Biotek Synergy HT Operator's Manual page 179

Multi-mode microplate reader
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flash off.
• The reference channel failed < 100 or the Dark value has changed more than 10% from the
last self-test data during a read or blank read with the flash on.
Probable Causes:
• Absorbance analog PCB or reference channel analog PCB is defective.
• Cable between reference channel and analog PCB is defective or disconnected.
• Reference channel photodetector is defective or the optic spray is damaged.
• A faulty analog PCB or faulty internal grounding may cause internal electronic noise.
• There may be an ambient light leak. Make sure the plate carrier door and the front hinged
door are properly closed.
• Electrical noise may be penetrating the measurement chamber. The bottom and top
shrouds are part of the electrical shielding. Verify that the shrouds are installed and are
properly fastened.
• Order-sorting filter wheel is jammed and not aligning the filter wheel to block the light
path, or the filter is degraded and not passing enough light energy.
0910
The absorbance measurement channel Dark current value failed
See "Dark" on the system test. See criteria in text below.
This error can indicate one of the following scenarios:
• The measurement channel failed during a read or spectral scan for one of the following
reasons:
• The measurement channel failed < 100 or > 20000 during filter calibration or spectral scan
with the flash on.
• The measurement channel failed < 100 during filter calibration or spectral scan with the
flash off.
Probable Causes:
If failed < 100:
• Absorbance analog PCB or measurement channel analog PCB is defective.
• Shielding of the cable between the measurement channel and analog PCB is defective or
disconnected.
• Measurement channel photodetector is defective.
If failed > 20000:
• Measurement channel photodetector is defective.
• A faulty analog PCB or faulty internal grounding may cause internal electronic noise.
• There may be an ambient light leak. Make sure the plate carrier door and the front hinged
door are properly closed.
• Electrical noise may be penetrating the measurement chamber. The bottom and top
shrouds are part of the electrical shielding. Verify that the shrouds are installed and are
properly fastened.
• Order-sorting filter wheel is jammed, not aligning the filter wheel to block the light path, or
the filter is degraded and is not passing enough light energy.
The absorbance measurement channel Dark current value failed
0911 - 0916
See "Dark" on the system test. See criteria in text below.
The last number is the lambda table position number. This error can indicate one of the
following scenarios:
• The measurement channel failed < 100 during optic test with the flash on.
Synergy HT Operator's Manual
Description and Probable Causes:
Dark value was < 100 during a spectral scan using 8 flashes and 8 resets during
-
sweep mode.
Dark value was < 100 during a spectral scan using 8 flashes and the number of
-
resets saves for that wavelength during normal and rapid mode.
153
Error Codes |

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