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Biotek Synergy LX User Manual

Multi-mode microplate reader
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Synergy LX
Multi-Mode Microplate Reader
User Manual
ERRATA NOTICE: This document contains references to BioTek.
Please note that BioTek is now Agilent. For more information,
go to www.agilent.com/lifesciences/biotek.

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Summary of Contents for Biotek Synergy LX

  • Page 1 Synergy LX Multi-Mode Microplate Reader User Manual ERRATA NOTICE: This document contains references to BioTek. Please note that BioTek is now Agilent. For more information, go to www.agilent.com/lifesciences/biotek.
  • Page 2 Synergy LX Multi-Mode Microplate Reader User Manual ERRATA NOTICE: This document Document part number 1501000N, revision A contains references to BioTek. BioTek Instruments, Inc. Please note that BioTek is now Agilent. For more information, go to December 2021 www.agilent.com/lifesciences/biotek.

  • Page 3: Table Of Contents

    5. Install the Power Supply 6. Turn on the Reader and Run the Power-Up System Test 7. (Gen5 control only) Start Gen5 and Test Communication Operational/Performance Qualification Repackaging and Shipping Instructions Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 4 Plate Shaker Test Absorbance Testing Fluorescence Testing Luminescence Test Instrument Qualification Procedures Overview IQ/OQ/PQ Description Recommended Qualification Schedule System Test Absorbance Plate Test Absorbance Liquid Tests Fluorescence Plate Tests Fluorescence Liquid Tests Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 5 Absorbance Specifications Fluorescence Specifications Luminescence Specifications Sample Reports Sample System Report Sample Absorbance Test Plate Report Sample Assay Results Error Conditions Overview Error Codes Safety Information Safety Notices In This Book Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 6: Notices

    BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 7: Contact Information

    Instrument service and repair is available worldwide at one of our international service centers and in the field at your location. UK Responsible Person (UKRP) Agilent LD UK Ltd 5500 Lakeside Cheadle Royal Business Park Cheadle, Cheshire SK8 3GR Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 8: Intended Use Statement

    Failure to conduct Quality Control checks could result in erroneous test data. Warranty and Product Registration Review the warranty information that shipped with your product. Register your product to ensure you receive important information updates about the products you have purchased. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 9: Safety Notices

    Service. Only qualified technical personnel should perform service procedures on internal components. Power Supply. Use only the power supply shipped with the instrument, and operate it within the range of line voltages listed on it. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 10 Turn off the reader and allow the bulb to cool for at least 15 minutes before attempting to replace it. Accessories. Only accessories that meet the manufacturer's specifications shall be used with the instrument. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 11 Failure to conduct quality control checks could result in erroneous test data. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 12 Use of labware other than described in this document can result in positioning errors during program execution. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 13: Symbols

    Precaución, consulte las instrucciones de uso para obtener información importante, como advertencias y precauciones Warning; Biological hazard Waarschuwing; biologisch gevaar Avertissement : Risque biologique Warnung; biologische Gefahr Avvertenza, rischio biologico Advertencia: peligro biológico Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 14 Warnung; Quetschgefahr Avvertenza, rischio di pizzicamento Advertencia: peligro de atrapamiento Warning; Hot surface Waarschuwing; heet oppervlak Avertissement : surface chaude Warnung; heiße Oberfläche Avvertenza, superficie molto calda Advertencia: superficie caliente Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 15 2012/19/EU, sui "rifiuti di apparecchiature elettriche ed elettroniche (WEEE)" o le ordinanze locali Aviso de eliminación: elimine el instrumento de conformidad con la Directiva 2012/19/UE sobre residuos de aparatos eléctricos y electrónicos (RAEE) o las ordenanzas locales Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 16 TÜV SÜD Marque de certification – Type testé ; production contrôlée TÜV SÜD-Prüfzeichen – Typ geprüft; Produktion überwacht Marchio di certificazione TÜV SÜD: tipo testato, produzione monitorata Marca de certificación TÜV SÜD: tipo probado, producción controlada Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 17 SJ/T11364-2006 de la República Popular China para la industria electrónica. Las sustancias tóxicas o peligrosas no se filtrarán ni mutarán en condiciones de funcionamiento normales durante 40 años. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 18 Gran Bretagna. El marcado UKCA (UK Conformity Assessed) es una marca de certificación que indica la conformidad con los requisitos aplicables para los productos vendidos en Gran Bretaña. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 19 Aduanera Euroasiática. Significa que los productos con la marca EAC MED cumplen todos los requisitos de los reglamentos técnicos correspondientes y han superado todos los procedimientos de evaluación de conformidad. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 20 Requisiti di etichettatura ACMA El producto cumple con los requisitos de comunicaciones de Australia. EESS: marcado RCM (Regulatory Compliance Mark) de cumplimiento de la normativa. Requisitos de etiquetado de ACMA Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 21 SAR de equipos eléctricos y electrónicos. Los requisitos de EMC se aplican a los productos Agilent. Temperature limit Temperatuur limiet Limite de temperature Temperaturgrenze Limite di temperature Límite de temperatura Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 22 Symbols| 21 Humidity limitation Vochtigheidsbeperking Limitation d'humidité Feuchtigkeitsbegrenzung Limitazione dell'umidità Limitación de humedad Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 23: Conformance To Standards

    General Requirements CAN/CSA C22.2 No. 61010-2-010 Safety Requirements for Electrical Equipment For Measurement, Control, and Laboratory Use – Part 2-010: Particular requirements for laboratory equipment for the heating of materials Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 24: Emc Information And Technical Description

    IP 20. Protected against solid foreign objects of 12.5 mm diameter and greater. No protection against water. Disposal Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 25: Introduction

    Chapter 1 Introduction This chapter introduces the Synergy LX and provides contact information for technical assistance. Product Description Package Contents Optional Accessories Technical Support...

  • Page 26: Product Description

    SLXFATS-SN contain both light sources. Only the halogen bulb is user-replaceable. Use of labware other than described here can result in positioning errors during program execution. See Appendix A, Specifications, for performance and technical specifications. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 27: Package Contents

    Package Contents | 3 Package Contents Item Part # Synergy LX User Manual on USB flash drive 1501000N Power supply 01281 Power cord Varies by country USB cable 75108 Blank USB flash drive (Touchscreen models) 1560531 7/64" hex wrench (for opening bulb access 48169 panel) 3/32"...

  • Page 28: Optional Accessories

    7260551 Fluorescence Test Plate 1400501 Luminescence Test Plate (Harta Luminometer Reference Microplate) (includes microplate carrier adapter PN 8032028 for 8030015 Synergy LX) Synergy LX Product Qualification (IQ-OQ-PQ) package 1500534N Take3 Micro-Volume Plate TAKE3 Take3 Trio Micro-Volume Plate TAKE3TRIO BioCell Adapter Plate...
  • Page 29 Manufacturer part numbers are subject to change. Item Part # Absorbance Liquid Tests BioTek Wetting Agent Solution 7773002 BioTek QC Check Solution #1 (25 mL) 7120779 BioTek QC Check Solution #1 (125 mL) 7120782 Phosphate-Buffered Saline (PBS) tablets, pH 7.2-7.6 Sigma #P4417 98233 or β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, reduced...

  • Page 30: Technical Support

    Save As to save a text file of the system test report, which can be emailed to Technical Support. If you need to return an instrument to BioTek for service or repair, please contact Technical Support for instructions. Repackage the instrument according to the instructions at the end Chapter 2, Installation.

  • Page 31: Installation

    Chapter 2 Installation This chapter includes instructions for unpacking and setting up the Synergy LX. Instructions are also included for preparing the reader for shipment. Important Information 1. Unpack the Box and See What’s Inside 2. Remove the Shipping Screw and Insert the Light-Blocking Plug 3.

  • Page 32: Important Information

    This chapter contains installation and setup tasks for the Synergy LX and accessories. Perform the tasks in the order presented. Save all packaging materials. Be sure to use packaging materials supplied by the manufacturer when shipping the reader.

  • Page 33: Unpack The Box And See What's Inside

    Remove the plastic bag and remove the desiccant pack. 3. Save the box, bag, foam top tray and corner cubes, and accessories tray in case you need to send the reader to BioTek for service. Synergy LX User Manual BioTek Instruments, Inc.

  • Page 34: Remove The Shipping Screw And Insert The Light-Blocking Plug

    1. From the back of the reader: Remove the shipping screw. At the front of the reader: 2. Open the upper access door. Insert the shipping screw into its storage location. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 35 2. Remove the Shipping Screw and Insert the Light-Blocking Plug | 11 3. Below this storage location, remove the rubber light-blocking plug. At the back of the reader: Insert the plug into the hole left open during step 1. Twist to seat. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 36: Gen5 Control Only) Prepare The Host Computer

    Gen5 software versions 3.11 and higher require Windows 10. You must have administrator privileges to install Gen5. Log in to Windows as “Administrator” or consult your IT department for assistance. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 37: Gen5 Control Only) Connect The Host Computer And Reader

    2. Plug the rounded end of the external power supply's cord into the power inlet, and tighten the collar by turning it clockwise. 3. Connect the power cord to the power supply, and plug it into an appropriate power receptacle. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 38 | Chapter 2: Installation Power cord (left) and power supply (right) are supplied Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 39: Turn On The Reader And Run The Power-Up System Test

    OK. The reader name and serial number appear. Click on the reader name and then click Test Communications. The message, “The reader is communicating!” should appear. If the communication attempt is not successful, try the following: Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 40: Operational/Performance Qualification

    BioTek following service or repair, or if regulatory requirements dictate that Operational/Performance Qualification is necessary, turn to Chapter 6, Instrument Qualification. A Product Qualification & Maintenance (IQ/OQ/PQ) package for the Synergy LX is available for purchase (PN 1500534N). Contact your local BioTek dealer for more information. Synergy LX User Manual BioTek Instruments, Inc.

  • Page 41: Repackaging And Shipping Instructions

    Repackaging and Shipping Instructions | 17 Repackaging and Shipping Instructions Important! Please read all of the information provided below before preparing the Synergy LX for shipment. Contact Technical Support before returning equipment for service. Decontamination prior to shipment is required by the U.S. Department of Transportation regulations.
  • Page 42 4. If the microplate carrier is inside the reader, press the carrier button to extend it. 5. Power off the reader. 6. Without moving the reader, disconnect the power and communication cables. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 43 9. Use the supplied 3/32” hex wrench to firmly seat the shipping screw. Test this by tipping up the back of the reader. If the carrier does not move, the reader is ready for repackaging. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 44 4. Place the accessory tray in the box, and then place the accessories into the tray as shown in the figure. 5. Close the top of the box, and secure it with shipping tape. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 45 Repackaging and Shipping Instructions | 21 Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 46 | Chapter 2: Installation Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 47: Getting Started

    Chapter 3 Getting Started This chapter provides an introduction to the Synergy LX. It also contains recommendations for optimum performance. External Components Filter Cubes Operate the Reader Using the Touchscreen Run a Take3 Session Using the Touchscreen Getting Started with Gen5 Software...

  • Page 48: External Components

    5. front compartment access door; shown open with touchscreen behind it 1. USB port for printer 2. power inlet 3. USB port for host computer 4. shipping screw 5. fan filter access Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 49 2. microplate carrier access door 3. power on/off 4. carrier in/out, status LED 5. front compartment access door 1. power inlet 2. USB port for host computer 3. shipping screw 4. fan filter access Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 50: Filter Cubes

    Do not open the door to access the filter cube during instrument operation! Doing so may result in invalid data. If necessary, disconnect the USB cable to regain access to touchscreen control. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 51 All F models of the Synergy LX ship with a LUM filter cube. Other filter cubes can be ordered from BioTek. Four preconfigured filter cubes, as well as an empty cube, are available from BioTek. Filter Cube/PN EX Wavelength/Bandpass EM Wavelength/Bandpass...
  • Page 52 1. Open the access door on the reader’s front panel. 2. Slide the filter cube into the filter cube port, making sure the label on the filter cube handle is right-side up. See the photo below. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 53 Do not use a sharp instrument when removing or replacing a filter or C-clip filter retainer. Do not touch the filters or mirror with bare fingers. Handle the mirror by its edges. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 54 | Chapter 3: Getting Started Tools 7/64 hex key (included with the Synergy LX) Lens paper Linen or cloth gloves (for handling the mirror) Procedure 1. Remove the screw and EM filter holder. 2. Using the following diagram, install the apertures, filters, C-clips, and mirror. Replace the EM filter holder when finished.
  • Page 55 Filter Cubes | 31 Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 56 Ensure that the paper roll is installed so that the shiny side of the paper faces up. The printer will not print on the matte side of the paper. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 57 To cleanly tear the paper from the printer, pull upward from left to right. To advance the paper, press Feed on the front of the printer. To turn off the printer, press and hold the ON/OFF button for at least three seconds. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 58: Operate The Reader Using The Touchscreen

    The left side of the Main Menu lists the five protocols most recently created and saved or edited on the reader. In the middle are the protocols created for each available detection method and for use with the Take3 plate (if configured; see page 45). Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 59 Run a system test, define date/time settings, enable output to a printer and/or USB flash drive, define filter cubes (models with fluorescence/luminescence capability), and configure a Take3 plate (models with absorbance capability). Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 60 | Chapter 3: Getting Started Configure Your Synergy LX After you install the reader, and before you use it to create and run protocols, perform the tasks in this section to define important instrument settings. Confirm or Set the Time and Date 1.
  • Page 61 1. From the Main Menu, tap Quick. 2. Select the tab for the detection method you wish to use, then select the plate type and define the appropriate settings for your assay. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 62 Save. Note that the protocol name is limited to 18 characters. 3. Define the protocol parameters, and then tap Save. The next three sections describe the available parameters for each of the three possible detection methods. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 63 Use the options here to include a delay (up to 15 minutes) or shake before the read. See page 48 for information on the available shake types. When finished, tap Save. The protocol name is added to the Main Menu. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 64 It is also stored with the results and will be included in the output to printer and/or USB flash drive. Synergy LX User Manual BioTek Instruments, Inc.
  • Page 65 Plate: Tap the field to select a plate type. Options include standard 96- and 384-well plates and Take3 if the plate has been configured on the reader (see page 46). Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 66 Activity Before Read: Use the options here to include a delay (up to 15 minutes) or shake before the read. See page 48 for information on the available shake types. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 67 2. To read a partial plate, tap Full Plate and then select the column(s) to read. Tap Save. Important: If running a Fluorescence or Luminescence protocol, ensure that the correct Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 68 USB flash drive. Edit, Delete, or Copy a Protocol The Synergy LX ships with several preprogrammed protocols. Depending on the reader’s model, these protocols include Absorbance (BCA and Bradford), Fluorescence  (Red, Blue, and Green), Luminescence (Lum (unfiltered)), and the seven absorbance Take3 protocols.

  • Page 69: Run A Take3 Session Using The Touchscreen

    Take3 plate has been defined and calibrated. These protocols are not editable and are read at the wavelengths 260, 280, and 320 nm. For nucleic acid protocols, a secondary wavelength of 230 nm can be selected. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 70 2. Enter the Take3 plate’s serial number, and place the Take3 plate on the carrier. Tap Required and then Start to perform the alignment process. Note that this process can take several minutes. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 71 USB flash drive, if enabled (see page 44). The results are also available for review and output by selecting Results from the Main Menu. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 72: Getting Started With Gen5 Software

    2. In the Gen5 Task Manager, select Protocol > Create New. 3. Open the Procedure dialog. If prompted to select a reader, select Synergy LX and click 4. Select a Plate Type to match your assay plate.
  • Page 73 Getting Started with Gen5 Software | 49 The Optics Type is already set for the Synergy LX (Monochromators). Choose a Detection Method: Absorbance, Fluorescence intensity, or Luminescence. Choose a Read Type: Endpoint/Kinetic, Spectral Scanning, or Area Scanning. Click OK. The Read Step dialog contains parameters specific to the Detection Method. Click the Help button for guidance.

  • Page 74: Recommendations For Optimum Performance

    4. When the read is complete, measurement values appear in Gen5. Select the desired data set from the Data list. 5. Select File > Save and give the file an identifying name. Features Supported by Synergy LX That Require Gen5 Reader qualification Performing fluorescence reads in more than one color...
  • Page 75 When using a 384-well microplate, you can use the Gen5 Auto Map feature to ensure you are using an accurate plate map for your reads. See the Gen5 Help for more information. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 76: Periodic Maintenance

    Chapter 4 Periodic Maintenance This chapter provides instructions for cleaning and decontaminating the Synergy LX. Maintenance Overview Warnings and Precautions Clean Exposed Surfaces Clean the Touchscreen Inspect/Clean Filter Cubes Clean the Air Filter Replace the Halogen Bulb Assembly Decontamination...

  • Page 77: Maintenance Overview

    Deionized or distilled water Clean, lint-free cotton cloths Sodium hypochlorite (NaClO, or bleach) (decontamination only) Safety glasses Surgical mask Protective gloves Lab coat Biohazard trash bags 125-mL beakers Cotton swabs or paper towels Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 78: Warnings And Precautions

    Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible. Wear protective gloves when handling contaminated instruments. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 79: Clean Exposed Surfaces

    Periodic Maintenance Clean Exposed Surfaces This procedure is for the housing of the Synergy LX instrument. See Clean the Touchscreen on page 55 for the cleaning procedure for the touchscreen. A regular cleaning regimen is recommended to keep the instrument free from dust and particulates that can cause erroneous readings.

  • Page 80: Inspect/Clean Filter Cubes

    1. Open the top access door on the instrument’s front panel, and slide the filter cube out of its port. 2. Inspect the glass filters for speckled surfaces or a “halo” effect. This may indicate deterioration due to moisture exposure over a long period of time. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 81: Clean The Air Filter

    Clean the Air Filter 1. Using either a flat-blade or #2 Phillips screwdriver, remove the screw that secures the air filter cover on the rear of the reader. Remove the cover and filter. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 82 2. Rinse the air filter in water, then allow it to dry completely. 3. Replace the air filter and air filter cover on the reader, and secure them in place with the screw. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 83: Replace The Halogen Bulb Assembly

    5. Screw in the thumbscrew, then finger-tighten to secure the lamp. Plug in the lamp’s power plug. 6. Replace the side door and secure it with the two screws. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 84: Decontamination

    Decontamination | 60 Decontamination The Synergy LX requires decontamination prior to shipping, storage, and disposal. Decontamination is required by the U.S. Department of Transportation regulations. Persons performing the decontamination process must be familiar with the basic setup and operation of the instrument.
  • Page 85 8. Use a clean, dry lint-free cloth to dry all wet surfaces. 9. Discard the used gloves and cloths using a biohazard trash bag and an approved biohazard container. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 86: Instrument Qualification Process

    Instrument Qualification Process This chapter describes the tests that BioTek Instruments, Inc., has developed for complete qualification of all models of the Synergy LX. This chapter introduces the various test methods, describes the materials and protocol parameters used to execute the tests, explains how to analyze test results, and provides troubleshooting tips in the event of a failure.

  • Page 87: System Test

    If any test results do not meet the internally coded Failure Mode Effects Analysis (FMEA) criteria established by BioTek, the reader beeps repeatedly, and, if equipped, an error message appears on the touchscreen. If this occurs, either press the carrier eject button, or, if equipped, tap on the touchscreen to stop the beeping.

  • Page 88: Absorbance Testing

    Absorbance Testing Applies only to models with absorbance capability BioTek developed a series of tests for the absorbance system using a combination of solid state Absorbance Test Plates and liquid plates. An advantage of running liquid tests is the liquid in the wells has a meniscus, whereas the test plate’s neutral density glass filters do not.
  • Page 89 If a higher-OD well reports "#N/A" for Min/Max Limit and Result, the measured OD is beyond the specified range for Accuracy or Repeatability used with this test, and therefore no pass/fail determination is made. It does not indicate a test failure. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 90 Check the calibration due date on the test plate’s label. If the test plate is overdue for recalibration, contact BioTek to schedule service. Check the microplate carrier to ensure it is clear of debris.
  • Page 91 Absorbance Liquid Test 3 is an optional test offered for those sites that must have proof of linearity at 340 nm. (Alternatively, the BioTek 340 nm Absorbance Test Plate may be used; see page 64.) This test is optional since the Synergy LX has good “front end”...
  • Page 92 Read wells Full plate Wavelength 2 (450 nm, 630 nm) Read Speed Normal Delay after plate movement 100 msec Plate Out,In step between loops Text "Please rotate the plate 180 degrees" Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 93 2. For each well in columns 1 and 2, calculate the Allowed Deviation using the Repeatability specification for a 96-well plate (Mean OD x 0.01 + 0.010). For each well, its Standard Deviation should be less than its Allowed Deviation. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 94 0.9900 is considered adequate. 2. Calculate the results for Repeatability: Calculate the Mean and Standard Deviation for the five readings taken at each concentration. Only one row of data needs to be analyzed. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 95 2. For each Mean calculated in step 1, calculate the Allowed Deviation using the Repeatability specification for a 96-well plate (Mean OD x 0.015 + 0.005). For each well, its Standard Deviation should be less than its Allowed Deviation. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 96 Liquid Test 1 can fail due to the meniscus effect, which can cause readings to decrease over time. If you suspect this may be the case, include a shake step between the read steps in the protocol. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 97: Fluorescence Testing

    Instrument Qualification Process Fluorescence Testing For models with fluorescence capability, BioTek provides two options for testing the fluorescence system. One uses a solid state Fluorescence Test Plate (package PN 1400501*). The other uses liquid plates, the materials for which are available for purchase...
  • Page 98 The information in the following tables represents the recommended reading parameters. It is possible that your tests will require modifications to some of these parameters, such as the Plate Type (see Troubleshooting Tips on page 79). Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 99 Gain: Auto, Use first filter set gain from FIRST Read Step Read Speed: Normal Delay after plate movement: 350 msec Measurements per data point: 10 Dynamic Range: Standard Read Height: 8.75 mm Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 100 Plate Type: Costar 96-well black opaque (#3915) Detection Method: Fluorescence Three Read Steps Read Step 1: Kinetic Kinetic loop: Run Time: 0:00:45 Interval: 0:00:03 Reads: 16 Step Label: Sensitivity Read Read Well: Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 101 Filter Set: Blue Gain: Auto, Scale to High Wells, C1, 80000 Read Speed: Normal Delay after plate movement: 350 msec Measurements per data point: 10 Dynamic Range: Standard Read Height: 7.25 mm Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 102 Methylumbelliferone: Using the known concentration value of MUB and the calculated SNR: 17.6/SNR To pass, the Detection Limit Filter Cube must be: Blue: 360/40, 460/40, 400 nm <= 0.16 ng/mL (0.91 nM) dichroic mirror Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 103 Technical Support. Are the solutions fresh? Discard the plate and any opened, unused test solutions after seven days. Are the excitation/emission filters clean? Are you using the proper filter cube? Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 104 The PMTs or optical path(s) may be deteriorating, or the optics or other hardware may be misaligned. Contact Technical Support. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 105: Luminescence Test

    For models with luminescence capability, BioTek uses the Harta Luminometer Reference Microplate to test the luminescence system. The test plate is LED-based and NIST- traceable. Contact BioTek to purchase a plate (BTI #8030015; includes microplate carrier adapters) or visit www.HartaInstruments.com to learn more.
  • Page 106 8030015 Harta with 8032028 adapter Delay Step: 3 minutes Read Step 1: Luminescence Detection Method: Endpoint Read Type: Step Label: Reference well A2 Read Wells: Filter Set: Gain: Integration Time: 0:10.00 MM:SS.ss Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 107 Read Type: Endpoint Read Wells: A7–A8 Step Label: Battery Check Filter Set: Gain: Integration Time: 0:01.00 MM:SS.ss Delay After Plate Movement: 350 msec Dynamic Range: Extended Read Height: 8.5 mm Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 108 Verify that the filter cube settings in Gen5 match the filter cube installed in the reader. If the test continues to fail, the optics block may need to be cleaned. Contact Technical Support for instructions. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 109: Instrument Qualification Procedures

    Instrument Qualification Procedures This chapter contains the step-by-step procedures for verifying that the Synergy LX and its various subsystems are performing to specification. Chapter 5, Instrument Qualification Process, starting on page 62, introduces the various test methods, describes the materials and relevant protocol parameters used to execute the tests, explains how to analyze test results, and provides troubleshooting tips in the event of a failure.

  • Page 110: Overview

    A Product Qualification Package (PN 1500534N) for the Synergy LX is available for purchase. The package contains complete procedures, Gen5 protocols, checklists, and logbooks for performing Installation Qualification, Operational Qualification, Performance Qualification, and Maintenance.
  • Page 111 This frequency may be adjusted depending on the trends observed over time. The successful completion of the PQ procedure confirms that the equipment is performing consistently under normal operating conditions. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 112: Recommended Qualification Schedule

    | Chapter 6: Instrument Qualification Procedures Recommended Qualification Schedule This table defines BioTek-recommended intervals for qualifying a Synergy LX used two to five days a week. The actual frequency, however, may be adjusted depending on your usage of the instrument and its various models. The schedule assumes that the instrument is properly maintained as outlined in the Periodic Maintenance chapter.

  • Page 113: System Test

    System Test System Test, starting on page 63, describes this test and explains where to find information on error conditions, as well as sample test reports for Synergy LX. Test Procedure Using the Touchscreen 1. From the Main Menu, tap Instrument, then select the Configuration tab.

  • Page 114: Absorbance Plate Test

    | Chapter 6: Instrument Qualification Procedures Absorbance Plate Test BioTek Absorbance Test Plate, starting on page 64, describes the test methods and provides troubleshooting tips in the event of test failure. Requirements To perform this test, you will need: Absorbance Test Plate, PN 7260522 (Optional) 340 nm Absorbance Test Plate, PN 7260551...
  • Page 115 8. When the test is complete, the results report appears. Scroll through the report; every result should show “PASS”. Troubleshooting tips are provided on page 65. Test descriptions are provided on page 64. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 116: Absorbance Liquid Tests

    5-mL Class A volumetric pipette 100-mL volumetric flask 1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask. 2. Add 95 mL of DI water; cap and shake well. The solution should measure approximately 2.000 OD when using 200 µL in a flat-bottom microwell.
  • Page 117 1. Weigh out 0.092 gram of FD&C No. 5 yellow dye powder into a weigh boat. 2. Rinse the contents into a 1-liter volumetric flask. 3. Add 0.5 mL of Tween 20 or 5 mL of BioTek’s wetting agent. 4. Make up to 1 liter with DI water; cap and shake well.
  • Page 118 Solution A or B (see the instructions for Liquid Test 1) A 0.05% solution of deionized water and Tween 20 The Gen5 protocol Synergy LX Abs Test 2.prt, described on page 68 Test Procedure 1. Create a percentage dilution series, beginning with 100% of the original concentrated stock solution (A or B) in the first tube, 90% of the original solution in the second tube, 80% in the third tube, all the way to 10% in the tenth tube.
  • Page 119 Alternatively, wait 20 minutes after pipetting the test solution before reading the plate. 4. Create a Gen5 experiment based on the Synergy LX Abs Test 2 protocol and read the plate. When prompted, rotate the plate 180 degrees. 5. When finished: Save the experiment.
  • Page 120 Absorbance Liquid Test 3 is provided for sites requiring proof of linearity at 340 nm. This test is optional because the Synergy LX has good "front end" linearity throughout its wavelength range. As an alternative, the 340 nm Absorbance Test Plate (PN 7260551) may be used for this test.
  • Page 121 Alternatively, wait 20 minutes after pipetting the test solution before reading the plate. 4. Create a Gen5 experiment based on the Synergy LX Abs Test 3 protocol and read the plate. Save the experiment. Refer to the instructions on page 69 to perform calculations and determine pass/fail.

  • Page 122: Fluorescence Plate Tests

    Instrument Qualification Procedures Fluorescence Plate Tests BioTek Fluorescence Test Plate on page 73 introduces the test plate and references its user guide for the test methods. Use of the test plate is offered as an alternative to conducting the fluorescence liquid tests described in the next section.
  • Page 123 Scientific 1 L Sodium Borate Mfr. #159532, or equivalent), Phosphate-Buffered Saline (PBS), pH 7.2–7.6 (e.g., Sigma tablets, Mfr. #P4417, or equivalent) and pH meter or pH indicator strips with pH range 4 to 10 Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 124 Gen5 Test Solutions If using BioTek’s sodium fluorescein powder (PN 98155), be sure to hold the vial upright and open it carefully; the material may be concentrated at the top. If a centrifuge is available, spin down the tube before opening.
  • Page 125 Pipette 200 μL of the 1 nM SF solution into well D7. Pipette 200 μL of the buffer solution into wells C9, D9, and E9. Using a multichannel pipette with just four tips installed: Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 126 Alternate/Supplemental Tests Using Methylumbelliferone (MUB) As an alternative to using Sodium Fluorescein, Methylumbelliferone (“MUB”) can be used to test the fluorescence system. Required Materials Manufacturer part numbers are subject to change over time. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 127 Fluorescence Liquid Tests | 103 BioTek offers a liquid test kit (PN 7160012) containing the microplates and solutions used in the MUB fluorescence liquid tests. Methylumbelliferone (“MUB”) (10-mg vial, BioTek PN 98156) Carbonate-Bicarbonate buffer (“CBB”) capsules (BioTek PN 98158) 100% methanol (BioTek PN 98161) A new, clean 96-well solid black plate microplate, such as Corning Costar #3915 or equivalent.
  • Page 128 3. Refer to the instructions starting on page 102 to calculate results and determine pass/fail. Troubleshooting tips are provided on page 78 for results analysis. Test descriptions are provided on page 74. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 129 Aspirate 150 µL from column 4 and dispense it into column 5. Mix the wells using the pipette. Do not discard the tips. Aspirate 150 µL from column 5. Discard the tips. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 130: Luminescence Test

    2. Check the plate’s battery by pressing the test button on the back of the plate and ensuring that the test light turns on. If the light does not turn on, replace the battery. Synergy LX User Manual BioTek Instruments, Inc.
  • Page 131 5. When the experiment is complete, calculate and evaluate results as described under Results Analysis on page 81. 6. When finished, turn off the Harta reference plate to preserve battery life. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 132: Specifications

    Appendix A Specifications This appendix contains BioTek's published specifications for the Synergy LX. General Specifications Absorbance Specifications Fluorescence Specifications Luminescence Specifications...

  • Page 133: General Specifications

    General Specifications Microplates The Synergy LX accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry and the Take3 Micro-Volume Plate. If using Gen5, the Take3 Trio Micro-Volume plate, the BioCell, and 60-, 72-, and 96-well Terasaki plates are also supported.

  • Page 134: Absorbance Specifications

    = 100 ms 1.500 to 2.000 OD: ±5.0% ±0.010 OD, delay after plate movement = 100 ms 96- and 384-well plate, sweep read 0.000 to 1.000 OD: ±1.0% ±0.010 OD speed Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 135 = 100 ms 96- and 384-well plate, sweep read 0.000 to 1.000 OD: ±2.0% ±0.010 OD speed: Assay Validation Using a Take3 Plate 260 nm dsDNA detection limit: < 5 ng/μL Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 136: Fluorescence Specifications

    PMT Gain: Blank Wells: Wavelength Range: 200–700 nm 96-well plate with red-shifted PM Detection Limit for ATP: ≤ 500 amol/well Integration Time: 10 seconds PMT Gain: Blank Wells: Wavelength Range: 200–850 nm Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 137: Sample Reports

    Appendix B Sample Reports This appendix contains sample System Test and Absorbance Plate Test reports for the Synergy LX.

  • Page 138: Sample System Report

    | Appendix B: Sample Reports Sample System Report Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 139 Sample System Report | 115 Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 140: Sample Absorbance Test Plate Report

    | Appendix B: Sample Reports Sample Absorbance Test Plate Report Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 141: Sample Assay Results

    Sample Assay Results | 117 Sample Assay Results Sample (Report) CSV File Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 142 | Appendix B: Sample Reports Sample (Gen5 input) TXT File Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 143: Error Conditions

    Appendix C Error Conditions This appendix describes error conditions and provides troubleshooting tips. Overview Error Codes...

  • Page 144: Overview

    Some problems can be solved easily, whereas others can be solved only by trained BioTek service personnel. Error codes beginning with “A” (e.g., A100) indicate conditions that require immediate attention.
  • Page 145 The monochromator hardware module is not available. A500 EEProm memory read/write error AB00 Multiprocessor error AC00 External RAM error ADXX Stack error AEXX Invalid motor ramp profile 7FXX Dual processor error Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 146 Absorbance measurement channel producing inconsistent reoccurs, contact Technical 0412 or noisy data. Support. Voltage reference measurement data converter never 040F indicated “ready.” Voltage reference measurement channel producing 041F inconsistent or noisy data. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 147 Lamp current is noisy or inconsistent. Mono flash absorbance minimum power test 0604 Flash voltage is outside limits. Mono flash absorbance minimum power test 0614 Flash voltage is noisy or inconsistent. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 148 Turning the 0800 Processor EEPROM configuration memory checksum instrument off and on may 0801 mismatch resolve the problem. If the 0802 error reoccurs, contact Technical Support. 20XX errors Command definition error Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 149 • Instrument serial number • Gen5 version (available under Help > About Gen5) • An overview of the protocol definition • The plate type used and whether it is standard or custom defined Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 150 Autosensitivity—invalid target value 210A Invalid event number 210B Event type should be a read 210C Shake carrier—invalid shake frequency 210D Unknown diagnostic command 210E Invalid autocalibration selection 2110 Wavelength calibration—invalid wavelength Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 151 23XX errors Item not found error This indicates that a command is requesting an item that is not present in the instrument configuration memory (for example, a specific wavelength filter). Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 152 These errors are generated when a protocol entry is incorrect (setpoint, volume, etc.). These errors should not occur because Gen5 should trap them during protocol creation. If a 2DXX error does occur, contact Technical Support. 2D01 Invalid readset count. Invalid number of readsets selected. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 153 Invalid shake type. Specified shake type selected. • Run system self-test or turn instrument off and on. 2D99 Unit in debug mode self-test may not have been run. • If error is repeatable, contact Technical Support. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 154 Absorbance measurement channel failed offset test. • Ensure the filter cube access door is closed. 39XX errors Absorbance dark range outside of limits Fails if dark offset range is < 10 counts or > 16238 counts. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 155 • The filter cube access door Absorbance measurement channel saturation y= test is closed. 3E1Y wavelength #. Contact Technical Support. 3EX2 Ratio between channels out of range. 3FXX errors Absorbance signal out of range Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 156 8000 counts, a signal error is flagged. Note: This error may not occur during a system test but can occur when the first read is attempted. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 157 8000 counts. Technical Support. Measurement/Reference ratio error If the unfiltered 3F2Y reference signal is greater than 18% of the measurement signal, a signal ratio error is flagged. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 158 Verify that the physical filter configuration matches Gen5 filter table. Well location/error code for 48-well plate Note: Follow the same patterns for 96-well and 384-well plates. 47XX errors PMT Fluorescence noise test error Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 159 Technical Support. 4BXX errors PMT operations or test error Ensure that door is fully closed and instrument cover 4B0X PMT Reference channel error is secured. Too much light in chamber. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 160 Open hole in emission 4F00 Fluorescence reference signal out of range and/or excitation filter cubes. Ensure that Gen5 Fluorescence/Luminescence wavelengths table matches the filters physically installed in the filter cubes. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 161 5001 Carrier Y failed to home An object may be 5201 obstructing the path. Defective or broken optical sensor. If repeatable, contact Technical Support. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 162 Run self-test or power cycle 5501 Carrier Y-Axis not homed successfully instrument If repeatable or reoccurs, contact Technical 5502 Vertical Z-Axis not homed successfully Support. 5504 Monochromator not homed successfully Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 163 If retry is unsuccessful, run a system test and try once more using the correct plate height. 5801 Carrier Y alternate sensor error If repeatable or reoccurs, contact Technical Support. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 164 Probe Z windup error If repeatable or reoccurs, contact Technical Support. 5E00 No Peak found error If repeatable or reoccurs, 6XXX 6XXX errors Calibration data missing or corrupted contact Technical Support. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 165 Filter wavelengths are 2115 total bandwidth, do not overlap. Saturation of overlapping PMT may result. Dichroic wavelength Ensure that configuration table matches 2116 must be between filter physical configuration wavelengths Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 166 Note: 50XX errors indicate problems with a device connected to a USB port, either flash drive or printer. If the device connected is a flash drive, try a different drive. If a printer, ensure that the printer is powered on and in the “ready” state. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 167 Take3 plate that has not been calibrated, this supported error will result. To use a new Take3 plate, the serial number must be entered and the plate calibrated before selecting it for a protocol. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 168 The reader will lower gain values for a 2nd try, 7FF4 determining AutoScale but if wells are still out of range, this error will result AutoScale failed with 7FF5 over range well Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 169 If repeatable or reoccurs, contact Technical response Support. Error waiting to send 7FFC command Checksum error parsing 7FFD command response 81XX Communication error Basecode versions do A200 not match AC00 External RAM error Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 170: Safety Information

    Appendix D Safety Information Veiligheidsmededelingen Avis de sécurité Sicherheitshinweise Avvisi di sicurezza Avisos de seguridad This appendix contains safety information for the Synergy LX, translated into Dutch, French, German, Italian, and Spanish. Safety Notices...

  • Page 171: Safety Notices

    Produktdokumentationen. Prestare particolare attenzione agli avvisi di sicurezza presenti in tutta la documentazione del prodotto. Preste especial atención a los siguientes avisos de seguridad en toda la documentación del producto. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 172 No siga adelante sin antes comprender y cumplir plenamente los requisitos indicados en el aviso de ADVERTENCIA. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 173 No siga adelante sin antes comprender y cumplir plenamente los requisitos indicados en el aviso de PRECAUCIÓN. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 174 Tensión interna. Siempre apague el interruptor y desconecte la fuente de alimentación antes de limpiar la superficie exterior del instrumento. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 175 El uso de un receptáculo incompatible puede producir descargas eléctricas y riesgo de incendio. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 176 Conecte siempre el cable de alimentación directamente a un receptáculo adecuado con una toma de tierra funcional. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 177 Fuente de alimentación. Use únicamente la fuente de alimentación incluida con el instrumento y úsela en el rango de tensiones de línea indicado en ella. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 178 Potenzielle Biogefahren. Manche Assays oder Proben stellen eine Biogefahr dar. Es sollten angemessene Sicherheitsvorkehrungen entsprechend der Packungsbeilage des Assays ergriffen werden. Tragen Sie immer eine Schutzbrille und eine geeignete Schutzausrüstung, wie chemikalienbeständige Gummihandschuhe und Schürze. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 179 Use siempre gafas de seguridad y equipos protectores adecuados, como guantes de caucho resistentes a productos químicos y un delantal. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 180 Limpie el derrame inmediatamente. No utilice el instrumento si los componentes internos han estado expuestos a fluidos. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 181 él. No permita que entre agua ni otra solución de limpieza en el interior del instrumento. Si esto sucediera, póngase en contacto con el servicio de soporte técnico. No remoje la pantalla táctil. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 182 Especificaciones de este documento. Si las temperaturas fluctúan por encima o por debajo de este rango, el rendimiento puede verse afectado negativamente. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 183 20 minutos. Un contacto demasiado prolongado puede dañar las superficies del instrumento. Asegúrese de aclarar y secar concienzudamente todas las superficies. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 184 Lubricantes. No aplique lubricantes en las piezas móviles. El lubricante en los componentes del compartimento del portador atraerá polvo y otras partículas que pueden hacer que el instrumento muestre un error. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 185 Superficie caliente. El conjunto de piezas de la lámpara está caliente cuando el instrumento está encendido. Apague el lector y deje que la bombilla se enfríe durante al menos 15 minutos antes de proceder a cambiarla. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 186 Equipo de envío. Antes de utilizar el instrumento es necesario retirar todo el equipo de envío y, del mismo modo, habrá que volver a colocárselo cuando el instrumento se vaya a enviar. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 187 Inhalt des installierten Filterwürfels übereinstimmen. Gen5- Benutzer: Die Filterwürfeltabelle der Gen5-Software muss genau mit dem Inhalt des Filterwürfels übereinstimmen. Wenn Sie den Filterwürfel austauschen oder seinen Inhalt ändern, müssen Sie die Filterwürfeltabelle(n) aktualisieren. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 188 ¡No abra la puerta para acceder al cubo de filtro mientras el instrumento está en funcionamiento! Si lo hace, los datos podrían perder la validez. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 189 Pantalla táctil. Utilice la yema del dedo para tocar la pantalla táctil. No utilice agujas afiladas ni lápices sobre la pantalla táctil. Si lo hace, dañará la superficie. Puede utilizar una aguja diseñada para pantallas táctiles resistivas. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 190 Las partículas de suciedad y los agentes de limpieza se quedarán incrustados en los arañazos. Nunca pulverice soluciones directamente Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 191 Repuestos. Durante el mantenimiento, solo deben emplearse repuestos originales. El uso de repuestos y accesorios no autorizados puede producir la pérdida de la garantía y reducir el funcionamiento del instrumento o provocar daños en él. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 192 Manutenzione. Le procedure di manutenzione sui componenti interni devono essere eseguite esclusivamente da personale tecnico qualificato. Revisión. Solo puede realizar procedimientos de revisión de los componentes internos el personal técnico cualificado. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 193 à réaliser est considérée comme une bonne pratique de laboratoire. Ne pas exécuter les vérifications de contrôle de qualité peut produire des données de test erronées. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 194 Si no se realizan las comprobaciones de control de calidad, la prueba puede arrojar datos erróneos. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 195 Le caratteristiche di prestazione del software di riduzione dei dati non sono state stabilite con alcun test di diagnostica di laboratorio. Gli utenti devono valutare questo strumento e il software basato su PC congiuntamente ai Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 196 Uso no especificado. Si no se utiliza el equipo de conformidad con las directrices y salvaguardias especificadas en la documentación del producto para el usuario, se puede producir una situación de peligro. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...
  • Page 197 El uso de material de laboratorio diferente al descrito en este documento puede dar lugar a errores de posicionamiento durante la ejecución del programa. Synergy LX User Manual BioTek Instruments, Inc. 1501000N Revision A...

  • Page 198: In This Book

    In This Book This document contains installation, operation, maintenance, and qualification information for all models of the Synergy Document Revision History Part Number Revision Date Modifications 1501000N December 2021 First issue. Original Language – EN Manufactured by Agilent Technologies, Inc. 5301 Stevens Creek Blvd.

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