Decontaminate The Fluid Lines - Biotek Synergy HT Operator's Manual

128
Appendix A: Decontamination
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8. Reassemble the instrument as necessary.
9. If the dispense module is installed, detach the outlet tubes from the rear panel
of the instrument. If it is not installed, attach just the dispense module's
communication cable to the instrument. Remove the supply bottles and their
holders.
10. Perform the procedures described below through page 130 to decontaminate
the fluid lines in the dispense module, the internal tubing and injector heads,
and the tip priming trough and priming plate.

Decontaminate the Fluid Lines

1. Place a beaker with 20 mL of 0.5% sodium hypochlorite solution or 70%
isopropyl alcohol near
Place the SYRINGE 1
2.
3. If you have not already done so, detach the dispense module's
from the instrument's rear panel. Place the ends of the outlet tubes in an empty
beaker and set the beaker on the work surface.
4. Launch Gen5, select
Dispenser
5. Select Dispenser 1
dispense
Place the
6.
presence).
7. Run two
8. Pause for
9. Remove the
10. From the
11. Run one
12. Empty
Important! If sodium hypochlorite (bleach) was used, perform
13.
Lines
Otherwise, (or after performing the Rinse procedure), repeat steps 1–13 for
SYRINGE 2
SYRINGE 1
inlet tube
System > Reader/Instrument Control,
tab.
, enter a
Rate.
on the carrier (it is not used, but the reader requires its
priming plate
prime cycles, for a total of 10000 µL.
20 to 30 minutes
inlet tube from the beaker of disinfectant solution.
Reader Control dialog, change the
prime cycle, to flush the disinfectant out of the fluid lines.
the beaker containing the outlet tubes. Put the tubes back in.
on the next page.
/ Dispenser 2
on the dispense module.
in the beaker.
Volume of 5000 µL , and keep the default
to allow the solution to disinfect the tubing.
Volume
.
outlet tubes
and click the
to 1000 µL.
Rinse the Fluid
BioTek Instruments, Inc.