Biotek 800TSI Instructions For Use Manual
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Biotek 800TSI Instructions For Use Manual

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800™ TS
Absorbance Reader
Instructions for Use
®
BioTek
Instruments, Inc.
© 2017
PN 1561011, Rev A

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Summary of Contents for Biotek 800TSI

  • Page 1 800™ TS Absorbance Reader Instructions for Use ® BioTek Instruments, Inc. © 2017 PN 1561011, Rev A...
  • Page 2 BioTek Instruments, Inc. Trademarks BioTek ® is a registered trademark, and 800™ TS and Gen5™ are trademarks of BioTek Instruments, Inc. BioCell™ is a trademark of BioTek Instruments and is patented under U.S. patent number 5,963,318.

  • Page 3: Contact Information

    Winooski, Vermont 05404-0998 USA Global Service and Support BioTek instrument service and repair is available worldwide at one of BioTek's International Service Centers and in the field at your location. To arrange for service or repair of your instrument, contact the office nearest you; visit www.biotek.com...

  • Page 4: Intended Use Statement

    However, the operator should be aware of certain situations that could result in serious injury; these may vary depending on the instrument model. See Hazards and Precautions. BioTek Instruments, Inc.
  • Page 5 Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid. Contact BioTek TAC for assistance. Warning! Unspecified Use. Failure to operate the equipment according to the guidelines and safeguards specified in this manual could result in a hazardous condition.
  • Page 6 Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The instrument should be serviced by BioTek-authorized service personnel. Only qualified technical personnel should perform service procedures on internal components.
  • Page 7 Precautions | vii Caution: Shipping Hardware.The shipping hardware must be removed before operating the instrument and reinstalled before repackaging the instrument for shipment. Caution: Disposal. Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances.
  • Page 8 EN 61010-1. "Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements." EN 61010-2-081. “Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.” EN 61010-2-010. “Particular requirements for laboratory equipment for the heating of materials.“ BioTek Instruments, Inc.

  • Page 9: Electromagnetic Interference And Susceptibility

    Electromagnetic Interference and Susceptibility | ix Directive 2012/19/EU: Waste Electrical and Electronic Equipment Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances. Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use) •...

  • Page 10: User Safety

    Part 1: General requirements.” • Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.” • EN 61010 Standards, see CE Mark starting on page viii. BioTek Instruments, Inc.

  • Page 11: Safety Symbols

    Safety Symbols | xi Safety Symbols Some of the following symbols may appear on the instrument or accessories: Warning, risk of crushing or pinching Alternating current Attention, risque d'écrasement et pincement Courant alternatif Warnen, Gefahr des Zerquetschens und Wechselstrom Klemmen Corriente alterna Precaución, riesgo del machacamiento y Corrente alternata...
  • Page 12 Safety Symbols BioTek Instruments, Inc.

  • Page 13: Installation

    Installation...

  • Page 14: Package Contents

    GEN5RC 1. Unpack and Inspect the Reader Save all packaging materials. If you need to ship the reader to BioTek for repair or replacement, you must use the BioTek-supplied materials. Using other forms of commercially available packaging, or failing to follow the repackaging instructions, may void your warranty.

  • Page 15: Remove The Shipping Hardware

    Remove the Shipping Hardware | 3 2. Remove the Shipping Hardware Remove and store all shipping hardware before you turn on the reader. 1. Carefully turn the reader upside down on a level surface. 2. Remove the six black screws holding the cover to the base, then lift the base off the cover and set it right side up on a level surface.

  • Page 16: Verify The Filter Wheel Contents

    (discussed later). All five locations in the wheel must contain either a filter or a plug. Before continuing, obtain a clean, lint-free cloth. BioTek Instruments, Inc.
  • Page 17 Verify the Filter Wheel Contents | 5 For models with incubation capability: You must first remove the incubation housing to access the filter wheel. 1. With the top case removed from the reader (see Remove the Shipping Hardware on page 3 for directions), remove the four thumbscrews from the optic arm, and lift the optic arm cover off (if equipped).
  • Page 18 Compare the filters with your model’s default filters or with the purchase order if different filters were ordered. Contact BioTek Customer Care if you did not receive the expected filters/plugs. Each filter is labeled with a wavelength and an arrow showing the light direction.

  • Page 19: Select An Appropriate Location

    Select an Appropriate Location | 7 3. When you are finished examining the filters, replace the filters in the filter wheel, being careful to insert them in the correct direction (indicated by the arrow printed on the side of each filter). 4.

  • Page 20: Install The Power Supply

    6. (Optional) Install Gen5 on the Host Computer There is a certain sequence of events that must be followed to ensure that the software is properly installed and configured. Please follow the instructions provided in Gen5 Getting Started Guide to install the software. BioTek Instruments, Inc.

  • Page 21: Optional) Install The Usb Driver

    (Optional) Install the USB Driver | 17 7. (Optional) Install the USB Driver Refer to the instructions that shipped with the Gen5 software to install the necessary drivers. The driver must be installed on the computer before you connect the instrument.
  • Page 22 11. Verify the Reader's Filter Table Before using the 800 TS, verify that the filter table reflects the filters installed in the filter wheel. Important! The reader’s filter table must exactly match the contents of the installed filter wheel. BioTek Instruments, Inc.

  • Page 23: Optional) Establish Communication

    (Optional) Establish Communication | 19 1. From the Main Menu, tap Instrument, the Config tab, and then Filter Table. Filter wheel locations 1 through 5 are shown, with each location’s filter value (in nm) or “plug” for a blank filter. 2.
  • Page 24 Is the touchscreen at the Main Menu? If you remain unable to get Gen5 and the reader to communicate with each other, contact BioTek’s Technical Assistance Center. 13. Run a System Test Running a system test will confirm that the reader is functioning properly, or will provide an error code if a problem is detected.
  • Page 25 Run a System Test | 21 b. On the Incubate tab of the Quick Menu screen, turn on the Temperature Control and enter a setpoint of at least 37°C, then tap Home to return to the Main Menu. c. Wait for the temperature display to reach the defined setpoint before continuing. 2.
  • Page 26 • The Gen5 software stores system test information in its database; you can retrieve it at any time. • You can save the system test report as a text file: click Save As in the System Test Results dialog. BioTek Instruments, Inc.

  • Page 27: Repackaging And Shipping Instructions

    • Shaft shipping bracket screws (PN 19337) • If applicable, top incubation shipping bracket (PN 1562072) If you need to ship the 800 TS to BioTek for service or repair, be sure to use the BioTek-supplied packaging materials. Other forms of commercially available packaging are not recommended and can void the warranty.
  • Page 28 24 | Repackaging and Shipping Instructions BioTek Instruments, Inc.

  • Page 29: Getting Started

    Getting Started...
  • Page 30 The filter wheel contains up to five filters and is user accessible. For models with fewer than five filters, removable plugs are installed in the empty wheel locations. The 800TS and 800TSI models use a tungsten bulb as their light source. The other models, 800TSUV, 800TSUVI, and 800TSNB, use a halogen bulb.

  • Page 31: External Components

    | 27 External Components 1 Microplate carrier 2 Touchscreen 3 Power switch 4 USB flash drive port for USB flash drive Note that the photo does not show the door. 800 TS...
  • Page 32 28 | External Components Rear ports on a reader without incubation capability 1 Power port 2 USB port 3 DO NOT USE! For BioTek Service only 4 USB port for printer Rear ports on a reader with incubation capability 1 Power port...

  • Page 33: Operate The Reader Using The Touchscreen

    Operate the Reader Using the Touchscreen| 29 Operate the Reader Using the Touchscreen General Information Do not use a sharp stylus or pencil on the touchscreen. Doing so can damage the touchscreen's surface. You can use a stylus designed for capacitive touchscreens. When you turn on the 800 TS, the touchscreen turns on automatically and opens to the Main Menu after the start-up system test and bulb warm-up.
  • Page 34 30 | Operate the Reader Using the Touchscreen 800TSI and 800TSUVI models: When the incubator is turned on, the reader’s internal temperature is displayed at the top of the screen, as shown above. When the incubator is off, dashes are displayed.
  • Page 35 Operate the Reader Using the Touchscreen| 31 1. From the Main Menu, tap Instrument, the Config tab, then the Time button. 2. Tap the hour value, and use the keypad to enter the correct time for both the hour and minutes, then click Note: You can change just the minutes value: Tap the minutes value and enter the correct time.
  • Page 36 If you want to send results to a printer connected to the reader: 1. From the Main Menu, tap Instrument, then tap the Output tab. 2. Toggle the Printer button to Enabled, and select the data to be included on the printout (you can select more than one): BioTek Instruments, Inc.
  • Page 37 Operate the Reader Using the Touchscreen| 33 • OD: The raw measurement value for each well. • Delta OD: Applicable only when a secondary wavelength is selected. This is the calculated value for each well of the primary wavelength measurement minus secondary wavelength measurement.
  • Page 38 Set the shake’s duration using the keypad and intensity by toggling through the options. 9. Tap Save. The protocol now appears in the protocol list. *Not all instruments support 384-well microplates. BioTek Instruments, Inc.
  • Page 39 Operate the Reader Using the Touchscreen| 35 Start a Protocol For instruments equipped with incubation capability: To perform incubation during measurement, either turn on incubation from the Incubate tab (Quick Menu) or tap the temperature to the left of the Run Protocol (in a defined protocol). The onboard storage for the touchscreen can accommodate data for up to 12 microplate reads.

  • Page 40: Operate The Reader Using Gen5 Software

    BioTek Customer Care. BioTek Gen5 software supports all 800 TS reader models. Use Gen5 to control the reader; perform data reduction and analysis on the measurement values; print or export results;...

  • Page 41: Maintenance

    Maintenance...

  • Page 42: Maintenance Overview

    Do not allow water or other cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Do not soak the touchscreen! This will cause damage. Moisten a clean cloth with deionized or distilled water and wipe the touchscreen.

  • Page 43: Clean Exposed Surfaces

    Clean Exposed Surfaces | 39 Warning! Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosol contamination. Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible.
  • Page 44 Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazard(s) they handle.
  • Page 45 Decontamination | 41 Wear prophylactic gloves when handling contaminated instruments. Gloved hands should be considered contaminated at all times; keep gloved hands away from eyes, mouth, and nose. Eating and drinking while decontaminating instruments is not advised. Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosol contamination.

  • Page 46: Filter Storage And Handling

    This will ensure the longest life for the filters. When handling the filters, keep the surfaces clean from fingerprints and debris by simply wiping with a lens tissue or other lint-free cloth. BioTek Instruments, Inc.
  • Page 47 Filter Storage and Handling | 43 When changing or replacing filters, it is critical that the filters be placed in the filter wheel in the correct orientation, with the light-direction arrow pointing downward. Also, the reader or Gen5 software filter table must exactly match the contents of the filter wheel.
  • Page 48 44 | Filter Storage and Handling BioTek Instruments, Inc.
  • Page 49 Instrument Testing...

  • Page 50: System Test

    Main Menu appears on the touchscreen. If any test results do not meet the internally coded Failure Mode Effects Analysis (FMEA) criteria established by BioTek, the reader beeps repeatedly and an error message appears on the touchscreen. If this occurs, tap on the touchscreen to stop the beeping.
  • Page 51 Absorbance Test Plates | 47 If you need to change the wavelength values, click Wavelength List. Add, change, or delete the values as needed and click OK. 7. Review all of the values that you entered. When finished, click to save the information.

  • Page 52: Liquid Tests

    • 100-mL volumetric flask 1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask. 2. Add 95 mL of DI water; cap and shake well. The solution should measure approximately 2.000 OD when using 200 µL in a flat-bottom microwell.
  • Page 53 Liquid Tests | 49 Procedure Be sure to use a new microplate. Debris, fingerprints, or scratches may cause variations in readings. 1. Using freshly prepared stock solution (Solution A or B), prepare a 1:2 dilution using deionized water (one part stock, one part deionized water; the resulting solution is a 1:2 dilution).
  • Page 54 ±1.0% ±0.010 OD, then the expected range for the Mean of the well in its Turnaround (H12) position is 1.873 to 1.931 OD. 1.902 x 0.010 + 0.010 = 0.029; 1.902 - 0.029 = 1.873; 1.902 + 0.029 = 1.931. BioTek Instrument, Inc.

  • Page 55: Specifications

    Specifications...

  • Page 56: General Specifications

    40W maximum, non-incubated models; 150W maximum, incubated Power Consumption: models Temperature control for 800TSI model: 6°C over ambient to 50°C. Incubation: Temperature control for 800TSUVI model: 8°C over ambient to 50°C Temperature stability and uniformity is ± 0.5°C across the plate @ 37°C.

  • Page 57: Absorbance Specifications

    General Specifications | 53 Absorbance Specifications Standard Models (800TS/800TSI) Wavelength Range: 400 nm to 750 nm Absorbance Measurement Range Normal Read Mode: 0.000 to 4.000 OD Rapid Read Mode: 0.000 to 4.000 OD Sweep Read Mode: 0.000 to 3.000 OD Absorbance Resolution 0.001 OD when operated in standalone mode...
  • Page 58 ±1.0% ±0.020 OD from 0.000 to 1.000 OD @ 405 nm Linearity (340 nm to 399 nm) Normal Read Mode: ±2.5% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm Rapid Read Mode: ±2.5% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm BioTek Instruments, Inc.
  • Page 59 General Specifications | 55 Linearity (400 nm to 750 nm) Normal Read Mode: ±1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm ±3.0% ± 0.010 OD from 2.000 OD to 3.000 OD @ 450 nm Rapid Read Mode: ±2.0% ±...
  • Page 60 From carrier start to carrier stop: 96-Well, Single Wavelength, Normal Read Mode: 38 seconds 96-Well, Single Wavelength, Rapid Read Mode: 26 seconds 384-Well, Dual Wavelength, Normal Read Mode: 3 minutes, 40 seconds 384-Well, Dual Wavelength, Rapid Read Mode: 2 minutes BioTek Instruments, Inc.

This manual is also suitable for:

800 ts series800ts800tsnb800tstsuv800tsuvi

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