Biotek Synergy HT Operator's Manual page 174

148
Appendix C: Error Codes
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Code
Light beam saturated (too much light)
0508
Air measurement channel reading reached 65535.
This error indicates that during background tests or prior to a read, the PMT reached saturation.
When at rest, the PMT is charged to 650 volts to maintain stability for the next read. The Synergy
is constantly testing the PMT when idle. If light reaches the PMT, this error can occur.
Probable Causes:
• Light leakage or bright light in the read chamber.
• The EX filter wheel cartridge was removed prior to a Time-Resolve read, and ambient or
light from the lamp reached the PMT.
• Fluorescence analog PCB has intermittently failed.
• EM and EX filter wheel overlap. If a hole in the EM wheel is required, try positions 1 or 4.
(Basecode v2.14 or higher has greatly reduced the possibility of filter wheel overlap.)
• The filters were not placed in the filter wheel in order of ascending wavelength from top to
bottom.
Reference channel light beam saturated (too much light)
0510
Air reading reached 65535.
This error can indicate one of the following scenarios:
• (1) During an absorbance filter calibration or wavelength scan, the measurement channel
was saturated when storing to memory.
• (2) During a spectral scan read, the measurement channel was saturated for one of the
wavelengths.
Probable Causes:
Scenario 1 (Filter calibration):
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB has intermittently failed.
• Missed flashes or an erratic flash lamp.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.
Scenario 2 (Spectral scan read):
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB intermittently failed.
• Missed flashes or an erratic flash lamp.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.
Description and Probable Causes:
BioTek Instruments, Inc.