Using The Oil Immersion Objective - Olympus BX53M Instructions Manual

4-14 Using the oil immersion objective

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4
Apply the specified oil (immersion oil) to the tip of the oil immersion
objective. Otherwise, the observation image can not be brought into
focus.
Always use the immersion oil made by Olympus. If you
NOTE
use the immersion oil other than those made by Olympus,
the correct optical performance cannot be exhibited.
1
Change the objective in order from the low magnification to the high
magnification to bring the sample into focus.
2
Before engaging the oil immersion objective in the light path, apply
a drop of the immersion oil on the observation area of the sample.
3
Turn the nosepiece, engage the oil immersion objective in the light
path and bring the sample into focus using the fine focusing knob.
· If bubbles are contained in the oil, the visibility of the image
NOTE
is deteriorated. Check that the bubbles are not contained
in the oil.
· To remove bubbles, turn the nosepiece slightly and move
the oil immersion objective back and forth once or twice.
4
After use, lower the stage, turn the nosepiece 90° and remove the
objective attached with the oil from the sample. Then, wipe off the
immersion oil completely from the tip of the objective and the tip of the
condenser lens using a piece of cleaning paper or the gauze slightly
moistened with the absolute alcohol. Wipe off the immersion oil on
the sample in the same way.
If lenses or samples are left keeping the immersion oil
NOTE
attached, the oil adheres and the correct observation may
not be performed.
Follow the cautions indicated in the label of the immersion
CAUTION
oil.
BX53M
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