Qiagen QIAcuity 911000 User Manual page 262

Note: For a properly sealed plate, the plate seal covers the whole structure, and no bubbles or
strong depressions are visible, as this can also lead to bad filling of the wells.
8. The plate frame gives the option to mark the plate with a marker pen. Use the lane between
the plate edge and the printed letters (next to column 1) as well as the mirrored part (from
column 12 to the plate edge) only. Marking the plate seal above the wells is not
recommended as it might lead to bad filling of the wells.
Important: Do not mark the bottom side of the plate, as it is used to read fluorescence signals.
Note: Ensure that overlapping parts of the plate seal are turned down and well attached to the
plate frame and that the barcode is not covered. Do not apply pressure to the plate seal.
9. For the transport of the Nanoplate to the QIAcuity instrument, the plate should be held at the
side edges or on the tray horizontally. Make sure that the plate is transported to the QIAcuity
smoothly without shaking or turning of the plate, to ensure, that the reaction mix is at the
bottom of the input well.
10. The plate can now be used to start a run. For more information about starting a run on the
QIAcuity, see section Operating the QIAcuity Software Suite.
Note: Do not store the plate for more than 2 hours before the start of a run as this may lead to
pre-priming of the reaction mix resulting in reduced number of analyzable partitions.
11. You can store the plate at room temperature or at 4°C after the run for up to 1 week (Note:
Storage time may vary due to dye/probe stability, master mix and previous imaging
step/settings). You can re-image a plate up to 6 times (7 total imagings), see Setting up an
experiment for more information on how to re-cycle and re-image a plate.
QIAcuity User Manual 06/2022 262