System Recommendations; Solvent Manager Recommendations - Waters ACQUITY UPLC M-Class System Manual

D.2.4.7 Conditioning following neutral or basic mobile phase operation
To avoid bacterial growth, when powering-down the system, or when injections are stopped for >4
hours, flush the system with 100% neutral deionized water followed by a 100% organic mobile
phase. This removes neutral or basic mobile phase from the solvent manager, sample manager,
and solvent lines.
If you are using iKey devices, flush them after each use with 100% organic mobile phase.
D.3 System recommendations
Notice:
capillary lines, ensure that solvents consist of at least a 10% organic component, such
as acetonitrile or methanol, and completely replace the mobile phase daily. Pure
aqueous solvents must not remain in a powered-down system because they serve as a
substrate for microbial growth.
• Contact Waters for recommended system cleaning and flushing procedures.
• Contact your Waters sales representative or local technical support organization to determine
the typical chromatography conditions or operating parameters suitable for ACQUITY UPLC
M-Class system modules.
• Consider using chloroform, methylene chloride, halogenated solvents, or toluene in weak
dilutions (less than 10% volume) as additives, modifiers, or sample diluents.
• Waters does not recommend methanesulfonic acid for use in ACQUITY UPLC M-Class
systems.
• Always use properly cleaned and maintained glassware.
Notice:
cart or instrument modules, or when adding or changing reagents.
D.3.1 Solvent manager recommendations
• The seal wash system must never run dry, particularly during separations that use a polar
mobile phase.
• For reversed-phase applications, use aqueous seal wash solutions with a weak organic
component (for example, 1:9 methanol/water).
• Do not use nonvolatile buffers as seal wash solvents.
• Ensure that the mobile phase is completely soluble in and compatible with all of the solvents
in use on the system.
To prevent the proliferation of microbial colonies that clog system filters and
To prevent injury, always remove the solvent bottles when moving the micro
November 26, 2019, 715003588 Revision C
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