Biotek Synergy HT Operator's Manual page 171

Code
Light beam saturated (too much light). Air reading reached 65535 or PMT Relative
0500
Fluorescing Units (RFU) reached FFFF (99999).
This error can indicate one of the following scenarios:
• (1) During an absorbance filter calibration or wavelength scan, the reference channel is
saturated when storing to memory.
• (2) During a fluorescence-dispensing read, after dispensing, the read times-out due to the
sample saturating the PMT.
• (3) During a spectral scan read, the reference channel reached saturation at one or more of
the selected wavelengths.
• (4) During fluorescence autocalibration, the light level did not reduce to more than half the
saturation light level when the carrier was moved away from center.
Probable Causes:
Scenario 2 (fluorescence-dispensing read):
• Incorrect chemistry was dispensed into the well.
Scenarios 1 and 3 (spectral scan):
• Monochromator has a defect in the mirror gradients.
• Absorbance analog PCB intermittently failed.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.
Scenario 4 (fluorescence autocalibration):
• The surface of the autocalibration jig is too reflective. Return the jig to BioTek for a
replacement jig.
• The carrier did not move far enough to block the light beam. Troubleshoot the carrier
movement.
0501 Light beam saturated (light). Air measurement channel reading reached 65535 or
PMT Relative Fluorescing Units (RFU) reached FFFF (99999).
This error can indicate one of the following scenarios:
• (1) Prior to a fluorescence read or during a system test, the PMT is tested for operation; the
gain is set at 153. Is the PMT operating properly?
• (2) The PMT is constantly being checked for an overload condition.
• (3) During a spectral scan read, the measurement channel reached saturation at one or
more of the selected wavelengths.
• (4) Filter 1 in absorbance mode has saturated the measurement channel.
Probable Causes:
Scenarios 1 and 2 (fluorescence or system test):
• The PMT is defective.
• The connector from the PMT base to the analog board is defective. The ground/shield is
not grounding properly.
• The PMT base is defective.
• The PMT analog PCB is defective.
Scenario 3 (Spectral scan read):
• The monochromator mirror/grating is damaged.
• Absorbance analog PCB intermittently failed.
• Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
• Missed flashes or an erratic flash lamp.
• Voltage to the lamp has increased due to failure of the lamp power supply, or the motor
Synergy HT Operator's Manual
Description and Probable Causes:
145
Error Codes |