Column Technology - Waters ACQUITY UPLC M-Class System Manual

1.2.7.1 Optical detection
The ACQUITY UPLC PDA and TUV are optical detectors that detect and quantify concentrations
of sample analyte. Low-dispersion flow cells developed with Teflon-AF are available for use with
microscale 1.0-mm and 300-mm ID columns.
See also:
The ACQUITY UPLC M-Class System documentation CD for the following
documents:
• ACQUITY UPLC TUV Detector Operator's Overview and Maintenance Guide
• ACQUITY UPLC Photodiode Array and e λ Photodiode Array Detector Operator's Overview
and Maintenance Guide
1.2.7.2 Mass detection
The ACQUITY UPLC M-Class system maximizes peak capacity, extending the dynamic range of
sample analysis. The ACQUITY UPLC M-Class system is frequently used with mass
spectrometers, whether analyzing complex samples in wide-ranging matrices with a high-
resolution MS, or quantifying peptides, metabolites, or other targeted experiments with tandem
MS. Engineered for the lowest dispersion, including an improved ESI micro-probe design (and
with an extended pressure envelope) complex separations are accelerated without compromising
the chromatography.
See also:
The Waters Driver Pack 2019 Release 2 Installation and Configuration Guide
(715006278) for compatible mass spectrometers and the associated MassLynx software change
note (SCN).

1.2.8 Column technology

Configure columns with the TVM using no valves for direct injection, or one or two valves (when
used with two-dimensional applications, for example).
1.2.8.1 Columns
The TVM supports nano-scale (75-mm ID), capillary-scale (300-mm ID), and micro-scale (1.0-mm
ID) separations. You can choose among these options when selecting columns for the system:
• Nanoscale analytical columns:
• 75 µm to 150-µm ID
• 50 mm to 250 mm length
• Capillary-scale analytical columns:
• 300 µm-ID
• 50 mm to 150 mm length
• Microscale analytical columns:
November 26, 2019, 715003588 Revision C
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