Waters ACQUITY UPLC M-Class System Manual page 69

4.3.2.6 Late eluting peaks fail
Suspected cause
Inappropriate gradient method
Incorrect trapping conditions
Incorrect sample preparation
4.3.2.7 Missing peaks
Suspected cause
Detector problems
No solvent flow
Leak in the solvent path
No spray
Incorrect vial position
Injector not injecting
4.3.2.8 Peak shape and area issues
Suspected cause
Incorrect detector settings
Injection volume/sample concentration too
high; disrupting column equilibration
Possible solution
Use the correct gradient method.
Use the standard trapping conditions.
Prepare the sample correctly.
Possible solution
Inspect all fittings and replace as necessary.
See also:
Installation and Maintenance Guide on the
documentation CD.
Ensure that the vial is in the correct position.
Possible solution
See also:
and maintenance guide on the documentation
CD and the online Help.
November 26, 2019, 715003588 Revision C
Page 69
• Confirm that the system is plumbed into the
detector with no leaks.
• Verify that the detector is operational.
• Confirm that there is sufficient solvent in
the bottles.
• Ensure that the solvent flow rate is >0.0,
and that the system pressure is normal.
The Universal NanoFlow Sprayer
• Prime the sample syringe.
• Consult the Console online Help.
The applicable detector overview
• Reduce the injection volume.
• Dilute the sample.