Waters ACQUITY UPLC M-Class System Manual page 72

Suspected cause
Needle weak wash solvent is too high in
organic.
In-line filter, column inlet, or connecting tubing
is partially blocked.
Column or columns are contaminated.
Column or columns are degraded, forming a
void.
Trap column is degraded.
4.3.2.13 Tailing peaks with a normal retention time
Suspected cause
Fitting or interconnection problem
Bad consumables
4.3.2.14 Tailing peaks with increased retention time
Suspected cause
Leak in the system
Possible solution
Ensure that the weak needle wash solution is
similar to the initial mobile phase.
Inspect these components for particle build-up.
Clean or replace.
Replace columns.
See also:
use guide on the documentation CD.
Verify column performance. If efficiency is low,
replace it.
Possible solution
Inspect sprayer and emitter plumbing
connections.
See also:
Installation and Maintenance Guide on the
documentation CD.
Perform a systematic replacement of
consumables beginning with the analytical
column.
Possible solution
If the problem persists, perform the set-
pressure diagnostic test to inspect for system
leaks.
See also:
information.
November 26, 2019, 715003588 Revision C
Page 72
The appropriate column care and
• Remove trap column and perform a direct
injection.
• If results are normal, replace the column
and perform a set pressure diagnostic.
The Universal NanoFlow Sprayer
The online Help for more