Waters ACQUITY UPLC M-Class System Manual page 9

1.2.9 Software............................................................................................................................... 19
1.2.10 Options .............................................................................................................................. 19
1.3 Additional information.................................................................................................................... 20
2 Performance optimization........................................................................................22
2.1 Best practices to improve performance......................................................................................... 22
2.1.1 Autozeroing the flow transducers ........................................................................................ 22
2.1.2 Flow ramping ....................................................................................................................... 22
2.1.3 Flow rate on the B flow controller ....................................................................................... 23
2.1.4 Initial gradient hold............................................................................................................... 23
2.1.5 Trapping flow rate and trapping composition....................................................................... 23
2.1.6 Re-equilibration of column at the end of analysis ............................................................... 24
2.1.7 System equilibration after auto zero .................................................................................... 24
2.1.8 Re-equilibration between methods ..................................................................................... 24
2.1.9 Temperature sensitivity ........................................................................................................ 24
2.2 General guidelines for a microscale system ................................................................................. 25
2.2.1 Required solvents ................................................................................................................ 25
2.3 Dispersion ..................................................................................................................................... 26
2.4 Carryover ...................................................................................................................................... 26
2.5 Contamination ............................................................................................................................... 27
2.5.1 Contamination when operating with neutral or basic mobile phases................................... 28
2.6 Preventing leaks............................................................................................................................ 29
2.6.1 Installing tubing and fittings ................................................................................................. 29
2.7 Sample preparation....................................................................................................................... 47
3 Preparation and operation ......................................................................................48
3.1 Preparing the system .................................................................................................................... 48
3.1.1 Powering-on the system hardware ...................................................................................... 48
3.1.2 Configuring the system software ......................................................................................... 49
3.1.3 Monitoring LEDs .................................................................................................................. 49
3.1.4 Power LED........................................................................................................................... 49
3.1.5 Status LEDs......................................................................................................................... 49
3.2 Starting the instrument console..................................................................................................... 51
3.3 Instrument control panels.............................................................................................................. 52
3.3.1 Solvent manager control panels .......................................................................................... 52
November 26, 2019, 715003588 Revision C
Page ix