Acquity Uplc M-Class System; Configurations Of System Modules - Waters ACQUITY UPLC M-Class System Manual

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ACQUITY UPLC M-Class system

Waters designed the ACQUITY UPLC M-Class system to perform a range of chromatographic
separations from nano-scale to micro-scale. With a maximum operating pressure of 103,421 kPa
(1034 bar, 15,000 psi), an analytical flow rate to 100 µL/min, and a trapping flow rate to 1 mL/min,
the system produces optimal linear velocity for columns that range in internal diameter from 75
µm to 1.0 mm.
The system is compatible with Waters mass spectrometers and optical detectors, supporting a
range of column diameters. Options include:
• NanoFlow ESI and NanoLockSpray sources for Waters time-of-flight and tandem MS, and
flow rate ranges from 200 nL/min to 2,000 nL/min.
• Micro-scale ESI probe for use in the ZSpray API and LockSpray dual electrospray ion sources
for flow rate ranges from 5 µL/min to100 µL/min.
• A 100-nL, light-guided flow cell for columns ranging from 300 µm to 1.0 mm ID with the
ACQUITY UPLC PDA and TUV detectors.
• A 10-nL, transverse-illuminated flow cell for nano-scale separations with the ACQUITY UPLC
TUV detector.
ACQUITY UPLC M-Class systems that include mass spectrometry, optical detection, or both can
analyze compounds found in low concentrations amid complex matrices, quantifying peptides, or
metabolites (when used with a tandem mass spectrometer).

1.1 Configurations of system modules

This section shows examples of the ACQUITY UPLC M-Class system stack consisting of the core
modules. Your system configuration may differ according to the number and types of modules it
contains. For example, if your system includes a mass spectrometer, the system modules may be
arranged in two separate stacks.
See also:
For information on the ACQUITY UPLC M-Class systems with HDX technology and
the ACQUITY UPLC M-Class systems with HDX Automation, refer to the ACQUITY UPLC M-
Class with HDX Technology System Guide on the documentation CD.
November 26, 2019, 715003588 Revision C
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