Tighten the fitting finger-tight, plus as much as an additional 1/6-turn using a 1/4-inch open-end
wrench.
Reinstalled tightening
2.7 Sample preparation
Micro-scale UPLC analysis places some additional restrictions on sample preparation.
Particle-free mobile phase solvents and sample solutions are essential for micro-scale UPLC
analysis. See
General guidelines for a microscale system
for recommendations on choosing and
handling solvents.
November 26, 2019, 715003588 Revision C
Page 47