Synergy Ht Multi-Mode Microplate Reader - Biotek Synergy HT Operator's Manual

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Chapter 1: Introduction
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Synergy HT Multi-Mode Microplate Reader

The Synergy HT is a single-channel absorbance, fluorescence, and luminescence
microplate reader. It is computer-controlled using BioTek's Gen5 software for all
operations including data reduction and analysis.
version 2.24 or greater
accessible and compatible with BioTek's BioStack Microplate Stacker.
When making fluorescence
halogen lamp with interference filters for wavelength specificity in conjunction with a
photomultiplier (PMT) tube detector. The Synergy HT has both top and bottom probes for
fluorescence measurements. The top probe can be adjusted vertically for the correct
reading height, via Gen5's Read Height reading parameter (see
Started ).
Luminescence
position in the Emission filter wheel. A filter can also be left in place if light filtering is
necessary.
Absorbance measurements are made by switching to a xenon flash lamp and a
monochromator for wavelength selection. The use of a xenon flash lamp allows for both
UV and visible light absorbance measurements. The monochromator provides wavelength
selection from 200 to 999 nm in 1-nm increments.
The Synergy HT has a 4-Zone
50°C (122°F) that ensures superior temperature uniformity necessary for kinetic assays.
Internal plate shaking is also supported.
All Synergy HT models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry, as well as the BioTek Take3 and Take3
Trio Micro-Volume Plates. Absorbance mode reads plates up to 0.8" (20.3 mm) in height;
fluorescence mode reads plates up to 1.25" (31.75 mm). Polymerase Chain Reaction (PCR)
tubes up to 1.25" (31.75 mm) are also readable with the use of existing adapter plates.
The Time-Resolved (TR) option allows
using the xenon flash light source in conjunction with the PMT measurement detector. A
special cartridge installed in the Excitation filter wheel location is required.
Models with injectors
microplates with standard 128 x 86 mm geometry. An
pumps fluid from the supply bottles to the two injectors located inside the instrument.
Both injectors are positioned directly above the bottom probe, and fluid is injected into one
well at a time.
is required for use with Gen5. The Synergy HT is robot
determinations, the Synergy HT uses a tungsten quartz
is measured by the low-noise PMT detector through an empty filter
temperature control
support dual-reagent dispensing to 6-, 12-, 24-, 48-, and 96-well
Note: Synergy HT basecode software
from 4°C (39.2°F) over ambient to
time-resolved fluorescence
external dispense module
Chapter 3, Getting
measurements by
BioTek Instruments, Inc.