List tab for copy number variation The List tab contains a table with an overview of the analyzed wells. The following columns are available in the table: Well ID – This column represents the well position in the plate layout. ...
Page 231 Replicates are treated different for multiplex and simplex test setup: Multiplex test (configured target of interest and reference targets are part of the same reaction  mix): On the right side above the table there is checkbox which allows the user to show mean values for replicates in addition.
Page 232 Heatmap tab for copy number variation The Heatmap tab contains a heatmap that shows the number of copies per genome in each of the wells. If a well is not selected as a source for the analysis, the value is not displayed on the heatmap and its background color is gray.
Page 233 Point diagram tab for copy number variation The Point diagram tab shows the diagram that displays the number of copies per genome of the configured copy number variation test and the confidence intervals related to every value. A point diagram has two axes. The x-axis shows the analyzed wells and samples, and the y-axis represents the number of copies in each genome.
Page 234 Note: If the selected analysis source contains replicates of the reference sample, your results can be calculated only by using mean results for replicates. In this case the “Show mean values for replicates” check box is marked and can’t be changed. In this case there is a warning message above the diagram title and when the user hover on the checkbox, a tooltip appears to inform the user that selected analysis source contains replicates of the reference sample and result is calculated only by using mean results for replicates.
Page 235 The Concentration diagram is a bar plot which presents two values: Concentration value as bar  CI value as error bar  The diagram consists of all possible combinations. Each sample on the diagram is represented by the number of bars according to targets that was selected as a target of interest and reference targets.
Page 236 To add the Concentration diagram to the report, click Add to report. For more information on reports, see section Reports. Gene expression The Software Suite analyzes the gene expression of the samples. The analysis results are put into list views, heatmaps, point diagrams, and concentration diagrams Note: Saving gene expression tests is not yet provided.
Page 237 Well ID – This column represents the well position in the plate layout.  Sample/NTC/Control Name – This column shows the sample, NTC, or control name with its  corresponding icon that identifies the sample or indicates whether the entry is an NTC or control.
Page 238 For tests having the configured targets in different wells/reaction mixes, the mean result is calculated and shown for replicates in the selection. The list view is extended by 4 columns indicating following mean values: Mean concentration value  CI (95%) − CI of mean concentration as percentage ...
Page 239 tooltip appears to inform the user that selected analysis source contains replicates of the reference sample and result is calculated only by using mean results for replicates. To add the heatmap to the report, click Add to report. For more information on reports, see section Reports.
Page 240 QIAcuity User Manual 06/2022...
Page 241 In case that targets are in different reaction mixes each combination of target of interest and reference targets in one sample throughout the selected wells is shown in the diagram with a point for the result. To view detailed information, hold the mouse pointer over its corresponding point. A tooltip with detailed information opens.
Page 242 Concentration diagram for gene expression The concentration diagram tab shows the diagram that displays the concentration values of the configured gene expression test and the confidence intervals related to every value. A concentration diagram has two axes. x-axis represents labels of wells and samples that it belongs to ...
Page 243 To view the mean values for replicates on the diagram, click Show mean values for replicates. When this checkbox is checked, the concentration diagram shows bars that represents mean concentration value for replicates within the well selection. The label below the bar shows well positions that are included in the replicates value of mean concentration.
Page 244: Reports
Reports In the QIAcuity Software Suite, you can create reports about your analysis results of a plate. All created reports remain accessible in the Software Suite and can be downloaded. Only unsigned reports can be deleted. Creating a new report for a plate from the analysis environment To create a new report from your analysis results, follow these steps: 1.
Page 245 Pre-view window of selected diagrams to be added in the report. 5. Use the icon to delete a diagram from the list of your diagrams to be included in the report. Use the icon in the preview window or icon in the footer to close the preview window.
Page 246 Create Report view for a plate 8. Use this table as a guide in selecting which information to include in the report. Table 17. Report elements Element Description Report name Every Report needs a specific name, without special characters ~ ‘ “ ! @ ^( ) = [ ] { } : ; , < > | \ . Run details User, Start and end time, Run steps, Run status, Software and Instrument version, dPCR steps...
Page 247 9. To finish creating the report, click Generate report PDF. 10. The report is created and automatically downloaded in the download folder of the PC as a .pdf file. The report is stored in the SW Suite and accessible via the report environment of the plate.
Page 248: Sign Report
Use this table as a guide in managing your created reports for a specific plate. Table 18. Action button in report environment Action Description Downloads the report in PDF format Delete the report Back to Analysis view of this plate Back to plates overview screen Add signature to the report List signers: user name, role, date, and reason...
Page 249 3. A pop-up window will appear with confirmation request. You need to download and read report, then add your password and a reason for signing and finally click Sign the report button. 4. When the report is signed, an appropriate message will be presented on the report list and the Add signature button will be disabled.
Page 250 When the report is signed, a user has an option to check who has performed such action. To do so, please follow the instructions below: 6. Go to report list view. 7. Click on the Check signers button. 8. A pop-up window will appear with information about signers. Name and surname Login in brackets Role...
Page 251: Archive
Archive In the QIAcuity Software Suite, you can set up an archive on an external drive. This will allow you to store your old plates and save disk space on the laptop. Only the user with Administrator role can set up an archive; to do so, go to Configuration and select the Archive tab: When Add archive is clicked, the user can provide a path to the external drive and click Save.
Page 252 Note: Automatic archiving is running at 1 am every day. Manual plate archiving When the archive is set up, the user can manually move the plate to the archive. To do so, click the three dots (...) on any of the plates on the Plates Overview screen and select Archive. The plate will be moved to the archive.
Page 253 Browsing archive You can browse and search plates in the archive. Go to Archive on the Navigation bar. You can then see all your archived plates. Recovering a plate from the archive To recover plates from the archive, go to the Archive screen and click on the three dots (…) on any of the plates on the Archive screen and select Restore.
Page 254: Re-Analysis Of Plates After Qiacuity Software Suite Version Upgrade
Re-analysis of plates after QIAcuity Software Suite version upgrade After upgrading the QIAcuity Software Suite (see Installation Procedure and Upgrading QIAcuity Software Suite to new version), the status of all plates will be changed to Read-only unless the plates are upgraded to the new software version. The same applies to plates that are imported from a previous Software Suite version.
Page 255: Volume Precision Factor (Vpf)
Note: Multiple plate batches can come from one microstructure molding form. New sets of VPFs will be published during production of the Nanoplate batches and can be downloaded from the QIAGEN website. Identification of plates without VPF Plates without VPF can be analyzed using an average volume per plate. Such plates are marked with the VPF (target) icon on the Plates Overview screen.
Page 256 In addition, information about missing VPF is available on the Plates Overview screen, Analysis screen, Plate Configuration screen, and on the Report. Loading VPF All existing VPFs to all used microstructure negative forms are bundled together in one zip file to allow the user to always download the most recent version.
Page 257 Once clicked, a new pop-up window will show up with detailed instruction: Please follow the steps to properly load the most recent VPF file. Note: The structure of the VPF file is a zip file. There is no need to extract it. When loaded, the QIAcuity Software will refresh all plates and align concentrations using the newly loaded well-based volumes.
Page 258: Operating Plates
In the QIAcuity plate-based system, one reaction mix per well is partitioned into a large number of individual partitions prior to the amplification step, resulting in one or very few templates being present in each partition. QIAGEN offers three different plate types according to specific user needs.
Page 259: Qiacuity Nanoplate 26K 24-Well
QIAcuity Nanoplate 26K 24-well For applications requiring high sensitivity, QIAGEN offers the 26K nanoplate. In this plate, one reaction mix is distributed over 4 wells and separated into approximately 26000 partitions. The plate may be used for up to 24 samples and has a blue frame as distinction from the other plates.
Page 260: Qiacuity Nanoplate 8.5K 96-Well
For better handling of the plate, you can place the plate into the Nanoplate tray that can be ordered as accessory, see Appendix B – QIAcuity Accessories or the QIAcuity webpage on www.qiagen.com. To set up a plate, follow these steps: 1.
Page 261 Refer to the Application guide on www.qiagen.com for the recommended restriction enzymes. Important: Do not pipet master mix and sample separately into the Nanoplate as this will lead to insufficient mixing. 2. Pipet each reaction mix from the pre-plate into a well of the Nanoplate. If possible, use an electric one-channel pipette.
Page 262 Note: For a properly sealed plate, the plate seal covers the whole structure, and no bubbles or strong depressions are visible, as this can also lead to bad filling of the wells. 8. The plate frame gives the option to mark the plate with a marker pen. Use the lane between the plate edge and the printed letters (next to column 1) as well as the mirrored part (from column 12 to the plate edge) only.
Page 263: Maintenance Procedures
Maintenance Procedures WARNING/ Risk of personal injury and material damage [W1] CAUTION Only perform maintenance that is specifically described in this user manual. The following maintenance procedures must be carried out to ensure reliable operation of the QIAcuity: Regular maintenance ...
Page 264 General cleaning of the QIAcuity: Mild Detergents (e.g., Mikrozid ® AF sensitive)  25% ethanol  Disinfection Ethanol-based disinfectants can be used for disinfection of surfaces: e.g., 25 g ethanol and 35 g 1-propanol per 100 g liquid or Mikrozid Liquid (Schülke & Mayr GmbH, cat. no. 109160). Disinfectants based on glyoxal and quaternary ammonium salt can be used, e.g., 10 g glyoxal, 12 g lauryldimethylbenzylammonium chloride, 12 g myristyldimethylbenzylammonium chloride, and 5–...
Page 265 Ensure that no liquid runs down the touchscreen. Liquid may be drawn through the dust  protection sealing by capillary forces and cause malfunction of the display. To clean the touchscreen, moisten a soft lint-free cloth with water, ethanol, or a mild detergent and carefully wipe the display.
Page 266: Servicing
Do not use bleach to clean or disinfect the QIAcuity. WARNING Toxic fumes [W23] Do not use bleach to disinfect used labware. Servicing Contact QIAGEN Technical Services or your local distributor for more information about flexible Service Support Agreements from QIAGEN. QIAcuity User Manual 06/2022...
Page 267: Regular Maintenance Procedure Of Qiacuity
Improper use of the QIAcuity may cause personal injuries or damage to the instrument. The QIAcuity must only be operated by qualified personnel who have been appropriately trained. Servicing of the QIAcuity must only be performed by a QIAGEN Field Service specialist. Regular maintenance procedure of QIAcuity Clean the instrument on a regular basis, especially if fluids have been spilled on the instrument.
Page 268 3. Remove the filter from the swing-out filter compartment. 4. Replace with a new filter and push the compartment to the top to close. QIAcuity User Manual 06/2022...
Page 269: Decontaminating The Qiacuity
If damaged plates were used and the inside of the instrument is contaminated, please contact QIAGEN Technical Services. The QIAcuity should also be decontaminated before shipping (e.g., back to QIAGEN). In this case, a decontamination certificate must be completed to confirm that the decontamination procedure has been carried out.
Page 270 3. To clear the data, tap Force clear images. Click OK in the confirmation dialog box to delete the data. Images from the system and database will be removed. QIAcuity User Manual 06/2022...
Page 271: Regular Maintenance Procedure For Qiacuity Software Suite
Regular maintenance procedure for QIAcuity Software Suite To monitor the space of your disk, click Disk monitor in the main toolbar. This shows an overview about the state of the disk, disk name, and disk path. It also shows the remaining free space and total space of your disk.
Page 272: Troubleshooting
Serial number of the instrument  This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue. Note: For most cases, to allow proper analysis of an error situation, the support package either from the instrument and/or the Suite is required.
Page 273: Creating A Support Package With The Qiacuity Instrument Software
3. Tap Quick Test or Extended Test depending on the type of test you want to perform. 4. The instrument starts the test. The ongoing actions and their statuses are shown in the Log File Preview pane. The log from the test can be downloaded as part of a support package. For more information about support package, see Creating a support package.
Page 274 3. In the Support package pane, select Upload to Software Suite. 4. To set the timeframe of the support package, tap Set timeframe . The default timeframe is the last two weeks. 5. Tap the applicable option for your preferred timeframe. Tap Today to create a support package for the current day.
Page 275 To create a support package and save it on a USB drive, follow the steps below. 1. On the toolbar, tap Tools 2. Tap Data Management. 3. Connect a USB drive to the instrument. Wait for the device to detect the USB drive. A notification is displayed once the drive is connected.
Page 276 Tap Today to create a support package for the current day.  Tap Last week or Last 2 weeks to select either the last week or the last two weeks.  To set a custom timeframe, tap From and select a start date from the calendar. Then, tap ...
Page 277: Creating A Support Package With The Qiacuity Software Suite
Creating a support package with the QIAcuity Software Suite 1. Select a plate in the plates overview. 2. Click the left side of the screen on Support Package. A new window pops up, where you can specify what kind of support package you want to generate and from which time frame.
Page 278 Note: The standard package includes the log files and a combined image view of all wells. The extended package includes the log files and the raw images of each single well. By clicking on the calendar symbol, you can define the time frame from which the support package shall be generated.
Page 279: Troubleshooting The Instrument And Software
A missing Plate Seal is detected by the instrument. Please ensure that always a closed plate with Plate Seal is loaded into the instrument. A run cannot be started if a plate seal is not detected by the instrument. Only use QIAGEN products for closing the plates. Drawer blocking...
Page 280 Ensure that the USB stick is not inserted in the USB port. Power ON the QIAcuity again. The login screen should be visible. If the error persists, contact QIAGEN Technical Services. Error displayed when inserting the...
Page 281 Fetch error while accessing the Users If such error occurs, please contact QIAGEN Technical Service in list in User Management order to solve the issue. Software The QIAcuity Software Suite does not Check that the software is installed on the laptop.
Page 282: Accessing The System Status And Clearing Errors
Please turn on the instrument for 30−60 min. After this time clear the Error and restart. The instrument should start without an Error. If the Error persists, please contact QIAGEN Technical Services. Error 33 The error can occur if the instrument was shut down with plates loaded to all plate slots or an error occurred in a fully loaded instrument.
Page 283 6. Restart the instrument. The instrument initializes and all modules are returned to their home positions. Note: If the affected module is not working after you cleared the error and restarted the instrument, contact QIAGEN Technical Services. QIAcuity User Manual 06/2022...
Page 284: Glossary
Separation of the single partitions filled with the reaction volume Support Package Information wrapped up in a *.zip file to be sent via an email program to QIAGEN Technical Services to inform QIAGEN what went wrong at the customer's site and how to help the customer...
Page 285: Appendix A - Technical Data
Appendix A – Technical Data QIAGEN reserves the right to change specifications at any time. Operating conditions Power 100–240 V AC, 50/60 Hz, Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages. Maximum power consumption: QIAcuity One, 2plex: 1000VA...
Page 286: Transport Conditions
Transport conditions Air temperature –25ºC to 60ºC (–13ºF to 140ºF) in manufacturer’s package Relative 5% to 85% (non-condensing) humidity Environmental 2K11 & 2M4 (IEC 60721-3-2) class Ambient 700hPa to 1060hPa pressure Storage conditions Air temperature 5ºC to 40ºC (41ºF to 104ºF) in manufacturer’s package Relative humidity 5% to 85% (non-condensing) Environmental...
Page 287 Process temperature: 35°C to 99°C Thermal Accuracy: ±1°C Ramp rate: approx. 3.0°C/s specifications Homogeneity (over plate surface): ±1°C The QIAcuity Eight features two Thermocyclers that are operated in parallel The 2-plex version features the channels Green and Yellow and the 5-plex Optical version all following channels: specifications...
Page 288 Handheld Scan Pattern: Area Image (1280 x 800-pixel array) scanner Motion Tolerance: Up to 89 cm/s (35 in/s) (optional) Print Contrast Ratio: 15% (minimum) Decode Capability: Reads standard 1D, 2D, Postal and stacked codes Resolution: 1D Linear: 0.102 mm/4 mils; PDF417: 0.127 mm/5 mils; Data Matrix: 0.195 mm/7.5 mils QIAcuity User Manual 06/2022...
Page 289: Declaration Of Conformity
Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE-marked electronic equipment is provided.
Page 290: California Proposition 65
Representations and warranties made by any person, including representatives of QIAGEN, which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN.
Page 291 Table 21. Marking for the restricted use of hazardous substances in electronic and electrical products (SJ/T 11364-2014) Part Name - 部件名称 Toxic or Hazardous Substances and Element - 有毒或有害的物 Lead (Pb) 铅 Mercury (Hg) Cadmium Hexavalent Polybrominat Polybrominat (Cd) 镉 Chromium ed biphenyls ed diphenyl...
Page 292: Appendix B - Qiacuity Accessories
* Additional instrument and Service bundles are available. † For all systems, Installation and Training is included but are additionally available as separate service offerings. For specific catalog numbers and additional information, visit www.qiagen.com or contact your local sales representative. QIAcuity User Manual 06/2022...
Page 293 IQ/OQ Service is an on-site qualification service provided by a certified QIAGEN Service Specialist. This includes labor and travel Barcode Hand Scanner, QIAcuity Separate 2D barcode scanner for...
Page 294 EvaGreen Mastermix, 4 x 20 ml Water For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available atwww.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.
Page 295: Appendix C - Informations De Sécurité
L’entretien du QIAcuity ne doit être effectué que par un spécialiste de l’entretien sur site QIAGEN. Procéder à la maintenance comme décrit dans la section Procédures de maintenance. QIAGEN facture les réparations dues à une maintenance incorrecte. QIAcuity User Manual 06/2022...
Page 296 [W4] Le QIAcuity est conçu pour être utilisé avec les réactifs et les substances fournis avec les kits QIAGEN ou autrement que de la façon décrite dans le mode d’emploi correspondant. L’utilisation d’autres réactifs et d’autres substances peut provoquer un incendie ou une explosion.
Page 297: Sécurité Électrique
Si un liquide s’est répandu à l’intérieur de l’appareil, l’éteindre, le déconnecter de la prise  secteusr et prendre contact avec les services techniques de QIAGEN. Si l’appareil présente un danger électrique, empêcher le reste du personnel de s’en servir et contacter les services techniques de QIAGEN.
Page 298: Environnement
L’appareil peut présenter un danger électrique dans les cas suivants : L’appareil ou le câble d’alimentation semble être détérioré.  Il a été stocké dans des conditions défavorables pendant une longue période.  Il a subi des chocs sévères durant le transport. ...
Page 299 Échantillons Les échantillons peuvent contenir des agents infectieux. Vous devez connaître le risque pour la santé que ces agents représentent et vous devez utiliser, stocker et mettre au rebut ce genre d’échantillons conformément aux règles de sécurité nécessaires. AVERTISSEMENT Échantillons contenant des agents infectieux [W9] Certains échantillons utilisés avec cet appareil peuvent contenir des agents infectieux.
Page 300: Produits Chimiques
Produits chimiques AVERTISSEMENT Produits chimiques dangereux [W10] Certains produits chimiques utilisés avec cet appareil peuvent être dangereux ou le devenir après l’exécution du cycle du protocole. Toujours porter des lunettes de protection, des gants et une blouse de laboratoire. La personne responsable (par exemple, le directeur de laboratoire) doit prendre les précautions nécessaires afin de garantir que le lieu de travail environnant est sûr et que les opérateurs de l’appareil ne sont pas exposés à...
Page 301: Sécurité Contre Les Rayonnements
Sécurité contre les rayonnements Risque de dommages corporels [W13] AVERTISSEMENT Lumière laser avec niveau de danger 2 : Ne pas regarder fixement le faisceau lumineux lors de l’utilisation du lecteur de code-barres portable. Symboles sur le QIAcuity Symbole Emplacement Description Plaque signalétique à...
Page 302: Appendix D - Sicherheitshinweise
Die Instandhaltung des QIAcuity darf nur durch einen Service-Spezialisten des QIAGEN Außendienstes durchgeführt werden. Führen Sie alle Wartungsarbeiten gemäß den Anweisungen im Abschnitt Wartungsarbeiten dieses Handbuchs durch. QIAGEN stellt Reparaturen, die auf nicht fachgerecht durchgeführte Wartungsmaßnahmen zurückzuführen sind, in Rechnung. QIAcuity User Manual 06/2022...
Page 303 WARNUNG Explosionsgefahr [W4] Der QIAcuity darf ausschließlich mit Reagenzien und Substanzen aus den QIAGEN Kits bzw. gemäß den Angaben in der entsprechenden Gebrauchsanweisung verwendet werden. Die Verwendung anderer Reagenzien und Substanzen kann zu einem Brand oder zu einer Explosion führen.
Page 304: Schutz Vor Stromschlag
VORSICHT Beschädigung des Geräts [C5] Lehnen Sie sich nicht an den Touchscreen, wenn er herausgezogen ist. Schutz vor Stromschlag Hinweis: Ziehen Sie das Netzanschlusskabel aus der Steckdose, bevor Sie Instandhaltungs- /Wartungsarbeiten an einem Gerät vornehmen. WARNUNG Stromschlaggefahr [W5] Jede Unterbrechung des Schutzleiters (Erdungs- bzw. Masseleiter) im Gerät oder außerhalb des Geräts und jede Abtrennung des Schutzleiters am Anschluss der Netzleitung erhöht die Gefahr eines Stromschlags.
Page 305: Umgebung
Falls Flüssigkeit auf dem Gerät verschüttet wird und hineinläuft, schalten Sie es sofort AUS,  ziehen Sie den Netzstecker und setzen Sie sich mit dem Technischen Service von QIAGEN in Verbindung. Falls die elektrische Sicherheit bei der Bedienung des Geräts nicht mehr gewährleistet werden kann, muss das Gerät gegen Benutzung durch darüber nicht informiertes Personal gesichert werden.
Page 306: Biologische Sicherheit
Biologische Sicherheit Bei Substanzen und Reagenzien, die humanes Untersuchungsmaterial enthalten, sollte immer von einer möglichen Infektionsgefahr ausgegangen werden. Wenden Sie nur sichere Laborverfahren an, wie sie z. B. in Veröffentlichungen wie Biosafety in Microbiological and Biomedical Laboratories HHS, (www.cdc.gov/labs/BMBL.html) beschrieben sind. Proben Proben können infektiöse Erreger enthalten.
Page 307: Chemikalien
Chemikalien WARNUNG Gefährliche Chemikalien [W10] Einige Chemikalien, die mit diesem Gerät verwendet werden, können gefährlich sein oder nach Beendigung eines Protokolllaufs gefährlich werden. Tragen Sie immer eine Schutzbrille, Laborhandschuhe und einen Laborkittel. Die verantwortliche Person (z. B. der Laborleiter) muss alle erforderlichen Vorsichtsmaßnahmen treffen, um sicherzustellen, dass die unmittelbare Umgebung des Arbeitsplatzes sicher ist.
Page 308: Strahlensicherheit
Strahlensicherheit Gefahr von Personenschäden [W13] WARNUNG Laserlicht der Gefahrenklasse 2: Schauen Sie bei Verwendung des Barcode- Handscanners nicht in den Laserstrahl. Symbole auf dem QIAcuity Symbol Beschreibung Typenschild an der CE-Markierung der EU-Konformität Geräterückseite Typenschild an der Symbol der CSA-Zertifizierung in Kanada Geräterückseite und den USA Typenschild an der...
Page 309: Appendix E - User Management Permissions
Appendix E – User management permissions The table below shows all available permissions for the user management including short descriptions and technical names. These technical names are shown in audit trail details in case a user has made changes to any users or roles. Permission Technical name Description...
Page 310 Read Plate QIACUITY_PLATE_ALL_READ User can search for specific Plate, see all created Plates, analyze a Plate, check details about a Plate (dPCR parameters, plate layout (samples, reaction mixes, controls)) and export Plate to CSV. Delete Plate SUITE_PLATE_ALL_DELETE User can delete any Plates. Owned plates Run Experiment CSW_PLATE_OWNED_RUN...
Page 311 Templates [Instrument and PC software] Create Template SUITE_TEMPLATE_ALL_CREATE User can create new Template. Edit Template SUITE_TEMPLATE_ALL_EDITDATA User can edit existing Template. Read Template SUITE_TEMPLATE_ALL_READ User can read information about existing Templates and use them while creating and editing plates (if also has appropriate plate permissions).
Page 312: Document Revision History
Document Revision History Date Changes July 2020 Initial release March 2021 Implemented changes for software updates (QIAcuity Instrument SW 1.0 and QIAcuity Software Suite 1.2). Replaced multiple images to cater changes with the software updates. Updated “Powering ON the QIAcuity” section. Added new section: “Upgrading QIAcuity Software Suite to new version”.
Page 313 QIAGEN specifically disclaims any other licenses, expressed or implied other than those expressly stated. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.For updated license terms, see www.qiagen.com.
Page 314 Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com QIAcuity User Manual 06/2022...

This manual is also suitable for:

Qiacuity 911020 Qiacuity 911040 Qiacuity 911050 Qiacuity911000911020 ... Show all

Qiagen QIAcuity 911000 User Manual

Quick Links

Troubleshooting

Need help?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Qiagen QIAcuity 911000

Summary of Contents for Qiagen QIAcuity 911000

This manual is also suitable for:

Table of Contents