Using The 100X Oil Immersion Objective - Olympus CX33 Instructions Manual

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Using the 100X oil immersion objective

· Apply the immersion oil specified by Olympus to the tip of the 100X oil immersion objective.
NOTE
Otherwise, the observed image is not in focus.
· Always use the immersion oil provided by Olympus.
Using the immersion oil which is not provided by Olympus cannot deliver the intended optical performance.
For observation with the 100X oil immersion objective, it is recommended to apply the immersion oil between
TIP
the tip of condenser lens and the specimen as well prior to observation.
· If you apply the immersion oil between the tip of condenser lens and the specimen, the condenser of this
microscope delivers the best optical performance in the observation with the 100X oil immersion objective.
If you do not apply the immersion oil, the image observed with the 100X oil immersion objective becomes
slightly dark.
· For observation with 4X to 40X objectives, it is not necessary to apply the immersion oil between the tip of
condenser lens and the specimen. (The brightness of the observed image is same regardless of whether
the immersion oil is applied or not.) However, for observation by changing from 4X to 40X objectives to the
100X oil immersion objective, it is recommended to apply the immersion oil between the tip of condenser
lens and the specimen in advance.
b
(When applying the immersion oil between the tip of condenser lens
1
and the specimen)
Apply the immersion oil at the tip of condenser lens a first, and
place the specimen.
a
2
Use the low magnification objective to bring the specimen into focus.
Apply the immersion oil on the observation position of the specimen
3 3
before engaging the 100X oil immersion objective into the light path.
Rotate the revolving nosepiece to engage the oil immersion objective
4
in the light path, and rotate the fine focusing knob to bring the
specimen into focus.
NOTE
To remove bubbles, rotate the revolving nosepiece slightly
If the immersion oil contains air bubbles, the image will
be degraded. Make sure that the oil is free of air bubbles.
to move the oil immersion objective back and forth for
once or twice.
CX33
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