Pure Water Is Required; Solvent Recommendations; Recommended Solvents - Waters ACQUITY UPLC M-Class System Manual

Store mobile phases in borosilicate glass reservoirs type 1, class A
stained glassware to inhibit microbial growth. Use aluminum foil or Waters caps to cover the
reservoirs.
D.1.4 Pure water is required
Use water only from a high-quality water purification system. If the water system does not deliver
filtered water, filter the water through a 0.2-µm membrane filter.
Recommendation:
Notice:
capillary lines, ensure that solvents consist of at least a 10% organic component, such
as acetonitrile or methanol, and completely replace the mobile phase daily. Pure
aqueous solvents must not remain in a powered-down system because they serve as a
substrate for microbial growth.
D.1.4.1 Using buffers
Adjust the pH of aqueous buffers. Filter them to remove insoluble material, and then blend them
with appropriate organic modifiers. After you use a buffer, flush it from the pump by wet priming
using at least five system volumes of HPLC-grade, distilled or deionized water.
To prevent microbial growth during shutdowns lasting longer than 24 hours, flush the pump and
solvent lines with a solution of organic solvent/water in which the organic component is greater
than 20%.
Warning:
incompatible buffers, consult the instrument's accompanying documentation to
determine what buffers are compatible.
Tip:
To avoid salt precipitation, nonvolatile buffer concentrations must not exceed 100 mM.
D.2 Solvent recommendations
This section lists solvents recommended for the ACQUITY UPLC M-Class System. Contact
Waters Customer Service to determine whether you can use solvents that do not appear in the
list without adversely affecting performance of system modules or the system itself.
D.2.1 Recommended solvents
• Acetonitrile
• Acetonitrile/water mixtures
• Isopropanol
Use LC/MS-quality (or better) water.
To prevent the proliferation of microbial colonies that clog system filters and
To avoid damaging a mass spectrometer by unintentionally introducing
November 26, 2019, 715003588 Revision C
Page 103
2 3
or type 3.3 . Use brown-