Developing Methods; Sample Preparation; Particulates; Matching Sample Diluents - Waters ACQUITY UPLC H-Class Series System Manual

Figure 2–10: Screwing cap nut onto fitting
#1 knurled compression fitting
#2 locking cap nut

2.6 Developing methods

See also:
For information about method development and validation, consult the Auto•Blend
Plus Technology for Ion Exchange, Size Exclusion, and Reversed-phase Chromatography
documentation, included on the system documentation media.
For the greatest flexibility in method development, Waters recommends configuring the system
with the column manager and optional Auxillary column managers as well as installing the
optional 6-solvent select valve in the QSM. Using the standard Auto•Blend Plus technology will
automate the preparation of any pH specific mobile phase from pure solvents for easier method
development.

2.7 Sample preparation

2.7.1 Particulates

Waters recommends filtering all samples with particulates through a 0.2 µm sample filter or
installing a column pre-filter. The small column frit size (0.2 µm) can become blocked more easily
than larger HPLC column frits (2.0 µm). As a result, particle-free mobile phase solvents and
sample solutions are essential for UPLC analysis. See
on choosing and handling solvents.

2.7.2 Matching sample diluents

When you use the sample manager's auto-dilution option, the purge solvent serves as the sample
diluent. Ensure that your sample solution is soluble and miscible in your chosen purge solvent.
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General guidelines for recommendations