Fluorescence Tests - Biotek Synergy HT Operator's Manual

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Chapter 4: Instrument Qualification
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Fluorescence Tests

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The Corners Test
properly aligned in relation to the optical probe(s). (Because the Synergy HT's
fluorescence optics are different from the absorbance optics, the Corners Test is
also required.) We recommend running the test for both the top and bottom probes
(if equipped).
•
The Sensitivity Test
test the fluorescence reading capability of the reader. The ability to detect specific
compounds at low concentrations ensures that the filters, optical path, and PMT
are all in working order. This test verifies that the difference between the means of
wells with known lower limits of concentration of the substance under
investigation is statistically distinguishable from the mean of wells with pure
diluent.
•
The Linearity Test
proportionally with changes in concentration. Proving that the system is linear
allows the Sensitivity Test to be run on two points instead of using serial dilutions.
Since it is somewhat difficult to achieve high pipetting accuracy when
conducting linear dilutions, an
considered adequate.
uses fluorescent compounds to verify that the plate carrier is
uses fluorescent compounds of varying concentrations to
verifies that the system is linear; that is, signal changes
Important! The tests presented in this section require specific
microplates, solutions, and EX/EM filters. Your laboratory
may require a deviation from some of these tests. For example,
you may wish to use a different fluorescing solution and/or
microplate.
If deviation from the tests as presented in this section is
required, the following steps should be taken the first time
each test is run (e.g., during the
1. Perform the tests exactly as described on the following
pages.
2. Rerun the tests using your particular solutions, filters,
microplates, etc. If results are comparable, then the results
from these tests will be your baseline for future tests.
3. Be sure to document your new test procedure(s), and save
all test results.
R Square value of at least 0.99 is
Initial OQ ):
BioTek Instruments, Inc.