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2.3.2
Equilibrating the system
Equilibrating the system involves setting the conditions for the method you plan to run for your
analysis. For example, setting the flow rate, solvent composition, column temperature, ABPR
pressure and other such conditions.
See:
The ACQUITY UPC
for details about priming and equilibrating the system. Also see the ACQUITY online Help.
2.4
Observing the sample-injection sequence
The following diagrams illustrate the sample set up and injection sequence in an ACQUITY UPC
system.
Figure 2–3:
Waste
Sample
Liquid mobile phase (CO
Gas mobile phase (CO
Air gap
At the start of an injection, the injection valve is in the "inject" position (from the previous injection).
The SM-FL signals the auxiliary valve to turn 60° to the "load" position, to enable the connecting
tubes from the two valves to vent to atmosphere (including the sample loop on the injection valve).
2
Binary Solvent Manager Operator's Overview and Maintenance Guide
Step 1: SM-FL runs internal setup tests and decompresses the sample
loop
Auxiliary valve (load position)
To column
From pump
Sample loop
Injection valve (inject position)
)
2
)
2
November 16, 2016, 715004521 Rev. C
Waste
Metering syringe
Buffer volume
Sample
Weak wash
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2
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