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Biotek ELx800 Operator's Manual

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Absorbance Microplate Reader
ELx800
™
Operator's Manual

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Summary of Contents for Biotek ELx800

  • Page 1 Absorbance Microplate Reader ELx800 ™ Operator’s Manual...
  • Page 3 When it becomes necessary to replace the bulb*, replacement kits are available from BioTek. PN 7330513 is required for standard models, and PN 7330516 is required for ELx800UV and ELx800NB models. The kit contains complete instructions for replacing and aligning the bulb.
  • Page 5 ELx800™ Absorbance Microplate Reader Operator’s Manual October 2006 © 2006 Part Number 7331000 Revision P ® BioTek Instruments, Inc.

  • Page 6: All Rights Reserved

    BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 7: Table Of Contents

    Directive 98/79/EC: In Vitro Diagnostics ..........xix Electromagnetic Interference and Susceptibility........... xx User Safety .................... xx Safety Symbols..................xxi Chapter 1: Introduction ..............1 Introducing the ELx800 Absorbance Microplate Reader ........2 Hardware Features ..................3 Software Features ..................3 Package Contents ..................4 Optional Accessories.................. 5 Specifications ...................
  • Page 8 Power-Up and System Test ..............18 ELx800 Main Menu ................18 Configuring Global Default Options ............19 Connecting a Printer to the ELx800 ............20 Setting Up the Serial Port for Communications With Other Devices ....22 Attaching the Cable................22 Installing Software on the Host PC ............
  • Page 9 Description ..................108 Requirements.................. 110 Defining the Absorbance Test Plate Parameters ........111 Running the Absorbance Plate Test............. 112 Test Failures ................... 113 Liquid Testing ..................114 Stock Solution Formulation ..............115 Liquid Test 1 ..................117 ELx800 Operator's Manual...
  • Page 10 Getting Started With Gen5 ..............150 Controlling the Reader With KC4™............152 Setting Up KC4................... 152 Problems ................... 153 Getting Started With KC4™ ..............153 Controlling the Reader With KCjunior™ ............ 154 Setting Up KCjunior ................154 Problems ................... 155 BioTek Instruments, Inc.
  • Page 11 Perform a 384-Well Plate Read in KCjunior™ ..........165 Appendix C: Report Format............169 Appendix D: Comparison of the ELx800 and the EL800....177 Appendix E: Instructions for Programming a New Assay ....179 Sample ANA Screen Enzyme Immunoassay Kit .......... 180 Intended Use ..................
  • Page 12 Figure 25: Procedure Dialog .............. 162 Figure 26: Read Step Dialog.............. 162 Figure 27: Plate Reading Dialog ............163 Figure 28: KC4 Main Menu, System|Readers........164 Figure 29: Reader Selection Dialog............. 164 Figure 30: New Data File Dialog ............165 BioTek Instruments, Inc.
  • Page 13 Figure 44: Assay Detail Report (Sheet 2 of 2) ......... 178 List of Tables Table 1: Recommended Qualification Schedule........103 Table 2: Typical Enzyme-Substrate Combinations and Stopping Solutions ... 115 Table 3: Test Tube Dilutions ..............120 Table 4: PBS 10X Concentrate Solution..........125 ELx800 Operator's Manual...

  • Page 14: Contact Information

    Service/TAC Phone: 800-242-4685 (toll free in the U.S.) 802-655-4740 (outside the U.S.) Fax: 802-655-3399 E-Mail: tac@biotek.com European Coordination Center BioTek® Instruments GmbH Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany Internet: www.biotek.de Phone: +49 (0) 7136 9680 Fax: +49 (0) 7136 968 111 E-Mail: info@biotek.de...

  • Page 15: Document Conventions

    DEFINE Text in COURIER font represents menu options as they appear on the instrument’s display. Note: Bold text is primarily used for emphasis. This icon calls attention to important information. ELx800 Operator's Manual...

  • Page 16: Revision History

    Changed plate type information to include 384-well and 72-/96-well Terasaki plate formats. Added reference to Quick Read on display. 09/98 Modified Appendix B - Computer Control. Updated Elx800 and EL800 model comparison table in Appendix F. 2/99 Incorporated Manual Updates.
  • Page 17 Added new Appendix B, Using 384-Well Geometry. Updated sample reports in Appendix C, Report Format (formerly Appendix D). Removed previous Appendix A, Decontamination. Removed previous Appendix C, Error Codes. Added new Appendix E, Instructions for Programming a New Assay. ELx800 Operator's Manual...
  • Page 18 Chapter 3: In System Startup, incorporated information from Manual Update Rev. N2 that not all software features discussed in the manual are available on ELx800 readers with custom programmed software. Chapter 4: - Changed title from “Performance Verification and IQ/OQ/PQ Procedures”...

  • Page 19: Intended Use Statement

    Failure to conduct Quality Control checks could result in erroneous test data. Repackaging and Shipping If you need to ship the instrument to BioTek for service or repair, contact BioTek for a Return Materials Authorization (RMA) number, and be sure to use the original packaging.

  • Page 20: Warnings

    Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into internal components creates a potential shock hazard. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid. BioTek Instruments, Inc.

  • Page 21: Precautions

    Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The ELx800™ should be serviced by authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components. Caution: Environmental Conditions.
  • Page 22 Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE).” Caution: Warranty. Failure to follow preventive maintenance . See protocols may void the warranty Chapter 5 Maintenance and Decontamination BioTek Instruments, Inc.

  • Page 23: Directive 89/336/Eec:electromagnetic Compatibility

    Traceability to the U.S. National Institute of Standards and Technology (NIST): Optical density measurements, and if equipped, incubator temperature readings, are traceable to NIST. Specific data for a particular serial number is available on request from BioTek Instruments. See page x for contact information. ELx800 Operator's Manual...

  • Page 24: Electromagnetic Interference And Susceptibility

    UL 61010A-1, 1st Edition “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements” International • IEC 1010 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements” BioTek Instruments, Inc.

  • Page 25: Safety Symbols

    Borne de terre de protection Schutzleiteranschluss Borne de tierra de protección Terra di protezione On (Supply) Marche (alimentation) Ein (Verbindung mit dem Netz) Conectado Chiuso Off (Supply) Arrêt (alimentation) Aus (Trennung vom Netz) Desconectado Aperto (sconnessione dalla rete di alimentazione) ELx800 Operator's Manual...
  • Page 26 Separate collection for electrical and electronic equipment Les équipements électriques et électroniques font l’objet d’une collecte sélective Getrennte Sammlung von Elektro- und Elektronikgeräten Recogida selectiva de aparatos eléctricos y electrónicos Raccolta separata delle apparecchiature elettriche ed elettroniche BioTek Instruments, Inc.

  • Page 27: Chapter 1: Introduction

    Chapter 1 Introduction This chapter introduces the ELx800™ Absorbance Microplate Reader and describes its hardware and software features. Also included is contact information if technical assistance is needed. Introducing the ELx800 Absorbance Microplate Reader ....2 Hardware Features..............3 Software Features..............3 Package Contents ..............

  • Page 28: Introducing The Elx800 Absorbance Microplate Reader

    The ELx800 is backed by a superior support staff. If the ELx800 ever fails to work perfectly, please call the Technical Assistance Center, or visit BioTek’s Web site. Refer to the end of this chapter for contact information.

  • Page 29: Hardware Features

    Hardware Features | Hardware Features The ELx800’s hardware features include: • Single optics channel • Wavelength range of 400-750 nm ELx800UV model provides extended wavelength range of 340 to 750 nm • A 5-position filter wheel • A 2-line x 24-character LCD display •...

  • Page 30: Package Contents

    Chapter 1: Introduction Package Contents The contents of the ELx800™ package include: • ELx800 Absorbance Microplate Reader • Power cord (part number varies according to country of use) • Power supply (PN 61062) • Filter wheel with 4 standard filters: 405 nm, 450 nm, 490 nm, 630 nm and one blank filter.

  • Page 31: Optional Accessories

    Gen5’s Reader Diagnostics Utility (PN 5320201) for the Absorbance Test Plate • Absorbance Liquid Test Solutions: BioTek Wetting Agent Solution (PN 7773002) BioTek QC Check Solution No. 1 (PN 7120779, 25 ml; or PN 7120782, 125 ml) • Epson LX-300 printer or equivalent (110V only) (PN 97145) •...

  • Page 32: Specifications

    8 kg (18.5 lb. Maximum) • Environment: Operating temperature 18° to 40°C • Humidity: 10% to 85% noncondensing • Power Supply: Input 100 to 240 V~ ± 10.0% @ 50 to 60 Hz Output +24 VDC, 2.1 A BioTek Instruments, Inc.

  • Page 33: Ultraviolet/Uv Model

    ± 2.0% ± 0.020 OD from 0.000 to 2.000 OD @ 405 nm Linearity: ± 2.0% from 0.000 to 2.000 OD @ 405 nm Repeatability (STD): ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm ELx800 Operator's Manual...

  • Page 34: Narrow Beam/Nb Model

    ± 2.0% ± 0.020 OD from 0.000 to 2.000 OD @ 405 nm Linearity: ± 2.0% from 0.000 to 2.000 OD @ 405 nm Repeatability (STD): ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm BioTek Instruments, Inc.
  • Page 35 ± 2.5% ± 0.020 OD from 0.000 to 2.000 OD @ 405 nm Linearity: ± 2.5% from 0.000 to 2.000 OD @ 405 nm Repeatability (STD): ± 2.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm ELx800 Operator's Manual...

  • Page 36: Product Support & Service

    A superior support staff backs all of BioTek’s products. If your instrument(s) or software ever fails to function perfectly, if you have questions about how to use or maintain it, or if you need to send an instrument to BioTek for service or repair, please contact our Technical Assistance Center (TAC).

  • Page 37: Chapter 2: Installation

    Chapter 2 Installation This chapter includes instructions for unpacking and setting up the ELx800™ and instructions for connecting printers and/or serial devices. Unpacking and Inspecting the ELx800™ ........12 Unpacking the Instrument and Its Accessories ......13 Setting Up the ELx800............. 17 Operating Environment ............

  • Page 38: Unpacking And Inspecting The Elx800

    . If the original packing materials have been damaged, replacements are available from BioTek. The ELx800 and its accessories are securely packaged inside custom-designed shipping materials. This packaging should protect the instrument from damage during shipping. Inspect the shipping box, packaging, instrument, and accessories for signs of damage.

  • Page 39: Unpacking The Instrument And Its Accessories

    Unpacking and Inspecting the ELx800™ | Unpacking the Instrument and Its Accessories Carefully open the top of the box, and remove the power supply shelf Figure 1 Remove the manual, Declaration of Conformity, and other accessories. Power supply Shelf Figure 1: Removing the power supply and shelf...

  • Page 40: Figure 2: Removing The Foam End Caps

    ). Place the reader on a level surface and remove the instrument from the 2-Mil plastic bag. Unpacking instructions Left end cap Unit inside 26 x 32 2-Mil poly bag Right end cap Figure 2: Removing the foam end caps BioTek Instruments, Inc.

  • Page 41: Figure 3: Removing The Top Cover Mounting Screws

    Unpacking and Inspecting the ELx800™ | Carefully turn the ELx800 upside down on a level surface. Use the slotted screwdriver to remove the four mounting screws from the top cover ( Figure 3 ) and unhook the shipping straps from the post on the bottom of the instrument.

  • Page 42: Figure 4: Removing The Shipping Block

    Replace the top cover: turn the instrument upside down and use the slotted screwdriver to replace the cover’s four mounting screws. Remove the shipping straps from the top of the carrier and store them with the packaging materials. Turn the instrument right side up. BioTek Instruments, Inc.

  • Page 43: Setting Up The Elx800

    Setting Up the ELx800 Operating Environment The ELx800™ is designed to operate optimally when installed on a level surface in an area where ambient temperatures remain between 18°C (64°F) and 40°C (104°F). The reader is sensitive to extreme environmental conditions, and these conditions should be avoided: •...

  • Page 44: Power-Up And System Test

    Chapter 2: Installation Power-Up and System Test After you have installed the ELx800™ and connected the power supply, turn on the instrument to run a system test. The on/off switch is located on the lower right side of the base.

  • Page 45: Configuring Global Default Options

    Setting Up the ELx800 | Configuring Global Default Options The ELx800™ contains several global configurable options, such as date and time, report output, and plate reading preferences. These options are accessed via the Select Utility Option menu ( Figure 5 below), and include: •...

  • Page 46: Connecting A Printer To The Elx800

    Place the printer in a location adjacent to the ELx800. Attach one end of the cable to the parallel port on the printer. Attach the other end of the cable to the parallel port on the ELx800. Tighten the securing screws on both ends of the cable.

  • Page 47: Figure 6: Connectors For Printer (Parallel), Computer (Serial), And Power

    Setting Up the ELx800 | Serial port Parallel port (printer port) Power supply jack Figure 6: Connectors for printer (parallel), computer (serial), and power supply ELx800 Operator’s Manual...

  • Page 48: Setting Up The Serial Port For Communications With Other Devices

    (such as a host PC running BioTek’s Gen5™, KCjunior™ or KC4™ software), the communication parameters must match between the devices. The ELx800 has a 25-pin serial (RS-232) port located on the rear panel of the instrument. The serial port allows the reader to communicate with a computer, using standard communications software and/or RS-232 protocols.
  • Page 49 To change the baud rate (or other communications settings) in KC4™ or KCjunior™ software, refer to the KC4 or KCjunior User Guides, or to Appendix . In Gen5™ software, the baud rate for the ELx800 is set at A, Computer Control 9600 and cannot be changed.

  • Page 50: Installing Additional Filters

    Installing Additional Filters Installed in the internal, five-position filter wheel are the filters that come standard with the ELx800™ (standard models have 405, 450, 490, 630 nm filters; the UV model’s filter set is 405, 450, 490, 630 and 340 nm).
  • Page 51 Connect the power supply and cable to the rear of the instrument. Important! Store unused filters in a cool, dry place away from direct sunlight. The filters can be wrapped in a piece of lens paper to protect them from scratches and dust accumulation. ELx800 Operator’s Manual...

  • Page 52: Checking The Reader's Filter Table Setting

    Chapter 2: Installation Checking the Reader’s Filter Table Setting After installing new filters, ensure that the ELx800’s filter table (the reader’s software reference for filter locations) matches the physical location of the filters. To check or change the software filter table: Power up the reader.
  • Page 53 I N F O R M A T I O N : D A T E T I M E F I L T E R * M O R E Press the Main Menu key to return to the main menu. ELx800 Operator’s Manual...

  • Page 54: Repackaging And Shipping The Elx800

    If you need to ship the ELx800™ Reader to BioTek for service or repair, be sure to use the original packing. Other forms of commercially available packing are not recommended and can void the warranty.

  • Page 55: Repackaging The Elx800 And Its Accessories

    Repackaging and Shipping the ELx800 | Repackaging the ELx800 and Its Accessories Refer to through when repackaging the ELx800™. Figures 8 Move the carrier to the home position. Turn off the instrument and unplug the power supply. Wrap the shipping straps around the carrier (...

  • Page 56: Figure 9: Hooking The Shipping Straps Over The Post

    Using a slotted screwdriver, remove the four top cover mounting screws. Lift the instrument off the cover and turn it over. Shipping straps Mounting screws Post Top cover Figure 9: Hooking the shipping straps over the post BioTek Instruments, Inc.

  • Page 57: Figure 10: Installing The Shipping Block

    Repackaging and Shipping the ELx800 | Install the shipping block: place the groove in the shipping block over the shaft and use a Phillips screwdriver to attach the two mounting screws Figure 10 Shipping block Shaft Figure 10: Installing the shipping block...

  • Page 58: Figure 11: Reinstalling The Top Cover

    Chapter 2: Installation Turn the instrument over and install the top cover that was removed in step 6 ( Figure 11 Mounting screws Top cover Figure 11: Reinstalling the top cover BioTek Instruments, Inc.

  • Page 59: Figure 12: Reattaching The End Caps

    Repackaging and Shipping the ELx800 | Turn the instrument right side up, place it in the 2-Mil plastic bag, then attach the left and right end caps ( Figure 12 Left end cap 26 x 32 2-Mil poly bag Right...

  • Page 60: Figure 13: Placing The Unit In The Box

    Place the shelf into the notched-out area in the end cap, and the power supply into an 8” x 11 ½” bubble bag. Place the power supply on the shelf. Figure 13 Shelf Power supply Figure 13: Placing the unit in the box BioTek Instruments, Inc.

  • Page 61: Preparing The Shipping Container

    Make a note of any error messages displayed and their frequency. Provide BioTek with the name and contact information of a person who may be contacted if questions arise. Close the box and tape it shut.
  • Page 62 Chapter 2: Installation BioTek Instruments, Inc.

  • Page 63: Chapter 3: Operation

    Chapter 3 Operation This chapter includes instructions for operating the ELx800™ and its software. ELx800 Front Panel ..............38 Overview ................40 Recommendations for Achieving Optimum Performance....40 System Startup ..............41 Main Menu ................42 Define ................... 44 Define (Method, Map, Formula and Curve)........47 Defining METHOD ...............

  • Page 64: Elx800 Front Panel

    Chapter 3: Operation ELx800 Front Panel R E A D Y 9 : 4 5 A M 0 9 / 1 2 / 0 4 R E A D D E F I N E R E P O R T...
  • Page 65 ELx800 Front Panel | The keypad has four one below each selectable menu SOFT KEYS, option. Press a SOFT KEY to make a selection. For example, from the Main Menu, press the leftmost SOFT KEY to select READ, the rightmost to select UTIL.

  • Page 66: Overview

    The ELx800™ features a 25-pad keypad and a 2-line x 24-character LCD display, allowing you to access the reader’s program menus and print test results. The reader’s bidirectional serial port allows computer control of the instrument, and provides the means for downloading additional assay definition files to the instrument.

  • Page 67: System Startup

    System Startup | System Startup To turn on the ELx800™, press the on/off switch on the right side of the reader’s base. The ELx800 will perform a System Test, displaying the screens shown below until initialization is complete. During this period, all keys are inactive.

  • Page 68: Main Menu

    Chapter 3: Operation Main Menu Following successful power-up of the ELx800™, the Main Menu appears: R E A D Y 9 : 4 5 A M 0 1 / 3 1 / 0 3 R E A D D E F I N E...
  • Page 69 Single Wavelength 405 nm (editable) • 96-well plate geometry • Blank on Air • Automap • Map starting location A1 • Samples only (no blanks, standards or controls) • Sample count prompted at runtime (can be turned off in UTIL|READ options) ELx800 Operator’s Manual...

  • Page 70: Define

    Specify a curve fit type and x/y axis types (lin/log). Specify whether or not standard outliers can be edited, and then the method by which they will be edited. Enable or disable the extrapolation feature (page 79). BioTek Instruments, Inc.

  • Page 71: Programming Note

    C U R V E - F I T T Y P E : N O N E N O N E L I N E A R Q U A D * M O R E ELx800 Operator’s Manual...

  • Page 72: Selecting An Assay To Define

    The cursor is positioned at the first editable field, and advances automatically. The numeric range depends on the number of assays stored in the reader’s memory. The ELx800 has 55 “open” assays available; the EL800 has only 10. Press ENTER to advance to the EDIT ASSAY NAME screen.

  • Page 73: Define (Method, Map, Formula And Curve)

    The options appear on the display in the order that they were programmed in the assay. If the assay contains a closed variable (i.e., an element of the assay definition that you cannot access or modify), the entry screen is skipped. ELx800 Operator’s Manual...
  • Page 74 4 5 0 4 9 0 6 3 0 Select the wavelength. Use the right arrow key to move the cursor to REF and then select the Reference Filter. Press ENTER to move to the next screen. BioTek Instruments, Inc.
  • Page 75 Plate Type For 6- to 384-well standard plates, the plate types and sizes included in the software onboard the ELx800™ are based on the brands listed below. For best measurement results, use these brands when operating the ELx800 via its onboard software.
  • Page 76 6-well (2 x 3) 96-well (8 x 12) 12-well (3 x 4) 96-well Hellma Quartz (8 x 12) 96H: 24-well (4 x 6) 96-well Metric (8 x 12, 9 mm well 96M: 48-well (6 x 8) spacing) BioTek Instruments, Inc.

  • Page 77: Defining Map

    The 384-well plate type is preset for the map to have 384 samples. This does not include any blanking, controls, or standards. (Selecting 6-, 12-, 24-, 48-, or 96-well geometries enables data reduction capabilities.) ELx800 Operator’s Manual...
  • Page 78 Select MANUAL PLATE MAP GENERATION to indicate that the well assignments will be performed manually (by the user) at Define and/or Read time. • Press ENTER to save the selection and continue. Use the SHIFT-CLEAR keys to clear any previously defined manual map. BioTek Instruments, Inc.
  • Page 79 A1 and A2 wells. The third replicate would follow in B1. The next standard control, or sample, would follow in B2. • Press ENTER to save the selection and continue. Examples of mapping directions are shown on the next page. ELx800 Operator’s Manual...
  • Page 80 STD3 STD4 STD4 STD5 STD5 Map Direction DOWN, Rep Direction ACROSS: STD1 STD1 STD2 STD2 STD3 STD3 STD4 STD4 STD5 STD5 Map Direction ACROSS, Rep Direction DOWN: STD1 STD2 STD3 STD4 STD5 STD1 STD2 STD3 STD4 STD5 BioTek Instruments, Inc.
  • Page 81 A01 to the last well on the plate, depending on the plate type and the number of blanks, standards, controls, and/or samples defined in the assay. • Press ENTER to save the well location and continue. ELx800 Operator’s Manual...
  • Page 82 Select the BLANK MAP type (see the descriptions on the next page). • Press *MORE to cycle through the available options: ROW or COLUMN, and P-ACROSS or P-DOWN. • Press ENTER to save the well location and continue. BioTek Instruments, Inc.
  • Page 83 A, C, E and G rows. Manual mapping is recommended to set up the appropriate map by placing the standards, controls, and samples in only the A, C, E, and G rows. ELx800 Operator’s Manual...
  • Page 84 B L A N K S : • Use the numeric keys to enter the number of blanks. The range is 0 to • Use the CLEAR key to clear the NUMBER OF BLANKS value from the display. • Press ENTER to continue. BioTek Instruments, Inc.
  • Page 85 12. The minimum is 4 for 4-P fit, cubic, cubic spline, and logit-log; 3 for quadratic; and 2 for linear and point-to-point. • Press CLEAR to clear the value on the display. • Press ENTER to continue. ELx800 Operator’s Manual...
  • Page 86 Select YES to average the replicates for each standard group, and then use the group averages when calculating the standard curve. • Select NO to use the individual standard replicates when calculating the standard curve. • Press ENTER to continue. BioTek Instruments, Inc.
  • Page 87 The following table lists acceptable entries for well locations based on plate geometry: Plate Type Range 6-Well A01-B03 12-Well A01-C04 24-Well A01-D06 48-Well A01-F08 96-Well A01-H12 A01-H12 A01-H12 ELx800 Operator’s Manual...
  • Page 88 Chapter 3: Operation Reuse of Standard Curves The ELx800™ has the ability to reuse a standard curve that has already been established. Limitations of the Reuse of Standard Curves Standard curves can only be reused in assay positions 31 through 55.
  • Page 89 For example, if the assay requires one or more positive control wells and one or more negative control wells, enter 02. • The valid entry range depends on the number of locations on the plate that are undefined. The maximum number of control groups is 8. ELx800 Operator’s Manual...
  • Page 90 The valid entry range is from 1 to 12 replicates. The software automatically performs a check to ensure the number of replicates, multiplied by the number of controls, does not exceed the number of undefined wells remaining on the plate. BioTek Instruments, Inc.
  • Page 91 The range is 0 to the number of undefined well locations remaining on the plate. For example, if there are no controls, blanks, or standards defined on a 96-well plate, the maximum number of samples is 96, and the minimum number of samples is 1. ELx800 Operator’s Manual...
  • Page 92 R E P # 1 : C 0 2 • Use the numeric alpha keys to enter the well location for Rep #1 of Sample Group #1. Press ENTER to advance to the next replicate or sample group. BioTek Instruments, Inc.

  • Page 93: Defining Formula

    KCjunior™. Gen5, KC4, and KCjunior are PC-based software programs you can use to set up your assay, communicate with the ELx800 to run the assay, and then manipulate the raw data that is automatically retrieved from the reader. Refer to Appendix B for additional information.
  • Page 94 Chapter 3: Operation Formula Type The ELx800™ supports three types of formulas, as well as the ability to define variables for use within Transformation formulas. Note: GENERAL formulas are not used. S E L E C T F O R M U L A...
  • Page 95 Select FUNCTN to insert a mathematical function such as LOG or SQRT. The reader software checks the formulas for errors during data reduction. A syntax error in a formula will result in a “Token Error” on results reports. ELx800 Operator’s Manual...
  • Page 96 Log10 2 = 0.301029995 ALOG10- Anti Log Base 10- LOG- Log- LOG 2 = 0.69314718 ALOG10 (0.30102995) = 2 AB- Absolute Value- AB (-1) = 1 SQRT- Square Root- SQRT 2 = 1.4142 PWR- Power- (10 PWR 2) = 100 BioTek Instruments, Inc.
  • Page 97 2.500, this can be accomplished with one formula: PC>1.000ANDPC<2.500 Or with two separate formulas: PC > 1.000 and PC < 2.500 Negative Control mean must have an OD of less than 0.100, the formula is: NC;x < 0.100 ELx800 Operator’s Manual...
  • Page 98 If an assay protocol states that for the assay to be valid: The mean of the negative controls must be less than 0.100. The formula is: NC;x<0.100 The mean of the positive controls must be greater than the mean of the negative controls. The formula is: PC;x>NC;x BioTek Instruments, Inc.
  • Page 99 (OD), or just to the sample wells (SMP). S C O P E V A R I A B L E : S M P ELx800 Operator’s Manual...
  • Page 100 - Subtract the mean of CTL1 from the mean of the NC. Subtract the difference from all ODs on the plate. - Divide the result of the above by the mean of the NC less the mean of CTL1, and then multiply by 100. BioTek Instruments, Inc.
  • Page 101 ENTER Enter STD1 as the TVAR formula. Press ENTER The formula selection screen is displayed. Choose TRANS. Enter the formula (OD/TVAR)*100 using the MATH, OTHER, MAP, and FUNCTION keys. “TVAR” is available as a MAP option. ELx800 Operator’s Manual...
  • Page 102 The valid entry range is from 00 to 99%. An entry of 00% indicates no greyzone, although a sample equal to the cutoff value will still receive the EQUIV call. on the next page for information on Positive / Negative Calls how calls are assigned. BioTek Instruments, Inc.
  • Page 103 The greyzone is 00% • is selected for SAMP>CUTOFF+00% • Calls are assigned to sample wells as follows: EQUIV if the sample equals 1.500 POS if the sample is greater than 1.500 NEG if the sample is less than 1.500 ELx800 Operator’s Manual...
  • Page 104 Calls are assigned to sample wells as follows: EQUIV if the sample is greater than or equal to 1.800 and less than or equal to 2.200 POS if the sample is greater than 2.200 NEG if the sample is less than 1.800 BioTek Instruments, Inc.

  • Page 105: Defining Curve

    • Extrapolation of Unknowns Note: These screens are displayed on the ELx800 in the order in which they appear in the assay. If a closed variable (i.e., an element of the assay definition that you cannot access or modify) is being used in the assay, the entry screen is omitted.
  • Page 106 Press SOFT KEYS 1, 2, 3, or 4 to select the curve-fit type that is displayed above the soft key. Select *MORE to display additional options. • Press ENTER to save the selection and advance to the next screen. BioTek Instruments, Inc.
  • Page 107 After the assay is run and reports are generated, press REPORT from the Main Menu. Press RESULT, select the assay, and then press ENTER . The EDIT STD OUTLIERS? YES/NO prompt will appear. on page 92 for further Editing Standard Outliers instructions. ELx800 Operator’s Manual...
  • Page 108 “Ambiguous.” The resulting values, which actually are extrapolated, may not be indicated as such. All calculated results for an “Ambiguous” curve should be considered unreliable. BioTek Instruments, Inc.
  • Page 109 The type and number of controls, blanks, standards, and replicates in the assays chosen for the Panel will be “copied” into the Panel definition. Map or assay parameters must first be changed in the predefined assay before they can change in the Panel. ELx800 Operator’s Manual...
  • Page 110 Press Options to cycle through the assay numbers and names, or use numeric keys to enter an assay number. Press ENTER to make a selection. • After an assay is selected, its starting location must be defined. BioTek Instruments, Inc.
  • Page 111 Assay 8 mapping is complete. The Panel Assay results are sorted by sample (unless a custom assay has been programmed by BioTek). Note: The interpretation of Results reports for each assay in the Panel will print first, and then the Sample results will print.

  • Page 112: Reading A Microplate

    S P A C E E N T E R S A M P L E I D : 0 0 0 1 Place the plate in the carrier, and then press the READ key to continue. BioTek Instruments, Inc.

  • Page 113: Selecting An Assay To Run

    , whether or not UTIL READ the assay specifies manual mapping, or if the assay was created or downloaded from BioTek’s Extensions™ Define Reader Protocol software. • Prompts enabled via can include ENTER NUMBER OF UTIL READ SAMPLES, PLATE ID, and ENTER SAMPLE ID.
  • Page 114 Use caution when creating multiple Plate IDs. The reader does not warn you that you are about to exceed the maximum of 8 plate IDs stored in memory. If a ninth Plate ID is added, it will overwrite the first Plate ID stored in memory. BioTek Instruments, Inc.
  • Page 115 • The sample well locations originally defined in the assay will be presented. If desired, use the keypad to enter new well locations for each sample replicate. • Press ENTER again to advance to the next replicate. ELx800 Operator’s Manual...
  • Page 116 Place the plate in the carrier and press the READ key to initiate the plate read. • After the read is complete, data reduction will be performed (“Calculating Results…”), and then the reports will print (“Generating Reports…”). • To halt the read in progress, press the STOP key. BioTek Instruments, Inc.

  • Page 117: Printing Reports

    Select ASSAY to print a plate map and a listing of all of the assay’s settings, such as wavelengths, numbers of well types, formulas, and curve-fit parameters. • Select LIST to print a list of all assays (name and number) currently programmed in the ELx800™. ELx800 Operator’s Manual...

  • Page 118: Editing Standard Outliers

    Select YES to indicate that one or more standard replicates or groups should be temporarily excluded from curve-fit calculations. If AVERAGE STANDARDS was set to NO in the assay definition, one or more standard replicates can be chosen for exclusion. BioTek Instruments, Inc.
  • Page 119 4 for 2-P, 4-P, cubic, and cubic-spline; 3 for quadratic; and 2 for linear and point-to- point. Exercise caution when editing outliers. If the assay is left with insufficient standards, the curve fit will fail. ELx800 Operator’s Manual...

  • Page 120: Printing Results

    List Report The List Report lists the all of the assays (name and number), currently programmed on the reader. • Select REPORT from the Main Menu, and then select LIST to print the report. BioTek Instruments, Inc.

  • Page 121: Using The Utility Options

    To change the time format, select 12HOUR or 24HOUR, then AM or PM. The display automatically updates to reflect the new format. Press ENTER to return to the SELECT UTILITY OPTION menu. ELx800 Operator’s Manual...

  • Page 122: Viewing/Editing The Filter Table

    Chapter 3: Operation Viewing/Editing the Filter Table After installing new filters, ensure that the ELx800’s filter table (the reader’s software reference for filter locations) matches the physical location of the filters in the filter wheel. To edit the filter table: From the Main Menu, select UTIL SETUP.

  • Page 123: Specifying Data Output And Reporting Options

    E P S O N E P S O N The ELx800 supports printers using either HP's PCL3 language, such as the HP DeskJet series, or Epson's LQ language. For the latest list of compatible printers, consult the BioTek Web site (www.biotek.com), or call BioTek Instruments' Technical Assistance Center (refer to Chapter 1 for contact information).
  • Page 124 P R I N T C U R V E - F I T ? Y E S Select YES to print the standard curve (only applies to quantitative assays). Press ENTER to return to the SELECT UTILITY OPTION screen. BioTek Instruments, Inc.

  • Page 125: Selecting Read Options

    (single-wavelength assay). If NO is selected, the plate will be read in (approximately 50 seconds). Refer to Chapter 1 for NORMAL MODE specifications. Pressing ENTER after each selection advances the display. When selections are completed, the display returns to the SELECT UTILITY OPTION menu. ELx800 Operator’s Manual...
  • Page 126 Chapter 3: Operation BioTek Instruments, Inc.

  • Page 127: Chapter 4: Instrument Qualification

    Chapter 4 Instrument Qualification This chapter discusses the tasks and procedures necessary for verifying and qualifying instrument performance on an ongoing basis. A convenient Recommended Qualification Schedule arranges tasks into Installation, Operational, and Performance Qualification categories. Recommendations for Achieving Optimum Performance....102 Recommended Qualification Schedule ........

  • Page 128: Recommendations For Achieving Optimum Performance

    Filter solutions to remove particulates that could cause erroneous readings. Although the ELx800™ supports standard flat, U-bottom, and V-bottom microplates, optimum performance is achieved with optically clear, flat-bottomed wells. Nonuniformity in the optical density of the well bottoms can cause loss of accuracy, especially with U- and V-bottom polyvinyl microplates.

  • Page 129: Recommended Qualification Schedule

    * If you have an Absorbance Test Plate, run Liquid Test 1. If you do not have an Absorbance Test Plate, run Liquid Test 2. ** Liquid Test 3 is optional; it is provided for sites requiring verification at wavelengths lower than those attainable with the Absorbance Test Plate. ELx800 Operator's Manual...

  • Page 130: Qualification Procedures

    Qualification (IQ/OQ/PQ) package (PN 7330538) for the ELx800 is available for purchase. Contact your local dealer for more information. Your ELx800 reader was fully tested at BioTek before shipment and should operate properly upon initial setup. If you suspect that problems may have occurred during shipment, if you reshipped the instrument, or if regulatory requirements dictate that Performance Qualification Testing is necessary, you should perform the following tests.

  • Page 131: System Test

    The reader will “chirp” repeatedly if the power-on System Test results do not meet the internally coded Failure Mode Effects Analysis (FMEA) criteria established by BioTek. A system test should then be initiated to try to retrieve an error code from the reader.
  • Page 132 Upper Right Corner: x= 544 y=10738 Delta 1: 9226 - 9224= Delta 2: 544 - 542= Delta 3: 10738 –10746= Delta 4: 16258 –16268= -10 SYSTEM TEST PASS -000 Figure 16: Sample output for the System Test BioTek Instruments, Inc.

  • Page 133: Checksum Test

    7 3 3 0 2 9 1 - F W V 1 . 0 0 . 0 The second checksum test display will show the assay configuration software part number and version number. After a few moments, the Main Menu will reappear. ELx800 Operator's Manual...

  • Page 134: Absorbance Plate Test

    A reading of more than 0.015 OD for any of the designated alignment wells indicates that the light is being “clipped” and the ELx800 may be out of alignment. If the reader fails the alignment test, review the following possible problems and solutions: Ensure that the Test Plate is correctly seated in the microplate carrier, with “A1”...
  • Page 135 Check the filters on the test plate to ensure that there is no debris that may have shifted between readings and caused changes. Check the microplate carrier to ensure that it is clear of debris. If these remedies are ineffective, call your BioTek representative or BioTek Technical Support. ELx800 Operator's Manual...

  • Page 136: Requirements

    Chapter 4: Instrument Qualification Requirements To run the Absorbance Plate Test, you need BioTek's Absorbance Test Plate (PN 9000547 or PN 7260522), with its accompanying Standards Certificate, shown Figure 18 Figure 19 shows a sample Test Plate Analysis Report. This test plate can be used for testing the reproducibility, linearity, and alignment of your BioTek autoreader.

  • Page 137: Defining The Absorbance Test Plate Parameters

    Enter the values listed on the Test Plate Standards Certificate. After each entry, press ENTER to advance to the next consecutive well location. Repeat for the remaining filters. When all values have been entered, press the Main Menu key. ELx800 Operator's Manual...

  • Page 138: Running The Absorbance Plate Test

    ENTER C A L I B R A T I O N F I L T E R : 4 0 5 4 0 5 4 5 0 4 9 0 * M O R E BioTek Instruments, Inc.

  • Page 139: Test Failures

    If any of the test parameters report as "FAIL," confirm that the standard values on the test plate Standards Certificate match the values on the printout. If not, correct and retest. If the test still fails, contact BioTek's Technical Assistance Center (refer Chapter 1 for contact information).

  • Page 140: Liquid Testing

    (see page 123). • BioTek offers a dye solution (PN 7120779, 25 ml; or 7120782, 125 ml) that may be used in the stock solution formulation for Liquid Tests 1 and 2, or, if you prefer, you may use the dye solution described in Table 3 (page 119).

  • Page 141: Stock Solution Formulation

    Rinse the contents into a 1-liter volumetric flask. Add 0.5 ml of Tween 20, or 5 ml of BioTek’s wetting agent. Make up to 1 liter with DI water, cap, and shake well. This should create a solution with an absorbance of about 2.000 when using 200 µl in a flat-bottom microwell.
  • Page 142 • 100-ml volumetric flask Preparation of Stock Solution: Pipette a 5-ml aliquot of BioTek QC Check Solution No. 1 into a 100-ml volumetric flask. Make up to 100 ml with DI water; cap and shake well. This should create a solution with an absorbance of about 2.000 when using 200 µl in a flat-bottom microwell.

  • Page 143: Liquid Test 1

    Print the five sets of raw data or export it to an Excel spreadsheet using Gen5™, KC4™, or KCjunior™. If you are exporting the data to your own Excel spreadsheet, perform the calculations described below and keep the spreadsheet for future tests. ELx800 Operator's Manual...

  • Page 144: Table 3: Test Tube Dilutions

    Repeatability Specification: For comparison in this test, the following repeatability specification is applied, using the Normal Read Mode and a 96-well microplate. ± 0.5% ± 0.005 OD from 0.000 to 2.000 OD @ 405 nm BioTek Instruments, Inc.

  • Page 145: Liquid Test 2

    1, 90% of the concentrated solution in tube 2, 80% in tube 3, and so on to 10% in tube 10. Dilute using amounts of the remaining 0.05% solution of deionized water and Tween 20, as shown in Table 3 ® ELx800 Operator's Manual...
  • Page 146 Perform a regression analysis on the data to evaluate linearity. For example, using Microsoft Excel: ® In a spreadsheet, create two columns labeled X and Y. Enter the actual absorbance values in column X. Enter the expected absorbance values in column Y. BioTek Instruments, Inc.
  • Page 147 Since the standard deviation is less than this value, the reader meets the test criteria. Repeatability Specification: For comparison in this test, the following repeatability spec is applied, using Normal mode, 96-well microplate: ± 0.5% ± 0.005 OD from 0.000 to 2.000 OD @405 nm ELx800 Operator's Manual...
  • Page 148 If the four corner wells are within the accuracy range, the reader is in alignment. Accuracy Specifications: For comparison in this test, the following accuracy specification is applied, using Normal Mode and a 96-well microplate: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm BioTek Instruments, Inc.

  • Page 149: Liquid Test 3 (For "Uv" Models Only)

    Liquid Test 3 at wavelengths lower than those attainable with the Absorbance Test Plate. This test verifies operation of the ELx800™ at 340 nm and is optional because the reader has good “front-end” linearity throughout its wavelength range. Required Materials: •...
  • Page 150 1. Prepare a PBS solution using the Sigma tablets. 2. In a beaker, mix 50 ml of the PBS solution (prepared from the Sigma tablets) with 10 mg of the -NADH powder and mix thoroughly. This is the Sigma PBS Solution BioTek Instruments, Inc.
  • Page 151 Print the five sets of raw data or export it to an Excel spreadsheet using Gen5™, KC4™, or KCjunior™. If you are exporting the data to an Excel spreadsheet, perform the calculations described on the following page, and keep the spreadsheet for future tests. ELx800 Operator's Manual...
  • Page 152 Analysis ToolPak in Microsoft Excel. Consult Excel’s help system for assistance. Expected Results: Since it is somewhat difficult to achieve high pipetting accuracy when conducting linear dilutions, an R-Square value greater than or equal to 0.990 is considered adequate. BioTek Instruments, Inc.

  • Page 153: Chapter 5: Maintenance And Decontamination

    Chapter 5 Maintenance and Decontamination This chapter contains procedures for maintaining and decontaminating the ELx800™. Maintenance ................128 Routine Cleaning Procedure............128 Filter Storage and Handling ............129 Replacing the Bulb ..............130 Decontamination Procedure............133...

  • Page 154: Maintenance

    Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Do not soak the keypad—this will cause damage. Moisten a clean cloth with deionized or distilled water and wipe the keypad.

  • Page 155: Tools And Supplies

    Use a clean, dry cloth to dry all wet surfaces. Filter Storage and Handling To properly store interference filters for the ELx800™ microplate reader during extended periods of non-use, package the filters in a light-tight envelope or container, away from high humidity.

  • Page 156: Replacing The Bulb

    *MORE until BULB ALIGN is displayed. The carrier will move to the bulb alignment position. E D I T S E T U P B U L B A L I G N * M O R E BioTek Instruments, Inc.

  • Page 157: Figure 20: Lamp Assembly

    Replacing the Bulb | Bulb assembly Bulb retainer Optics arm Filter wheel cover Lamp retention screws Lead wires Optics arm cover Optics arm #1 aperture Lamp retention screws Figure 20: Lamp assembly ELx800 Operator's Manual...
  • Page 158 Tighten the lamp retention screws and verify that the lamp has not moved. After completing the alignment, turn the instrument off and on again to return the carrier to the home position. BioTek Instruments, Inc.

  • Page 159: Decontamination Procedure

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle.

  • Page 160: Tools And Supplies

    “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Do not soak the keypad—this will cause damage. Important! Turn off and unplug the instrument for all decontamination and cleaning operations.
  • Page 161 7. Use a clean, dry cloth to dry all wet surfaces. 8. Discard the used gloves and cloths using a Biohazard trash bag and an approved Biohazard container. ELx800 Operator's Manual...
  • Page 162 Chapter 5: Maintenance and Decontamination BioTek Instruments, Inc.

  • Page 163: Chapter 6: Troubleshooting And Error Codes

    Chapter 6 Troubleshooting and Error Codes This appendix describes potential error codes that may be displayed on the ELx800™, and suggests possible solutions for these problems. Overview ................138 System Test Description ............138 Glossary of Terms ...............138 Error Codes ................139 General Errors ..............140...

  • Page 164: Overview

    If you cannot resolve the problem, run the instrument System Test and note the exact error code and serial number of the reader. Then call BioTek’s Technical Assistance Center (refer to Chapter 1 for contact information). System Test Description...

  • Page 165: Error Codes

    See Fatal Errors on page 146 for more information. Contact BioTek's Technical Assistance Center for further assistance if any of error codes are displayed. Refer to Chapter 1 for contact information.

  • Page 166: General Errors

    Flex cable is disconnected or damaged. • X-axis movement is limited so that the optical sensor cannot be interrupted. Note: In cases where a sensor is not functioning, the motor will drive the axis to its mechanical stop and generate substantial noise. BioTek Instruments, Inc.
  • Page 167 • Failed lamp. • Loose filter wheel drive gear or defective motor controller PCB. 0302 Filter wheel failed positional verify; lamp failed • A failed lamp generates a 0302 error on power-up. ELx800 Operator's Manual...
  • Page 168 Bearings caused motors to fail. 0500 Filter not installed Probable Causes: • Empty filter location on the filter wheel (all filter locations must have either a filter or a filter blank, BioTek PN 2872086). • Entire filter wheel is not installed. BioTek Instruments, Inc.
  • Page 169 There may be an ambient light leak. Ensure that the plate carrier door is properly closed. Faulty motherboard (PN 7330400, 7330410, or 7330415) or daughter board (PN 7330404, or 7330414). Photodiode or power supply may be too close to the instrument. ELx800 Operator's Manual...
  • Page 170 Printer timed out The time allotted for the instrument to make a valid connection to a printer has expired. Probable Causes: Printer not connected, on line, or powered up. • • Printer parallel port may not be correctly selected. BioTek Instruments, Inc.
  • Page 171 Error Codes | General Errors (Continued) Errors 0D00 through 1300 indicate that the reader has failed one or more of a number of self-tests. Contact BioTek’s Technical Assistance Center for instructions. Refer to Chapter 1 for contact information. 0D00 Calibration checksum error Reader failed calibration checksum test.

  • Page 172: Fatal Errors

    Probable Causes these errors on pages 140 and 141. Fatal Errors Fatal Errors indicate conditions that require immediate attention. If a fatal error is displayed, contact BioTek’s Technical Assistance Center for further instructions. Refer to Chapter 1 for contact information.

  • Page 173: Appendix A: Computer Control

    Appendix A Computer Control The ELx800™ can be controlled with BioTek’s Gen5™, KC4™, or KCjunior™ software. This Appendix provides basic instructions for installing and configuring Gen5, KC4, or KCjunior software for control of the reader. Overview ................148 Controlling the Reader With Gen5™........... 149 Controlling the Reader With KC4™...

  • Page 174: Overview

    This Appendix provides basic instructions for installation and configuration of BioTek’s Gen5, KC4, or KCjunior software for control of the ELx800. Instructions are also provided for reading a plate using these software packages. For more detailed instructions, refer to the Gen5 Getting Started Guide or Help system, or to the KC4 or KCjunior User’s Guides.

  • Page 175: Controlling The Reader With Gen5

    Guide. Setting Up Gen5 The ELx800™ can be operated via BioTek's Gen5 software installed on a host computer. The following instructions briefly show you how to set up Gen5 for operation of the reader. Refer to Gen5’s Getting Started Guide or Help system for more detailed instructions.

  • Page 176: Problems

    ( File|New Protocol Refer to Gen5’s Help system early and often to learn how to create protocols, assign well identifiers, read plates, print reports, perform data reduction, and more. BioTek Instruments, Inc.
  • Page 177 The results will be displayed for the selected wavelength. Repeat, for other wavelengths. To analyze, manipulate, or print results, Protocol parameters should be defined. Refer to Gen5’s Help system for instructions. Gen5 Reader Control does not support data reduction. ELx800 Operator's Manual...

  • Page 178: Controlling The Reader With Kc4

    Setting Up KC4 The ELx800™ can be operated via BioTek's KC4 software installed on a host computer. The following instructions briefly show you how to set up KC4 for operation of the reader. Refer to KC4’s User’s Guide or Help system for more detailed instructions.

  • Page 179: Problems

    The plate will be read and then the raw data results will display in KC4. To analyze, manipulate, or print results, Protocol parameters should be defined. Refer to KC4's Help system or User's Guide for instructions. ELx800 Operator's Manual...

  • Page 180: Controlling The Reader With Kcjunior

    Help system. Setting Up KCjunior The ELx800™ can be operated via BioTek's KCjunior software installed on a host computer. The following instructions briefly show you how to set up KCjunior for operation of the reader. Refer to KCjunior’s User’s Guide or Help system for more detailed instructions.

  • Page 181: Problems

    The plate will be read and then the raw data results will display in KCjunior. To analyze, manipulate, or print results, protocol parameters should be defined. Refer to KCjunior's Help system or User's Guide for instructions. ELx800 Operator's Manual...
  • Page 182 Appendix A: Computer Control BioTek Instruments, Inc.

  • Page 183: Appendix B: Using 384-Well Geometry

    Appendix B Using 384-Well Geometry When used in standalone mode, the ELx800NB™ does not have access to data reduction options for 384-well plate geometry. To capture and manipulate the raw data, you must use Gen5™, KC4™, or KCjunior™ software. This Appendix provides instructions for setting up and performing a 384-well plate read for data reduction in Gen5, KC4, and KCjunior.

  • Page 184: Perform A 384-Well Plate Read In Gen5

    Perform a 384-Well Plate Read in Gen5™ You will need the ELx800NB model to perform these instructions. (Other ELx800™ models do not support 384-well geometry.) Start Gen5. Log in, if prompted (Gen5 Secure users). When the ‘Welcome to Gen5’ screen appears, select System Menu.

  • Page 185: Figure 23: New Experiment Dialog

    . The ‘New Experiment’ dialog will appear: Figure 23: New Experiment dialog Click Default Protocol , then click . Gen5™ will open the Experiment workspace, which includes the Protocol menu and Plate screen. Figure 24: Experiment workspace ELx800 Operator’s Manual...

  • Page 186: Figure 25: Procedure Dialog

    Plate|Read or click the Read Plate icon. The ‘Procedure’ dialog will appear: Figure 25: Procedure dialog Select 384 WELL PLATE for the Plate Type Click Read to open the ‘Read Step’ dialog: Figure 26: Read Step dialog BioTek Instruments, Inc.

  • Page 187: Figure 27: Plate Reading Dialog

    The results will be displayed for the selected wavelength. Repeat, for other wavelengths. To analyze, manipulate, or print results, Protocol parameters should be defined. Refer to Gen5's Help system for instructions. Gen5 Reader Control does not support data reduction. ELx800 Operator’s Manual...

  • Page 188: Perform A 384-Well Plate Read In Kc4

    Appendix B: Using 384-Well Geometry Perform a 384-Well Plate Read in KC4™ You will need the ELx800NB model to perform these instructions. (Other ELx800™ models do not support 384-well geometry.) Start KC4. Login, if prompted. At KC4’s main screen, select...

  • Page 189: Figure 30: New Data File Dialog

    At the main screen, select Data|New Plate . The ‘New Data File’ dialog will appear: Figure 30: New Data File dialog Select Empty Protocol and click Select Protocol|Reading from the main screen to open the ‘Reading’ dialog: Figure 31: Reading dialog ELx800 Operator’s Manual...

  • Page 190: Figure 32: Plate Reading Dialog

    Results screen’s field to select the desired Data wavelength. To print the results, select Data|Print To analyze or manipulate results, Protocol parameters should be defined. Refer to KC4's Help system or User's Guide for instructions. BioTek Instruments, Inc.

  • Page 191: Perform A 384-Well Plate Read In Kcjunior

    Perform a 384-Well Plate Read in KCjunior™ | Perform a 384-Well Plate Read in KCjunior™ You will need the ELx800NB model to perform these instructions. (Other ELx800™ models do not support 384-well geometry.) Start KCjunior. At KCjunior’s main screen, choose...

  • Page 192: Figure 35: Read Plate Dialog

    KCjunior’s main screen. The ‘Read Plate Dialog’ will appear: Figure 35: Read Plate dialog If desired, enter the , then click Results ID Plate Description Read Plate The ‘Protocol Definition’ dialog will appear. Figure 36: Protocol Definition dialog BioTek Instruments, Inc.

  • Page 193: Figure 37: Example Read Method Parameters

    “Place the plate on the carrier.” The plate will be read and then the raw data results will display in KCjunior. To analyze or manipulate the results, Protocol parameters should be defined. Refer to KCjunior's Help system or User's Guide for instructions. ELx800 Operator’s Manual...
  • Page 194 Appendix B: Using 384-Well Geometry BioTek Instruments, Inc.

  • Page 195: Appendix C: Report Format

    Report Format This appendix contains examples of reports that can be generated and/or printed from the ELx800™. Refer to Printing Reports in Chapter 3 for details on how to print these reports. In addition, an Assay List, Assay Definition, Map, and Result can be printed by...

  • Page 196: Figure 38: Samples With Calls On Matrix Report

    Appendix C: Report Format Figure 38: Samples with calls on Matrix Report BioTek Instruments, Inc.

  • Page 197: Figure 39: Curve Fit Report

    Figure 39: Curve Fit Report ELx800 Operator's Manual...

  • Page 198: Figure 40: Samples With Calls On Column Report

    Appendix C: Report Format Figure 40: Samples with calls on Column Report BioTek Instruments, Inc.

  • Page 199: Figure 41: Column Report Without Samples

    Figure 41: Column Report without samples ELx800 Operator's Manual...

  • Page 200: Figure 42: Panel Report

    Appendix C: Report Format Figure 42: Panel Report BioTek Instruments, Inc.

  • Page 201: Figure 43: Assay Detail Report (Sheet 1 Of 2)

    Figure 43: Assay Detail Report (Sheet 1 of 2) ELx800 Operator's Manual...

  • Page 202: Figure 44: Assay Detail Report (Sheet 2 Of 2)

    A S S A Y A S S A Y A S S A Y A S S A Y A S S A Y A S S A Y Figure 44: Assay Detail Report (Sheet 2 of 2) BioTek Instruments, Inc.

  • Page 203: Appendix D: Comparison Of The Elx800 And The El800

    Appendix D Comparison of the ELx800 and the EL800 The following chart compares the features on the ELx800™ and EL800™ Readers. Feature ELx800 EL800 340-750 nm 405-750 nm Single- and dual-wavelength reading 6-well plates 12-well plates 24-well plates 48-well plates...
  • Page 204 Appendix D: Comparison of the ELx800 and the EL800 BioTek Instruments, Inc.

  • Page 205: Appendix E: Instructions For Programming A New Assay

    The kit instructions are provided so that users can see how it is possible to translate the kit wording into an ELx800™ assay program. For clarity, only the user menu choices from the reader screens are shown.

  • Page 206: Sample Ana Screen Enzyme Immunoassay Kit

    Phosphoric acid is added to each well to stop the reaction. This produces a yellow end-point color, which is read at 450 nm. BioTek Instruments, Inc.

  • Page 207: Materials

    This should be of the highest quality available. For dispensing 1000, 100, and 10 µl. • Calibrated Micropipettes: • Multichannel Pipette: Recommended for dispensing 100 µl volumes of conjugate, substrate, and stop solution. • Glass/Plastic Tubes: For sample dilution. ELx800 Operator's Manual...

  • Page 208: Quality Control And Results

    The ranges are provided as a guide only. ELISA assays are very sensitive and capable of detecting small differences in sample populations. It is recommended that each laboratory determine its own normal range, based on the population techniques and equipment employed. ANA Result Interpretation < 10.0 Negative > 10.0 Positive BioTek Instruments, Inc.

  • Page 209: Programming The Ana Screen Enzyme Immunoassay Kit

    Choose to blank on “AIR” if no blank wells are required NUMBER STDS: 00 NUMBER CTLS: 03 CONTROL 1: PC CONTROL 2: CTL1 Suggested choice for the cutoff control CONTROL 3: NC NUMBER OF REPLICATES PC: 01 CTL1: 03 ELx800 Operator's Manual...
  • Page 210 TRANS: FORMULA: (OD/TVAR)*10 Converts all OD values on plate to ”ANA Result” per kit insert instructions To define a cutoff formula for Positive and Negative calls: FORMULA CUTOFF: 10.0 GREYZONE: 00% SAMPLE > CUTOFF + 00%: POS BioTek Instruments, Inc.

  • Page 211: Sample Human Anticardiolipin Igg Enzyme Immunoassay Kit

    Phosphoric acid is added to each well to stop the reaction. This produces a yellow end-point color, which is read at 450 nm. ELx800 Operator's Manual...

  • Page 212: Materials

    1 bottle containing 12 ml of purified peroxidase labeled antibody. Colored red, ready to use. • TMB Substrate: 1 bottle containing 14 ml TMB substrate. Ready to use. • Stop Solution: 1 bottle containing 14 ml of 3M phosphoric acid. Ready to use. BioTek Instruments, Inc.

  • Page 213: Quality Control And Results

    Calculate Mean Optical Densities (for assays run in duplicate only) For each standard, control, and sample, calculate the mean OD of the duplicate readings. The user must verify that the percentage coefficient of variation (%CV) for each duplicate OD is less than 15.0%. ELx800 Operator's Manual...
  • Page 214 The Louisville reference center recommends the following positive discrimination criteria according to the recommendation of the 2 International Anticardiolipin Workshop. Criteria Range (GPLU/ml) High Positive > 80 Medium Positive ≥ 20-80 Low Positive ≥ 10, < 20 BioTek Instruments, Inc.

  • Page 215: Expected Values

    It is recommended that each laboratory determine its own normal range, based on the population techniques and equipment employed. IgG Anticardiolipin < 11 GPL U/ml Negative result > 11 GPL U/ml Positive result ELx800 Operator's Manual...

  • Page 216: Programming The Human Anticardiolipin Igg Enzyme Immunoassay Kit

    NUMBER STDS: 05 NUMBER STD REPLICATES: 01 CONCENTRATIONS: STD1: 6.25 STD2: 12.5 STD3: 25 STD4: 50 STD5: 100 NUMBER CTLS: 02 CONTROL 1: PC CONTROL 2: NC NUMBER OF REPLICATES PC: 01 NC: 01 SAMPLES: 89 SAMPLE REPLICATES: 01 BioTek Instruments, Inc.
  • Page 217 Kit instructions specify that samples Negative calls: with concentration values greater than 11 should appear as positive. The ELx800 software calculates the cutoff based on absorbance value or transformed value (see the previous example) and cannot calculate based on concentration. The technician must...
  • Page 218 Appendix E: Instructions for Programming a New Assay BioTek Instruments, Inc.

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