Sample Preparation; Particulates; Matching Sample Diluents - Waters ACQUITY UPLC I-Class System Manual

the intake cycle. Leaks at high pressure fittings (downstream of the i
can leak solvent but do not introduce air.
To prevent leaks, follow Waters' recommendations for the proper tightening of
system fittings. Note specifically that different techniques apply to
retightening fittings versus installing them for the first time.
See also:
• The user documentation for the specific system modules for more details
on leak detection.
• The ACQUITY UPLC Column Compartments Operator's Overview and
Maintenance Information for details on connecting active preheaters.

Sample preparation

UPLC analysis places some additional restrictions on sample preparation.

Particulates

The small column frit size (0.2 μm) can become blocked more easily than
larger HPLC column frits (2.0 μm). Particle-free mobile phase solvents and
sample solutions are therefore essential for UPLC analysis. See
guidelines" on page 2-2
solvents.

Matching sample diluents

When you use the auto-dilution option on the SM-FTN, the purge solvent
serves as the sample diluent. Ensure that your sample solution is soluble and
miscible in your chosen purge solvent.
Caution:
sample diluents match the initial conditions of your gradient.
2-6 Optimizing Performance
for recommendations on choosing and handling
To avoid broad peaks and poor peak shape, ensure that your
2
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