Waters ACQUITY UPLC M-Class System Manual page 108

5. Prime the seal wash line with 70% isopropyl alcohol for 10 minutes or more.
6. Place lines A1, A2, B1, and B2 in the solution.
Tip:
If you will not use A2 and B2 lines for high pH solvent, you can place only A1 and B1
in the solution, skip step 9 and step 12, and use A1 and B1 for step 10. Likewise, if you will
not use A1 and B1 lines for high pH solvent, you can place only A2 and B2 in the solution
and skip step 8 and step 13.
7. Prime the A1 and B1 lines for 10 minutes.
Note:
The prime operation changes the vent valve to vent to waste. It does not allow
eluent to go to the flow control module.
8. Prime the A2 and B2 lines for 10 minutes.
9. Remove the columns, and then flow the 70% isopropyl alcohol through the A2 and B2 lines
at 6 μL/min for a minimum of two hours.
Recommendation:
overpressurization.
See also:
10. Prime the seal wash line with seal wash solution for 10 minutes or more to remove the
isopropyl alcohol.
11. Remove all lines from the 70% isopropyl alcohol and place them in the solvents you will
use for the assay.
Restriction:
bottom of the bottle.
12. Prime the A2 and B2 lines with the assay mobile phase for five minutes.
Note:
Priming will retain 70% isopropanol in the flow control module.
13. Prime the A1 and B1 lines with the assay mobile phase for five minutes.
14. Remove all the 70% isopropyl alcohol from the system by doing one of the following:
• In a µBSM or ASM, you can gradually increase the flow rate. Starting at 1 μL/min,
increase the flow in 0.5 to 1.0 μL/min increments. Move to the next increment only
when the pressure stops increasing. There is no upper flow rate limit, but you cannot
exceed the system pressure limit.
• In a µBSM, you can use the set pressure diagnostic. When the pressure stabilizes, you
can assume that all the isopropyl alcohol is flushed from the system.
D.2.4.5.2 Flushing the high pH fluidic path
You may also want to flush the fluidic path that is supplied by the solvent manager delivering the
high-pH solvent. This occurs concurrently with the solvent manager cleaning. Refer to the
following diagrams for setting up this fluidic path so that it flows to waste. Prior to running the
cleaning procedure, remove all separation devices from this fluidic path and replace them with
unions. In an ionKey/MS system, replace the iKey Analytical device with the iKey Infusion device.
Run the system at a constant system pressure to prevent
To run at constant pressure in a µBSM, use the set pressure diagnostic.
Do not allow solvent lines in neutral or basic mobile phases to rest on the
November 26, 2019, 715003588 Revision C
Page 108