Biotek Synergy HT Operator's Manual page 117

# Step Type
1 Dispense
2 Plate Out,In
3 Dispense
4 Plate Out,In
5 Dispense
6 Plate Out,In
7 Shake
8 Read
9 Read
Customize the Plate Layout (Optional)
The results analysis worksheet at the end of this chapter requires the calculation of
the Standard Deviation, Mean, and % CV of the ODs read for each dispense
volume in each plate (six sets of calculations). By identifying the wells by their
dispense volumes in the Plate Layout, Gen5 will calculate these values for you.
Synergy HT Operator's Manual
Procedure Steps
Details
Dispenser <select 1 or 2, depending on the protocol>
Dispense to wells A1..H4
Dispense 80 µL at rate 275 µL/sec
Suggested comment: Weigh the plate (80 uL test). RECORD the
weight, TARE the balance. Place the plate back on the carrier. Click
OK to continue.
Dispenser <select 1 or 2, depending on the protocol>
Dispense to wells A5..H8
Tip prime before this dispense step, 20 µL
Dispense 20 µL at rate 250 µL/sec
Suggested comment: Weigh the plate (20 uL test). RECORD the
weight, TARE the balance. Place the plate back on the carrier. Click
OK to continue.
Dispenser <select 1 or 2, depending on the protocol>
Dispense to wells A9..H12
Tip prime before this dispense step, 5 µL
Dispense 5 µL at rate 225 µL/sec
Suggested comment: Weigh the plate (5 uL test). RECORD the
weight. PIPETTE 150 ul/well of DI water into all 12 columns. Place
the plate back on the carrier. Click OK to perform the Read steps.
Medium intensity for 15 seconds
Step label: "80 uL Read_Disp 1" (or _Disp 2)
Wells: A1..H4
Detection Method: Absorbance
Read Type: Endpoint
Read Speed: Normal
Two Wavelengths: 405 and 750 nm
Step label: "20 and 5 uL Read_Disp 1" (or _Disp 2)
Wells: A5..H12
Detection Method: Absorbance
Read Type: Endpoint
Read Speed: Normal
Two Wavelengths: 630 and 750 nm
Dispense Module Tests |
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