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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 1 1 Olym mpus BX X53 Digit tal Micro oscope User Gu uide A. Sy ystem Start‐u...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 2 2 5. Re emote contro oller box co ontrols the se election of im maging mode ( (i.e. BF or Flu uorescence (F L)). Use the M Mirror ...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 3 3 5. S Select a came era from the C Camera list : XM10 mono ochrome cam mera for fluor rescence or D DP26 color cam mera for ...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 4 4 E. Fl uorescence I mage Acquis sition 1. C Click the FL bu utton on th he remote co ontroller box t to use mercu ry arc lamp li...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 5 5 3. Le et’s take mult ticolor fluores scence image e acquisition w with an exam ple stained w with DAPI, FIT TC, and T TxRed fluorop phores. ...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 6 6 F. Im mage Process ing 1. O nce you save the original f file, make a d uplicate if it i is necessary t to make any c changes in th e image for p...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 7 7 3. T To save some overlaid imag ges from a m ultichannel im mage you just t made as abo ove, there ar e three ways (note: these ...
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 8 8 F. Ac cquisition of Z Z‐stack (z‐ser ies) 1. A z‐stack image e contains mu ultiple frames s acquired at different foc us positions.
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MW Microscop py Laboratory y O l y m p u s B X X 5 3 U s e r G u i d e | 9 9 5. Yo ou can projec ct all the z‐sta ack frames int to a single 2D D image by sel lecting Proces ss/Projection ns/Maximum m Z command ...
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