Revision History - Biotek Synergy HT Operator's Manual

Multi-mode microplate reader
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Revision History

Rev
Date
Changes
A
4/2002
First issue
B
8/2002
Added Time-Resolved Mode
C
8/2003
Added Dual Fluid Dispense Feature
Ch. 1, Introduction: Updated specifications, accessories, and technical support.
Ch. 2, Instrument Description: Updated component descriptions and added
drawings.
Ch. 3, Installation: Revised Dispenser Module setup instructions and KC4 launch
procedure. Revised unpacking/repackaging instructions.
Added new Chapter 4, "Getting Started With KC4."
Ch. 5, Performance Verification/Qualification Tests (formerly Chapter 4): Revised
test procedures.
Reformatted Appx A, Decontamination, and Appendix B, Computer Control.
Updated Appx C, Error Codes. Renamed Appx D, Microplate Location Dimensions
to "Instrument Dimensions."
D
12/2003
Preface: Updated safety symbols and text (p. ix and x). Updated Intended Use
Statement (p. xi). Revised Warranty (p. xii).
Chapter 1: Modified Introduction (p. 1-3). Updated list of optional accessories (p.
1-4). Revised absorbance reading speed information (p. 1-5). Clarified
fluorescence specifications (p. 1-8 and 1-9). Added specifications for injector
model (p. 1-10).
Chapter 2: Clarified description of external and internal components of the
injector model and updated drawings. Moved procedure for replacing the lamp
assembly to Ch. 6.
Chapter 5: Added note regarding the availability of the Installation-Operational-
Performance (IQ-OQ-PQ) package (PN 7090521) (p. 5-2). Updated liquid test
procedures.
Added new Chapter 6, Maintenance and Troubleshooting, which includes: Sample
reproducible page from maintenance logbook; Procedures for maintenance and
routine cleaning; Instructions for changing injector positions.
Updated decontamination procedure (Appendix A). Modified Appendix B,
Computer Control. Corrected Appendix C, Error Codes. Changed "Dispenser
Module" to "Dispense Module" throughout.
E
2/2004
Chapter 1, Introduction: Removed reference to "NB" version (p. 1-3). Added
specifications to reflect the use of an additional PMT type (R4220PHA) to
Hardware Features (p. 1-3). Moved filter plug (7082073) from Optional
Accessories list to Package Contents (p. 1-4). Added required specifications for
microplates used in "Luminescence" mode
(p. 1-5). Added "6- to 96-well plates" and "fluorescence and luminescence read
modes" to Injector Model features (p. 1-10). Removed "option" from reference to
Incubation specifications (p. 1-11).
Chapter 3, Installation: Removed references to "NB" version from description of
reader model options (Setting Communication Parameters in KC4, p. 3-12).
Chapter 5, Performance Verification/Qualification Tests: Updated Figure 5-1,
Sample System Test (p. 5-6 and 5-7).
Updated Appendix C, Error Codes.
Synergy HT Operator's Manual
vii
Revision History
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Questions and answers

Luqman Babeer
April 25, 2025

I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you

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1 comments:
Mr. Anderson
May 12, 2025

To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:

1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.

If the issue persists, contact BioTek’s Technical Assistance Center.

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