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Chapter 4: Instrument Qualification
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Repeatability Results
Wells
C1
Read 1
0.134
Min Limit
0.128
Max Limit
0.140
Read 2
0.135
Result
PASS
Reviewed/Approved By: _______________________________________
Date: ________________
For Technical Support
In the U.S.:
BioTek Instruments, Inc.
Tel: 800 242 4685
Fax: 802 655 3399
All Others:
Tel: 802 655 4040
Fax: 802 655 3399
email: TAC@biotek.com
Product support center: www.biotek.com/service
Results and Troubleshooting Tips
The Absorbance Test Plate Report contains results for the following:
•
Peak Absorbance:
test range(s) defined by the user in the Absorbance Test Plate dialog. To verify
wavelength accuracy, the wavelength of the maximum absorbance is compared
with the peak wavelength value entered in the software, which comes from the
data sheet supplied with the Test Plate. The accuracy of the wavelength should
be ± 3 nm (± 2 nm instrument, ± 1 nm filter allowance).
If the test fails:
E2
G3
0.566
1.037
0.555
1.022
0.577
1.052
0.566
1.037
PASS
PASS
In Europe:
BioTek Instruments GmbH
Tel: 49 (0) 7136-9680
Fax: 49 (0) 7136-968-111
When the test is performed, the C6 filter is scanned at the
Check the C6 filter to make sure it is clean. If needed, clean it with lens
paper.
Important!
Do not remove the filter from the Test Plate, and do
not use alcohol or other cleaning agents.
Make sure the information entered into Gen5 matches the information on
the Test Plate's data sheet.
Make sure the Test Plate is within its calibration certification period. The
calibration sticker is affixed directly to the plate. If it is out of date, contact
BioTek to schedule a recertification.
Check the microplate carrier to ensure it is clear of debris.
H6
F5
1.557
1.866
1.536
1.842
1.578
1.890
1.558
1.867
PASS
PASS
BioTek Instruments, Inc.
D4
2.385
2.308
2.462
2.385
PASS
Need help?
Do you have a question about the Synergy HT and is the answer not in the manual?
Questions and answers
I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you
To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:
1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.
If the issue persists, contact BioTek’s Technical Assistance Center.
This answer is automatically generated