Waters ACQUITY UPLC M-Class System Manual page 112

3.
4.
2. Insert the strong and weak wash lines in a bottle of 70% LC/MS-grade isopropanol 30%
water solution.
3. Prime the sample manager for at least six cycles, as follows:
a. In the console system tree, select the sample manager.
b. From the Control menu, select Prime syringes.
c. In the Prime Syringes dialog box, select Sample syringe and wash syringes and
specify 6 or more cycles in the Number of cycles field.
4. After priming completes, insert the strong and weak wash lines into bottles of the strong
and weak wash solvents that are appropriate for your application.
5. Prime the sample manager for least six cycles (see step 3).
Note:
When the system is not running, do not leave neutral or high pH solvent in the sample
manager. Following operation, prime the sample manager with acidic or organic solvents for at
least six cycles as described in this section.
D.2.4.6 Replacing bottle sinkers at least monthly
Bacteria from different solvents can seed bacterial growth in the crevices of the sintered metal of
bottle sinkers. To deter growth of bacteria on the sintered metal, replace bottle sinkers at least
monthly. If problems arise with neutral or basic mobile phase operation, replace the sinkers more
frequently.
Figure D–6: SEM image showing bacterial growth on a bottle sinker when exposed to
high-pH mobile phase
In the Injection Valve dialog box, click Load.
Click Control to end the editing session.
November 26, 2019, 715003588 Revision C
Page 112