User Manuals: Qiagen 9002042 Real-time PCR Kit

Manuals and User Guides for Qiagen 9002042 Real-time PCR Kit. We have 1 Qiagen 9002042 Real-time PCR Kit manual available for free PDF download: User Manual

Qiagen 9002042 User Manual (208 pages)

Brand: Qiagen | Category: Laboratory Equipment | Size: 16 MB

Table of Contents
2
Introduction
8
About this User Manual
8
General Information
9
Technical Assistance
9
Policy Statement
9
Version Management
10
Intended Use of the Rotor-Gene Q MDX
10
Requirements for Rotor-Gene Q MDX
10
Materials Required
11
Materials Required but Not Provided
11
Safety Information
12
Proper Use
13
Electrical Safety
15
Biological Safety
16
Chemical Safety
17
Waste Disposal
18
Mechanical Hazards
18
Maintenance Safety
20
Symbols on the Rotor-Gene Q MDX
21
General Description
22
Rotor-Gene Q MDX Principle
22
Thermal Performance
22
Optical System
23
Available Channels
24
External Features of the Rotor-Gene Q MDX
25
Air Vents on the Lid
25
Lid Handle
25
Rotor Chamber
25
Instrument Status Lights
25
Internal Features of the Rotor-Gene Q MDX
26
Rotor Hub
26
Optical Lens
26
Optical Lens Were the Excitation Diode Light Is Focused on the Tubes
26
Installation Procedures
27
System Delivery and Installation
27
Unpacking the Rotor-Gene Q MDX
27
Hardware Installation
28
Software Installation
29
Software Version
32
Additional Software on Computers Connected to Rotor-Gene Q MDX Instruments
32
Site Requirements
39
AC Power Connection
40
Power Requirements
40
Grounding Requirements
40
Installation of AC Power Cord
40
Configuration for Windows Security
40
Workstation Requirements
42
Unpacking and Installing the Rotor-Gene Q MDX
42
Software Upgrade
43
Accessories
44
Repackaging and Shipping the Rotor-Gene Q MDX
44
Getting Started
44
Powering on the Rotor-Gene Q MDX and Workstation
44
Operating Procedures
45
Use of the Rotor-Gene Q MDX Software
45
Quick Start Wizard
45
Advanced Wizard
49
Use of the Rotor-Gene Q MDX Hardware
67
Rotor Types
67
Reaction Setup
70
Rotor-Disc Setup
73
Analysis User Interface
77
Workspace
77
Toolbar
77
View Raw Channels
77
Toggling Samples
78
File Menu
80
New
80
Open and Save
81
Reports
82
Setup
83
Analysis Menu
83
Analysis
83
Quantitation
85
Two Standard Curve
96
Delta Delta C Relative Quantitation
100
Melt Curve Analysis
103
Comparative Quantitation
106
Allelic Discrimination
108
Scatter Graph Analysis
109
Endpoint Analysis
111
Concentration Analysis
117
High Resolution Melt Analysis
120
Run Menu
121
Start Run
121
Pause Run
121
Stop Run
122
View Menu
122
Run Settings
122
Temperature Graph
125
Profile Progress
126
Edit Samples
127
Display Options
132
Access Protection for Rotor-Gene Q Software
133
Configuration for Windows 7
134
Configuration for Windows 10
139
Running Multiple Users on the same Computer
141
Audit Trails
142
Run Signatures
144
Sample Locking
145
Locked Templates
147
Gain Menu
147
Window Menu
148
Help Function
148
Send Support E-Mail
149
Additional Functions
153
Analysis Templates
153
Opening a Second Run
153
Scaling Options
153
Exporting Graphs
154
Spanner/Wrench Icon
157
Selected Area Options
158
Maintenance
159
Cleaning the Rotor-Gene Q MDX Surface
159
Decontaminating the Rotor-Gene Q MDX Surface
160
Rotor-Gene Q Repair
160
Optical Temperature Verification
161
OTV Principle
161
Rotor-Disc OTV Kit Components
161
Running an OTV
162
High Resolution Melt Analysis
165
Instrumentation
166
Chemistry
167
SNP Genotyping Example
167
Methylation Analysis Example
169
Guidelines for Successful HRM Analysis
170
Sample Preparation
172
Software Setup
172
Real-Time PCR Data Analysis
178
HRM Data Analysis
179
Troubleshooting
183
Log Archives
184
Hardware and Software Errors
184
HRM Troubleshooting
184
Error and Warning Messages
185
General Instrument Errors
185
Rotor-Gene Q Software Messages
187
Glossary
190
Technical Specifications
191
Environmental Conditions - Operating Conditions
191
Transportation Conditions
191
Storage Conditions
191
Mechanical Data and Hardware Features
191
Specifications (Hardware and Software)
192
Thermal Specifications
192
Optical Specifications
192
Appendix A - Legal
193
FCC Declaration
193
IEC en 61326 Compliance
194
Declaration of Conformity
195
Waste Electrical and Electronic Equipment (WEEE)
196
Liability Clause
197
Software License Agreement
198
Appendix B - Mathematical Techniques
201
Quantitation
201
Confidence Intervals for Calculated Concentrations
201
Confidence Intervals for CT Values
202
Ordering Information
203
Rotor-Gene Q MDX Products, Accessories, and Consumables
203
Document Revision History
206

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User Manuals: Qiagen 9002042 Real-time PCR Kit

Qiagen 9002042 User Manual (208 pages)

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