Sample Preparation And Analysis Flow Paths; Trapping; Analysis - Waters TRIZAIC UPLC System Manual

Sample preparation and analysis flow paths

Trapping

The flow path connects the nanoBSM to the injection valve on the
nanoACQUITY sample manager. Sample flow continues from the injection
valve to the nanoTile's trap channel in the TRIZAIC UPLC source, and then to
a waste line on the valve in the heating and trapping module.

Analysis

When trapping is completed, the gradient flows through the nanoTile's trap
and analytical channels to elute and separate the analytes. The analytes then
flow through the electrospray emitter, and into the mass spectrometer, for
analysis.
Flow path for single-pump trapping:
nanoACQUITY sample manager
Needle
Syringe
1-6 TRIZAIC UPLC System Capabilities
TRIZAIC UPLC source
To waste
nanoBSM
NanoTile device
Trap column
Analytical column
HTM trap valve
Mass spectrometer