Biotek Epoch 2 Instructions For Use Manual
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Biotek Epoch 2 Instructions For Use Manual

Microplate spectrophotometer
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Epoch 2
Microplate Spectrophotometer
INSTRUCTIONS FOR USE
BioTek Instruments, Inc.
1771011
Revision
A

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Questions and answers

Tabata
February 3, 2025

How to deselect wells or resign them?

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1 comments:
Mr. Anderson
February 10, 2025

To deselect or resign wells on the Biotek Epoch 2, follow these steps:

1. When running a protocol, select the wells to be read. If not reading the full plate, manually choose specific wells.
2. If the Take3 Blank Masking screen appears and highlights invalid blank wells in red, tap a well to mask (exclude) it from blank average and %CV calculations.
3. Each masked blank well will be flagged with an asterisk in the printed and exported results.
4. Ensure at least one blank well remains unmasked; otherwise, the CV Status will be INVALID.

This process allows users to exclude specific wells from analysis.

This answer is automatically generated

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Summary of Contents for Biotek Epoch 2

  • Page 1 Epoch 2 Microplate Spectrophotometer INSTRUCTIONS FOR USE BioTek Instruments, Inc. 1771011 Revision...
  • Page 2 Preface Page 2 of 42 Contents Installation ......................12 Getting Started ..................... 17 Maintenance ......................33 Qualification ......................39 Specifications......................43 Epoch 2 Instructions for Use 1771011 Revision BioTek Instruments, Inc.
  • Page 3 Inc. Trademarks ® BioTek is a registered trademark, and Epoch™, Take3™, BioStack™, and Gen5™ are trademarks of BioTek Instruments, Inc. BioCell™ is a trademark of BioTek Instruments and is patented under U.S. patent number 5,963,318. ® ® ® Microsoft Windows...
  • Page 4 Page 4 of 42 Contact Information Global Service and Support Instrument service and repair is available worldwide www.biotek.com/service_support U.S. Headquarters BioTek® Instruments, Inc. 100 Tigan Street Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA www.biotek.com Customer Service/Sales: 802-655-4740 or customercare@biotek.com...
  • Page 5 Warning! Service. Only qualified technical personnel should perform service procedures on internal components. Warning! Accessories. Only accessories that meet the manufacturer's specifications shall be used with the instrument. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 6 Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The instrument should be serviced by BioTek-authorized service personnel. Only qualified technical personnel should perform service procedures on internal components.
  • Page 7 (e.g., unshielded intentional RF sources), because these may interfere with the proper operation. Caution: Touchscreen. Do not use sharp implements to operate the touchscreen. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 8 61010-2-010. "Particular requirements for laboratory equipment for the heating of materials." Directive 2012/19/EU: Waste Electrical and Electronic Equipment Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local ordinances. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 9 • Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.” • 61010 Standards, see Mark starting on page 8. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 10 Off (Supply) Caution (refer to accompanying documents) Warning, risk of electric shock Warning, risk of crushing or pinching Warning, potential biohazards Warning, hot surface Laser radiation: Do not stare into beam 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 11 Laser energy: Exposure near aperture may cause burns. Do not stare directly at the laser during operation. In vitro diagnostic medical device Separate collection for electrical and electronic equipment Consult instructions for use 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 12 • EPOCH2TSC • • Package Contents Item Notes A specific model of an Epoch 2 instrument per sales order All required accessories to power the instrument Interface cables, if required User manual and/or instructions Optional accessories per sales order, unless shipped separately 1771011 BioTek Instruments, Inc.
  • Page 13 Installation Page 13 of 42 Unpack the Box 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 14 (Gen5 control only) Prepare the Host Computer ❖ Follow instructions in the Gen5 Getting Started Guide to install the software. (Gen5 control only) Connect the Host Computer and Reader 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 15 Installation Page 15 of 42 Install the Power Supply Install the Cuvette Holder Models with a cuvette port 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 16 1. Start Gen5. If prompted to add a reader, click Yes. Otherwise, select System > Instrument Configuration > Add Reader. 2. Select Epoch 2 and click OK. The reader name and serial number appear. Click on the reader name and then click Test Communications. The message, “The reader is...
  • Page 17 2. microplate carrier access door 3. cuvette holder (if equipped) 4. power on/off 5. carrier in/out, status port for printer 7. power inlet port for host computer (if used) 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 18 Page 18 of 42 Non-Touchscreen Models 1. microplate carrier access door 2. cuvette holder (if equipped) 3. power on/off 4. carrier in/out, status 5. power inlet port for host computer 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 19 Take3 plate (if used). To run an existing protocol, tap its name, define the wells to be read if fewer than the full plate, and tap Start. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 20 • Set the Decimal Symbol to a period or comma. • Set the List Separator, used in exported .csv files, to a comma or semicolon. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 21 This file can be opened in Gen5 using the Read from File option. • Prompt: Choose an output format after the plate has been read. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 22 4. Adjust the Frequency, if needed. The cycles per minute updates as the slider moves. The measurement in mm indicates the distance the carrier travels during the shake. 5. Place the plate on the carrier and tap Start to shake the plate. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 23 The gradient represents the temperature separation between the top and bottom heaters. This differential should serve to reduce or eliminate condensation. In the example below, the Set point is 37°C and the Gradient is 1.0: 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 24 200 to 999 nm. Microplates and Take3 only: Read Speed: Select Normal (optimum performance) or Sweep (optimum speed). See Specifications to compare the performance and timing specifications. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 25 If a shake is specified, its Duration may result in a longer interval than defined in the protocol. To test the 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 26 CVs. • To define a kinetic protocol with a shake before the first read only, use Gen5. When finished, tap Save. The protocol name is added to the Main Menu. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 27 The measurement in mm indicates the distance the carrier travels during the shake. When finished, tap Save. The protocol name is added to the Main Menu. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 28 2. Tap the protocol that you want to modify, then tap Edit. 3. Make any desired changes, then tap Save. Delete a Protocol 1. From the Main Menu, tap Protocol. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 29 2. Tap the green button in the upper-right corner of the display. The results are printed and/or saved to the flash drive, depending on the read mode and defined output format. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 30 Getting Started Page 30 of 42 Run a Take3 Session Using the Touchscreen Epoch 2 is preprogrammed with seven Take3 protocols, available for use after a Take3 plate has been defined and aligned on the reader. The protocols measure at 260, 280, and 320 and cannot be edited.
  • Page 31 Run Protocol screen, select options as applicable: • Concentration units: Select the unit of measure for the export file. • Secondary Ratio WL (230 nm): Available for nucleic acid protocols. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 32 • Each masked blank well will be flagged with an asterisk in the printed and exported results. 5. When the read is finished, the results are displayed and ready for output according to the settings under Instrument > Output. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 33 5. If detergent was used, wipe all surfaces with a cloth moistened, not soaked, with water. 6. Use a clean, dry, lint-free cloth to dry all wet surfaces. If liquid is spilled inside the reader, contact BioTek Technical Assistance Center. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 34 3. Wipe the touchscreen gently with the moist towel. 4. If detergent was used, wipe the touchscreen with a towel moistened, not soaked, with water. 5. Dry the screen gently using another towel. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 35 Do not use a stronger cleaning solution than recommended. Materials • Sodium hypochlorite (NaClO) or 70% isopropyl alcohol • Deionized or distilled water • 125-mL beakers • Clean, lint-free cotton cloths • Protective gloves • Lab coat 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 36 Do not soak the cloth. 8. Use a clean, dry lint-free cloth to dry all wet surfaces. 9. Discard the used gloves and cloths, using a biohazard trash bag and an approved biohazard container. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 37 Instrument Testing System Test Each time the Epoch 2 is turned on, it automatically performs a series of tests on the reader’s motors, lamp, and various subsystems. This test can take a few minutes to complete. If all tests pass, the microplate carrier is ejected and the green on the power switch remains on.
  • Page 38 6. Place the Absorbance Test Plate on the microplate carrier, with well in the proper location. 7. Click to run the test. 8. When the test is complete, the results report appears. Scroll through the report; every result should show “PASS.” 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 39 Specifications Page 39 of 42 Specifications General Specifications Microplates Epoch 2 accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry and the Take3 Micro-Volume Plate. If using Gen5, the Take3 Trio Micro-Volume plate BioCell are also supported.
  • Page 40 0.000 to 1.500 OD: ±2.0% ±0.010 normal read speed 1.500 to 2.000 OD: ±5.0% ±0.010 delay after plate movement = 100 96- and 384-well plate 0.000 to 1.000 OD: ±1.0% ±0.010 sweep read speed 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 41 Cuvette Absorbance performance of measurements made via cuvette shall satisfy the requirements given above for accuracy, linearity. and repeatability in a 96 well-plate, normal read speed, 100 delay after movement. 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...
  • Page 42 [sec] MAX [msec] Normal Mode Normal Mode, Min Delay Time Sweep 0 (at end of row) 384 well Normal Mode Normal Mode, Min Delay Time Sweep 0 (at end of row) 1771011 BioTek Instruments, Inc. Epoch 2 Instructions for Use Revision...

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