Olympus BX53 Instructions Manual
  1. Manuals
  2. Brands
  3. Olympus Manuals
  4. Microscope
  5. BX53
  6. Instructions manual

Olympus BX53 Instructions Manual

System microscope
Hide thumbs Also See for BX53:
Table of Contents

Advertisement

Quick Links

See also: User Manual
INSTRUCTIONS
BX53
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscope Model BX53.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 5 5

Advertisement

Table of Contents
loading

Related Manuals for Olympus BX53

Summary of Contents for Olympus BX53

  • Page 1 BX53 SYSTEM MICROSCOPE This instruction manual is for the Olympus System Microscope Model BX53. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope.
  • Page 2 Refer to your local Olympus distributor in EU for return and/or collection systems available in your country. NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

  • Page 3: Table Of Contents

    BX53 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 10, “ASSEMBLY" (pages 34 to 37) carefully. IMPORTANT -- Be sure to read this section for safe use of the equipment. --...
  • Page 4 5-6 Immersion Objectives ................................. 26 Using Immersion Objectives ....26 5-7 Objectives with Correction Collar ..........................26 CAMERA RECORDING TROUBLESHOOTING GUIDE 28-30 SPECIFICATIONS 31,32 OPTICAL CHARACTERISTICS (UIS2 Series) ASSEMBLY 34-37 -- See this section for the replacement of the light bulb. -- LAMP HOUSING INSPECTION SHEET PROPER SELECTION OF THE POWER SUPPLY CORD ............

  • Page 5: Important

    UIS2 eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.

  • Page 6: Safety Symbols

    Rear panel position [Caution against high temperature] If a caution label is dirty or peeled off, contact Olympus for the replacement or other inquiry. Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. Also note that the microscope does not have a waterproof construction.

  • Page 7: Maintenance And Storage

    BX53 Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

  • Page 8: Module Nomenclature

    } The modules mentioned below show only the typical product names. As there are some products that are not mentioned but also applicable to this microscope, check the latest catalogues or consult Olympus. For the products marked “ * ”, also read their instruction manuals.

  • Page 9: Controls

    BX53 CONTROLS } If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 34 to 37). Light path selector knob (Page 23) Interpupillary distance adjustment scale (Page 11) Condenser height adjustment knob (Page 12) Diopter adjustment ring...
  • Page 10 Main switch (Page 1) I : ON : OFF Pre-focusing lever (Page 18) Pilot LED Fine adjustment knob Green lighting: Lamp bulb ON (Page 17) Red lighting: Auto OFF Detachable. Human sensor (Page 14) Light intensity preset switch (Page 13) Coarse adjustment knob (Page 17) Brightness adjustment knob...
  • Page 11 BX53 Low Magnification Condenser Swing-out Condenser U-LC U-SC3 Top lens Auxiliary lens mounting tool Top lens swing-out knob Aperture iris diaphragm ring (Page 12) Auxiliary lens for U-LC Condenser mount dovetail * The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 35)

  • Page 12: Flow Of Observation

    FLOW OF OBSERVATION } When the ECO mode is set, the halogen bulb turns OFF automatically in about 30 minutes the microscope becomes unattended. (Controls Used) (Page) Set the main switch to “ I ” (ON) and adjust @Main switch the brightness.
  • Page 13 BX53 } Copy the observation procedure pages on separate sheets and post it near your microscope.

  • Page 14: Simplified Observation Procedure

    SIMPLIFIED OBSERVATION PROCEDURE 4-1 Basic Operation (Until Observation of Specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each control, please read the description page specified below. Press the main switch of the microscope frame to “...

  • Page 15: Adjusting The Interpupillary Distance

    BX53 Rotate the knob to lower the stage. (Details: Page 17) Open the clamping lever of the slide holder and place the specimen slide. (Details: Page 19) Fig. 6 Rotate the revolving nosepiece to engage the 10X objective in the light path.

  • Page 16: Adjusting The Centering

    Adjusting the Centering Place the specimen. Rotate the revolving nosepiece to select the 10X objective. Rotate the knobs to bring the specimen into focus. Rotate the knob to raise the condenser to its upper limit. Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field of view.

  • Page 17: Using The Controls

    BX53 USING THE CONTROLS 5-1 Base Adjusting the Brightness (Fig. 11) Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter. } When the brightness adjustment knob @ is adjusted to the camera symbol marking, the brightness of the light becomes the daylight (color temperature of about 5500K).

  • Page 18: Using The Eco/Normal Switch

    Using the ECO/NORMAL Switch } The ECO mode helps prevent leaving the halogen bulb ON while the microscope is not used. 1. Press the ECO/NORMAL switch 3 to ON (so that the ECO indicator | lights in green). Hereafter, when the human sensor 5 of the microscope has not detected human motion, etc.
  • Page 19 BX53 Using Built-In Filters (Figs. 14 & 15) Use the filter knobs to engage or disengage the corresponding filters into or from the light path. Filter Type/Purpose ND6 (Neutral Density filter for light adjustment, transmittance 6%) ND25 (Neutral Density filter for light adjustment, transmittance...
  • Page 20 Mounting the Filter Cassette 1. Fully loosen the filter cassette clamping screw †. (Fig. 18) 2. Align the key ‡ on the bottom surface of the filter cassette with the posi- tioning slot 9 on the filter mount, then snap the filter cassette into place from above.

  • Page 21: Focusing Block

    BX53 5-2 Focusing Block Focusing Controls The focusing block consists of the controls listed in the following table, which also describes the functions of the controls. Name Function Coarse adjustment Moves the focus position widely. knob Fine adjustment dial Fine adjusts the focus position.

  • Page 22: Knob

    Replacing the Fine Adjustment Knob (Fig. 24) The fine adjustment knob has been attached on the right side CAUTION at the factory. } The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or X-/Y-axis knobs.

  • Page 23: Stage

    BX53 5-3 Stage Placing the Specimen } The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. } When observing very large specimens, remove the slide holder and place the specimen directly on the stage.

  • Page 24: Tension

    Adjusting the X- and Y-Axis Knob Tension (Fig. 30) 1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the tension and coun- terclockwise decreases it.

  • Page 25: Adjusting The X- And Y-Axis Knob 4 Rotating The Stage

    BX53 Rotating the Stage (Fig. 31) 1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. A click may be heard and felt during rotation. However, this is...

  • Page 26: Observation Tube

    5-4 Observation Tube Adjusting the Diopter When diopter adjustment rings are provided on both the left and right, it is possible to adjust the optimum vision for both the left and right eyes as described below. The diopter adjustment rings may be provided either on the eyepieces or the observation tube, and there is no problem even when the rings are not provided on both of them.

  • Page 27: Disk

    50% for camera * With the infrared trinocular tube, infrared observation up to 1000 nm is possible. For details, consult your Olympus representative. } The light path selector knob is located on the right side of the observation tube, but it can be removed and attached on the left side when the U-TTR is used.

  • Page 28: Adjusting The Tilt

    The intermediate attachments that can be combined with the CAUTION U-TTBI are limited. For details, please contact Olympus. With the U-TTLBI (Fig. 40) Fig. 40 The U-TTLBI is a lifting and telescopic binocular tube with the following adjustment capabilities.

  • Page 29: Using Eyepieces Incorporating A Micrometer

    BX53 Using Eyepieces Incorporating a Micrometer } When the eyepieces in use incorporate a micrometer, the accuracy of the left-right focusing adjustment (diopter adjustment) can be improved further. 1. Looking into the right eyepiece with your right eye, turn the top of the eyepiece @ so that the micrometer in the field of view looks sharpest (Fig.

  • Page 30: Immersion Objectives

    5-6 Immersion Objectives Be sure to use the provided Olympus Immersion oil. CAUTION Using Immersion Objectives (Fig. 43) 1. Focus on the specimen with objectives in the order of lower-power to higher-power ones. 2. Before engaging the immersion objective, place a drop of provided immersion oil onto the specimen at the area to be observed.

  • Page 31: Camera Recording

    BX53 CAMERA RECORDING } Use a trinocular observation tube such as the U-TR30-2 or U-SWTR-2 when shooting the video or digital camera picture of the microscope images. The trinocular tube accepts a camera adapter (certain TV adapters necessitate a camera mount adapter).

  • Page 32: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem...
  • Page 33 ) Visibility is poor. Immersion oil contains bubbles. Remove the bubbles. · Image is not poor. Recommended immersion oil is not Use the Olympus-designated immer- · Contrast is poor. used. sion oil. · Details are indistinct. Dirt/dust on specimen.
  • Page 34 Problem Cause Remedy Page e) Coarse adjustment will not go all Condenser holder is too low. Raise condenser holder. the way down. f ) Objective makes contact with speci- Specimen is mounted upside down. Mount specimen correctly. – men before focus is obtained. 4.

  • Page 35: Specifications

    BX53 SPECIFICATIONS Item Specification 1. Optical system UIS2/UIS (Universal Infinity System) optical system (featuring infinity correction) 2. Illumination Built-in transmitted Koehler illumination FN (Field Number): 26.5 (super-widefield compatible) 12V, 100W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 5761) (Average life time: Approximately 2,000 hr. when used as directed) Light intensity voltage range: 2 V or less to 12.0 V DC (continuous)
  • Page 36 Item Specification 7. Condenser Type U-LC U-AC2 U-SC3 U-AAC Low magnification Abbe Swing-out Achromat/ Aplanat N . A. 0.75 1.10 0.9 - 0.1 1.40 Aperture iris With numerical aperture scale diaphragm Objective 2X - 60X (for FN 4X (for FN 22 1.25X (for FN 22 10X - 100X (for range...

  • Page 37: Optical Characteristics (Uis2 Series)

    Mechanical tube length Oil: Oil immersion NOTE Iris: Iris diaphragm Refer to the latest catalogue or consult your local Olympus Field number (FN) representative for the updated information on the eyepieces Color band Cover glass thickness and objectives that can be combined with this microscope.

  • Page 38: Assembly -- See This Section For The Replacement Of The Light Bulb

    However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or remove this module).
  • Page 39 BX53 10-2 Detailed Assembly Procedures Attaching the Auxiliary Lens for the U-LC (Fig. 45) Condenser } It is not necessary to attach the auxiliary lens if the condenser in use is other than the U-LC. 1. Fit the mounting tool 2 (provided with the U-LC) into the groove on the collector lens for 100 W halogen bulb @ on the rear of the microscope, and turn the tool counterclockwise to remove the collector lens.
  • Page 40 Attaching the Eyepieces (Fig. 49) Gently insert the eyepieces all the way into the eyepiece sleeves. · When using the U-BI30-2 binocular tube, eyepieces with a po- CAUTION sitioning pin cannot be attached because the U-BI30-2 does not have the positioning notch. ·...
  • Page 41 1. Connect the lamp housing cable connector @ into the connector 2 on the rear of the microscope. Always use the power cord provided by Olympus. If no power CAUTION cord is provided with the microscope, please select the proper Fig.

  • Page 42: Lamp Housing Inspection Sheet

    { If there is any ( ) mark noted, immediately stop use of the product, and contact Olympus for detailed inspections or replace the lamp housing. { If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and contact Olympus for detailed inspections.

  • Page 43: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 44 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoni- Black-Red-Yellow Thread (Length zation Marking (May be located of color section in mm) Approval Organization on jacket or insulation of internal wiring) Black Yellow...
  • Page 45 MEMO...
  • Page 46 MEMO...
  • Page 48 Printed in Japan on April 00, 2010 M 010-01...

Table of Contents