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146
Appendix C: Error Codes
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Code
power supply PCB sent the wrong voltage request to the lamp power supply.
Scenario 4 (Reference channel):
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB intermittently failed.
• Missed flashes or an erratic flash lamp.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Voltage to lamp has increased due to failure of the lamp power supply, or motor power
supply PCB sent the wrong voltage request to the lamp power supply.
0502
Light beam saturated (too much light). Air measurement channel reading reached
65535 or PMT Relative Fluorescing Units (RFU) reached FFFF (99999).
This error can indicate one of the following scenarios:
• (1) Prior to a fluorescence read or during a system test, the PMT is tested for operation; the
gain is set at 102. Is the PMT operating properly?
• (2) Filter 2 in absorbance mode has saturated the reference channel.
Probable Causes:
Scenario 1 (Fluorescence or System test):
• The PMT is defective.
• The connector from the PMT base to the analog board was not built correctly. The
ground/shield is not grounding properly.
• The PMT base is defective.
• The PMT analog PCB is defective.
Scenario 2 (Reference channel):
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB has intermittently failed.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Missed flashes or an erratic flash lamp.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.
0503
Light beam saturated (too much light). Air reading reached 65535.
This error indicates that filter 3 in absorbance mode has saturated the reference channel.
Probable Causes:
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB intermittently failed.
• Missed flashes or an erratic flash lamp.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.
Description and Probable Causes:
BioTek Instruments, Inc.
- Quick Links:
- Error Codes
- Other Errors (2A00-4000)
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Table of Contents
Troubleshooting
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Do you have a question about the Synergy HT and is the answer not in the manual?
Questions and answers
I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you
To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:
1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.
If the issue persists, contact BioTek’s Technical Assistance Center.
This answer is automatically generated