The context does not provide specific Monthly Maintenance instructions for the BioTek 405 TS Microplate Washer. It only mentions general maintenance guidelines and suggests developing a maintenance schedule based on fluid characteristics and use frequency. Therefore, the Monthly Maintenance instructions are not found in the provided information.

This answer is automatically generated

Summary of Contents for Biotek 405 TS

Page 1 Microplate Washer ™ Getting Started Guide...
Page 3 Review the best practices for optimal performance. Review the Safety Information page 85 Copy the 405 TS Operator's Manual your computer to make it easy to find reference information in the future. The complete operator's manual is delivered on the USB flash drive shipped with the instrument.
Page 4: Table Of Contents
2 | 405 TS Getting Started Guide Contents Quick Start Notices Global Service and Support BioTek's Customer Resource Center Install the 405 TS Unpack and Inspect the Instrument Remove the Shipping Hardware Setting Up the 405 TS Install Software/Connect to Computer...
Page 5 Washer Problems Specifications and Part Numbers Physical Specifications Performance Specifications Package Contents Optional Accessories Index ....................... Chemical Compatibility 405 TS Customer Training Guide BioTek Instruments, Inc.
Page 6: Notices
BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.
Page 7: Global Service And Support
Global Service and Support| 5 Global Service and Support BioTek instrument service and repair is available worldwide at several of BioTek’s International Service Centers and in the field at your location. Contact the office nearest you to arrange service or to get answers to your technical questions, call the Technical Assistance Center (TAC) at 802-655-4740 in the US.
Page 8 6 | 405 TS Getting Started Guide BioTek South Korea BioTek Switzerland 3F, Gyungnam building, 830-48 Zentrum FanhÖfli 8 Yeoksam-dong, Gangnam-gu 6014 Luzern Seoul, South Korea (135-936) Switzerland Phone: +82 (0) 2-562-4740 Phone: +41 (41) 2504060 Fax: +82 (0) 2-562-4750 Fax: +41 (41) 2505064 Email: korea@biotek.com...
Page 9: Biotek's Customer Resource Center
When operated in a safe environment, according to the instructions in this document, there are no known hazards associated with the 405 TS. However, the operator should be aware of certain situations that could result in serious injury; these vary depending on the instrument type. See Hazards and Precautions.
Page 10 Repackaging and Shipping If you need to ship the instrument to BioTek for service or repair, contact BioTek for a Return Materials Authorization (RMA) number and use the original packing materials. Other forms of commercially available packaging are not recommended and can void the warranty.
Page 11: Install The 405 Ts
Install the 405 TS Follow the instructions in this section to install your instrument. Unpack and Inspect the Instrument Remove the Shipping Hardware Setting Up the 405 TS Install Software/Connect to Computer Connect to Power Define Instrument Settings Verify Performance...
Page 12 Failure to remove and reinstall the shipping bracket may void your warranty. Keep in mind that you must reinstall the shipping hardware and use the original shipping material if it is necessary to return the instrument to BioTek for service or repair. 405™ TS Microplate Washer...
Page 13 For direct drain, an intermediate collection bottle is attached to the washer using the same studs as the shipping bracket. See the Direct Drain Kit instructions. 405 TS with Verify™ Technology "Q" Models "Q" Models have three shipping brackets:...
Page 14 12 | 405 TS Getting Started Guide 1. Use the 9/64” hex wrench to unscrew the cap screws and remove the bracket on the side of the wash manifold that holds the Verify sensor in place. 2. Remove the large bracket in front holding the wash manifold: Remove the three screws.
Page 15 7. Store the brackets: mount them on the back panel on the studs provided. Beginning with the longest (3rd) bracket, layer the brackets on top of each other, and use the longest screws to secure them in place. BioTek Instruments, Inc.
Page 16 50% relative humidity at 40°C. Connect the Vacuum Pump, Tubes, and Bottles For optimal operation of the 405 TS, all tubing, cables, and fittings for the fluid supply and waste systems must be properly connected. This image illustrates the rear panel of the instrument and the locations of the ports and connections for the fluid supply and waste systems.
Page 17 Accessory Outlet on the back of the instrument. This allows the 405 TS to regulate the pump, turning it on and off as specified by the protocol. When using a standard pump (rather than the high flow pump), set the instrument’s...
Page 18 This will help optimize performance. 8. Make sure the waste bottle’s caps are well sealed. BioTek strongly recommends installing the vacuum line filter to protect your vacuum pump. Install the Vacuum Line Filter The optional vacuum line filter (PN 48294) can be installed halfway between the last waste bottle (overflow bottle) and the vacuum pump.
Page 19 Setting Up the 405 TS| 17 Install the Fluid Supply System Prepare the supply bottles and tubing: 1. Remove the Quick Release Connector from its bag inside the supply bottle and connect it to the raw supply tubing provided. You may need to cut the tubing to the desired length.
Page 20 18 | 405 TS Getting Started Guide Make sure you hear the connector click! Attach the labels provided with the washer to identify the buffer bottles. Note: To avoid spilling fluid when refilling bottles or changing reagents, first release the Quick Connector from the bottle cap, use a paper towel to sop up the few drops in the cap.
Page 21 Install the Microplate Carrier Make sure the serial number on the underside of the plate carrier matches the washer's serial number. If the numbers do not match, call BioTek TAC immediately. 1. Line up the tab on the underside of the carrier with the slot on the carrier transport block.
Page 22 Install Software/Connect to Computer If you purchased BioTek's Liquid Handling Control™ (LHC) Software to control the 405 TS using your personal computer (PC), please refer to the LHC Installation Guide for complete installation and setup instructions. 405™ TS Microplate Washer...
Page 23: Connect To Power| 21
They cannot be used simultaneously. You can use USB to connect the 405 TS to the computer or the RS232 serial port to connect to a BioStack or similar robotic device. But you cannot use both ports simultaneously, i.e.
Page 24: Define Instrument Settings
Define Instrument Settings LHC Users Only When using the LHC to control the 405 TS, an important first step is defining your instrument’s settings. After installing the LHC, you can use the desktop icon or the Windows Start button to launch the LHC: >...
Page 25 Remember to update the time in accordance with annual time shifts, like Daylight Savings Time, if applicable. Define Startup Preferences (LHC users only) You can save enormous time creating protocols by following these steps to define a template and use it at startup. New Protocol BioTek Instruments, Inc.
Page 26 24 | 405 TS Getting Started Guide Create a protocol template Click the button or select File>New 2. Click . Select the 405 TS and define its Name Port Settings : select the Plate Type , fill in the text fields, and add any Highly Recommended steps that you want all new protocols to include.
Page 27: Verify Performance
Verify Performance Before using the 405 TS for the first time, verify that it is operating properly. When using the LHC, make sure the 405 TS is connected to the PC and both are powered up. When running standalone, turn on the 405 TS.
Page 28 4. When finished, close the program. Run the Verify Test ("Q" Models Only) If your 405 TS is equipped with BioTek's Verify™ Technology, run the test: Important! Before running the test: Make sure the 405 TS is primed and ready to run: Fully prime the tubing/system, e.g.
Page 29 Do not discard the . Reuse it for as long as possible. Verify Test Plate 405 Touch: BioTek recommends exporting the initial test results and saving them for future reference. Contact BioTek TAC if any of the tests fail. BioTek Instruments, Inc.
Page 30 28 | 405 TS Getting Started Guide 405™ TS Microplate Washer...
Page 31: Basic Operation
Basic Operation This section introduces the basics of operating the 405 TS. For more detailed and comprehensive instructions, please refer to the operator's manual on the CD shipped with this guide. Touch Screen Basics Create or Modify a Protocol Verify Performance...
Page 32 30 | 405 TS Getting Started Guide Touch Screen Basics Home screen Fast, Light Touch: a light, quick tap on the screen produces the best results. Use your fingertip or a pointing device like a pen tip. The touch screen does support gestures, like swiping and zooming, that are common to smart phones.
Page 33 Green buttons make the 405 TS perform. Two ways to wash a plate The 405 TS offers two ways to wash a plate: Quick Wash: using the touch screen you can wash a plate by defining a few parameters like fluid volume and number of wash cycles and rely on default parameters for the more advanced options.
Page 34 32 | 405 TS Getting Started Guide Create or Modify a Protocol Select Define at the Home screen To create or edit a protocol: 1. Select at the screen. Define Home Maintenance 2. Press or highlight the protocol and press...
Page 35 Add protocols to the Home screen The 405 TS puts all saved protocols on the Home screen for easy retrieval. Create and save a protocol to add it to the Home screen listing. Remove protocols from the Home screen All saved protocols appear on the Home screen.
Page 36: Verify Performance
Verify Performance Quick>Verify Manifold BioTek's Verify™ Technology quickly detects clogged aspirate or dispense tubes. Important! Before running the test: Make sure the 405 TS is primed and ready to run: Fully prime the tubing/system, e.g. run W-Day Rinse. If necessary, empty waste vessel and tighten waste bottle cap.
Page 37 Handling the Verify Test Plate The Verify Test Plate ships in its own special box. The plate is labeled with the serial number of the 405 TS that has been calibrated for its use. Well A1 is opposite the chamfered or beveled edges.
Page 38 36 | 405 TS Getting Started Guide Important guidelines: Always put the plate on the carrier with well A1 in the back, left corner of the carrier. Clean the plate after each use if you are using wash buffer to run the test.
Page 39: Optimize Performance
See 405 Recommendations for Priming the Washer on the next page Best Practices Fill the supply bottles with sufficient fluid. Never run the 405 TS without the fluid filter installed (on the end of the tubing inside the supply bottle).
Page 40 This is recommended before running a protocol to correct fluid loss due to evaporation. Dead Volume BioTek's recommended prime volumes are based on purity testing and measured dead volumes. Generally, priming with three times the dead volume assures purity.
Page 41 Optimize Performance| 39 Running Predefined Protocols BioTek provides numerous predefined protocols for maintaining the instrument in top condition and for qualifying its performance. Review the Predefined Protocols on page 41 To run a defined protocol: Touch screen 1. Touch a protocol on the...
Page 42 The LHC stores the COM port value in the protocol file. With the 405 TS connected to and communicating with the host computer (i.e. make sure the instrument is turned on and not busy):...
Page 43: Predefined Protocols Listing| 41
Simple prime routine; defined for Buffer valve A only. For LHC 250/300 only. PRIME_ALL_ Consecutively primes each of the Buffer Switching valves BUFFRS beginning with D. Designed for use in the annual instrument verification test of the Buffer Switching module. BioTek Instruments, Inc.
Page 44: Predefined Sample Protocols
42 | 405 TS Getting Started Guide QC (Quality Control) Protocols Manifold-Specific QC_96_DISP_TEST Dispense precision test protocol for 96-tube manifold. QC_96_EVAC_ Evacuation efficiency test protocol for 96-tube manifold. TEST QC_192_DISP_ Dispense precision test protocol for 192-tube manifold. TEST QC_192_EVAC_ Evacuation efficiency test protocol for 192-tube manifold.
Page 45 MAG_plate_ recovery in 96-well round- or flat-bottomed plates and 384-well plates when using BioTek's Flat magnets. Begins by soaking the plate for 1 minute to let the beads settle at the bottom of the wells, then performs a 2 cycle wash with 30 second soaks between cycles.
Page 46 44 | 405 TS Getting Started Guide Default Option Description/Values range values Aspirate Vacuum Filtration: Select standard aspiration (Top) or Vac for filtration. Filtration When applicable, specify duration of vacuum filtration Time: in seconds. 5-999 Travel The rate at which the washer manifold travels down Rate: into the wells.
Page 47 Rate: Valid range is 3-11. The cell wash rates, 1 CW and 2 CW, which use low-flow tubing, are available but not recommended. Cell wash options are designed for gentle washing, while bottom wash is designed for vigorous washing. Wash 25-3000 µL/well dispense Volume: BioTek Instruments, Inc.
Page 48 46 | 405 TS Getting Started Guide Default Option Description/Values range values Positioning: X- and Y- horizontal axes, Z- height (vertical) axis can be adjusted. Repositioning the tubes to harder- to-reach areas of the wells may the improve results. Shake To mix the contents of the plate.
Page 49: Operating With The Biostack
LHC Control: LHC users: connect both the BioStack and the 405 TS to the computer and control them with the LHC. Design protocols that integrate BioStack controls with 405 TS steps. LHC protocols must contain a BioStack loop.
Page 50 See How to define a BioStack protocol on the facing page At the start of the day, power up the BioStack first, and then the 405 TS. BIOSTACK2WR: Lift the BioStack’s gripper before turning it on. Robotics integrators: CAD drawings of the physical dimensions of the 405 TS are available upon request.
Page 51: Use The Biostack| 49
5. Save the protocol. 6. At runtime, define the in the Run Info section of the Number of plates to process run screen: enter a specific number or select the "Entire input stack" for processing. BioTek Instruments, Inc.
Page 52 50 | 405 TS Getting Started Guide Re-stack plates in the BioStack After the plates in the input stack have been processed, they are moved to the output stack. The Restack step moves them back into the input stack in their original order.
Page 53: Cell Wash
The 405 TS is equipped with a low-flow fluid path that provides a "cell wash" alternative for cell- based assays.
Page 54 Advanced Options Vacuum until sufficient fluid has been dispensed. For small dispense volumes, BioTek recommends setting the delay volume to equal your dispense volume. Reposition the dispense tubes to aim fluid at the side of the wells to reduce turbulence and change the aspirate height to increase the amount of residual fluid left in the well to protect the cell layer.
Page 55 Using a standard 96-well Corning Costar plate, best results were achieved with these values: Dispense Step Settings: Z - height = 120 steps X - horizontal position = -45 Y - horizontal position = 0 BioTek Instruments, Inc.
Page 56 54 | 405 TS Getting Started Guide Aspirate Step Settings: Z = 46 steps X = 35 Y = 0 Secondary Aspirate: Z = 80 steps X = -45 Y = 0 About 40 µL/well residual fluid is retained using these values.
Page 57 For cell wash assays, specify at least 10 µL/well. For small dispense volumes or to disable aspiration for the entire dispense duration, set the vacuum-on volume to equal your dispense volume. Refer also to application notes on the BioTek web site for more information ( www.biotek.com BioTek Instruments, Inc.
Page 58: Biomagnetic Separation - Magnetic Bead Assays
Bodily harm [pinching of hands and skin] can result if magnets are not handled correctly. Maintain distance between two or more magnets. Take advantage of the sample magnetic-bead protocols shipped onboard the 405 TS. Copy W-Luminex_Mag_Flat_96, for example, and modify the parameters to suit your assay requirements.
Page 59 Use the Manifold Stop Screw Adjustment Kit on page 62, if necessary. Realign the BioStack with the Magnet Installed on page 57 Change the Magnet Height Offset on page 60 Optimize Magnetic Bead Protocols on the next page BioTek Instruments, Inc.
Page 60 58 | 405 TS Getting Started Guide Optimize Magnetic Bead Protocols Here are some suggestions to consider for optimizing your magnetic bead assays: : Flat-bottom plates are recommended for magnetic bead assays Plate Type because more of their well surface sits closer to the magnet, resulting in increased magnet strength, than with other plate formats.
Page 61 Adjust the X Position to align the aspirate tubes away from the center of the well, near the well walls. Ring Magnets Use this information about the VP magnets to fine-tune wash protocol settings: 96 Ring Magnet 7102216 384 Ring Magnet 7102215 BioTek Instruments, Inc.
Page 62 For the best results, measure the height of the plate you are using with the magnet. BioTek has determined that Nunc flat bottom plates are about 3 mm taller than a similar Corning plate, for example.
Page 63 3. Measure the distance from the bottom of the carrier to the top of the plate in millimeters (mm). 4. Remove the magnet, and measure the plate and carrier combined. 5. Subtract the plate and carrier measurement from the measurement value of the total combined elements. This is the offset value to use. BioTek Instruments, Inc.
Page 64: Manifold Stop Screw Adjustment Kit
Manifold Stop Screw Adjustment Kit For Magnetic Bead Assays and 384-Well PCR Plate Processing To successfully perform these assays, 405 TS Select and HT models may need to adjust the height of the manifold stop screw. The adjustment kit, PN 1170011, is shipped with instruments with a dual manifold.
Page 65 This will lock the nuts in place and allow you to easily remove/replace the screw without affecting its height setting. 6. Verify the height with the jig and repeat steps 3-6 if necessary. BioTek Instruments, Inc.
Page 66: Vacuum Filtration For Filter Plate Assays
Generally, bead assays prefer low pressure and DNA separation assays prefer higher pressure. The best performance in tests at BioTek were seen when the pressure was set to 2.5 inHg. 405™ TS Microplate Washer...
Page 67 Optionally, do the same for the Final vacuum duration. Aspirate 5. Define the Final Aspirate parameters. 7. Fill the checkbox for Shake/Soak specify the shake duration. Experiment with different settings to determine the optimal parameters to meet your goals. BioTek Instruments, Inc.
Page 68 66 | 405 TS Getting Started Guide Important: Be sure to change the Plate Carrier setting to match the installed carrier. Final Aspirate Remember to specify the type of aspiration to perform in a Control the Vacuum Level Vent plugs stored on side of carrier...
Page 69 Vacuum pressure is affected by several factors like relative humidity, barometric pressure, and mechanical tolerances, as well as, filter plate pore size Testing at BioTek confirmed expectations: small pore filter plates and highly viscous fluids require increased vacuum and/or longer aspiration durations to evacuate the wells.
Page 70: Changing The Instrument's Settings
3. Click after changing a Send setting to update the instrument. When using the LHC to change instrument settings, you may need to reboot the 405 TS before controlling it with the touch screen. 405™ TS Microplate Washer...
Page 71: Washer Settings
Custom or non-standard microplates and special adapters for labware may benefit from this setting, too. If a vessel's height has been its only limitation to processing with the 405 TS, i.e. the vessel's geometry permits the manifold tips to successfully address its wells, this setting can be used to simplify protocol development.
Page 72: Autoprime
BioTek recommends keeping the detection systems activated. Exceptions: Deactivate the Waste sensor when using BioTek's Direct Drain Waste System. When vacuum filtration assays need to be performed at very low vacuum levels, e.g.
Page 73 Send settings to the instrument. BioStack Important: The BioStack must be connected to the 405 TS using the dedicated serial cable shipped with the BioStack and turned on. Basecode: this is good communication test. Check the cabling, and make sure the BioStack is turned on if the basecode version is not revealed.
Page 74 It will overwrite the existing file. Watch a training video The 405 TS offers a simple video viewer to show short videos from BioTek. The video player is useful only for BioTek-provided videos with restricted frame speeds and the Windows file format: .wmv.
Page 75 AutoPrime| 73 3. Press Show Video 4. Highlight the .wmv file and press Select BioTek Instruments, Inc.
Page 76 74 | 405 TS Getting Started Guide 405™ TS Microplate Washer...
Page 77: Maintenance
Maintenance Regular maintenance is required to achieve optimal performance with the 405 TS. This section provides guidelines and instructions for basic daily maintenance and the Recommended Maintenance Schedule. Find comprehensive instructions for performing regular maintenance in the operator's manual. Overview...
Page 78 The frequency of conducting these tasks must be based on the risk and performance factors of your assays. Develop a maintenance schedule for your 405 TS based on the characteristics of the fluids used and the activity level. Here are some guidelines for each component: Washer When using fluids prone to dry and harden quickly, the washer’s dispense...
Page 79 Clean washer manifold ü ü Clean aspirate and dispense tubes ü Clean exterior surfaces and mist shield ü ü Clean fluid inlet filter ü ü Clean vacuum filtration system ü ü Clean the Verify™ level sensor Decontaminate BioTek Instruments, Inc.
Page 80 78 | 405 TS Getting Started Guide Frequency Tasks Before Daily Overnight/ Weekly Periodic/ storage/ Multi-Day Monthly shipment ü ü Decontaminate external surfaces ü ü Run W-DECONTAMINATE ü Decontaminate vacuum filtration system Prepare for Storage or Shipment ü Run W-LONG_SHUTDOWN...
Page 81: Daily Maintenance
Overnight/multi-day maintenance involves flushing all solutions out of the instrument, and then periodically rinsing and soaking the tubes to keep them moist. Here are three recommendations for accomplishing the task. Employ the method that best suits your work flow: BioTek Instruments, Inc.
Page 82: Autoclean The Washer
AutoClean the Washer Some models of the 405 TS feature BioTek's Ultrasonic Advantage™ for easy and thorough cleaning of the wash manifold. Instruments with AutoClean capability are easily identified by the stainless steel priming reservoir built into the instrument's base, under the wash manifold.
Page 83 " bottle with detergent or an appropriate solution for your Clean Buffer assay. The 405 TS primes the system with 300 mL/305 mL for CW models and fills the trough with 93 mL for the sonicator. Buffer Switching models: connect it to the specified valve.
Page 84: Removing Protein Residuals And Fungi Growth
Daily Practice with buffer or deionized water: If the 405 TS will be idle between plates for longer than 45 minutes, flush the proteins: 1. Fill a supply bottle with deionized water. Connect the bottle to the washer.
Page 85 2. Remove the fluid filter at the bottom of the tubing. 3. Wash the filter with hot water and a soft- bristled brush, if necessary. 4. Rinse the filter and reinstall it. BioTek Instruments, Inc.
Page 86 84 | 405 TS Getting Started Guide 405™ TS Microplate Washer...
Page 87: Safeguards And Troubleshooting
Safeguards and Troubleshooting This section lists warnings and precautions that must be taken when operating the 405 TS. It also includes guidelines for error recovery and troubleshooting performance problems. Hazards and Precautions CE Mark Electromagnetic Interference and Susceptibility Safety Symbols...
Page 88: Hazards And Precautions
86 | 405 TS Getting Started Guide Hazards and Precautions Hazards The following hazards are provided to help avoid injury: Warning! Power Rating. The instrument’s power supply or power cord must be connected to a power receptacle that provides voltage and current within the specified rating for the system.
Page 89 Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The instrument should be serviced by BioTek authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.
Page 90 40% formaldehyde, and 20% sodium hydroxide. Never use acetic acid, DMSO, or other organic solvents. These chemicals may cause severe damage to the instrument. Contact BioTek for more information and prior to using other questionable chemicals. Caution: Bovine Serum Albumin. Solutions containing proteins, such as bovine serum albumin (BSA), will compromise the instrument’s...
Page 91: Ce Mark
Caution: Waste Sensor Port on 405 TS. (For customers who have purchased the BioStack Microplate Stacker.) Although the waste sensor port on the back of the 405 TS is the same type as the 24-VDC power connector on the back of the BioStack, if an external 24-VDC power supply is plugged into the 405 TS’s port, it will permanently damage...
Page 92: Electromagnetic Interference And Susceptibility
90 | 405 TS Getting Started Guide Verification of compliance was conducted to the limits and methods of the following: EN 61000-4-2, Electrostatic Discharge EN 61000-4-3, Radiated EM Fields EN 61000-4-4, Electrical Fast Transient/Burst EN 61000-4-5, Surge Immunity EN 61000-4-6, Conducted Disturbances from RFI...
Page 93 Part 1: general requirements.” Canadian Standards Association CAN/CSA C22.2 No. 61010-1 “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: general requirements.” EN 61010 Standards, see CE Mark on page 89. BioTek Instruments, Inc.
Page 94: Safety Symbols
92 | 405 TS Getting Started Guide Safety Symbols Some of these symbols appear on the instrument or accessories: Alternating current Both direct and alternating current Courant alternatif Courant continu et courant alternatif Wechselstrom Gleich - und Wechselstrom Corrientealterna Corriente continua y corrientealterna...
Page 95 Elektronikgeräten vitro Recogida selectiva de aparatos eléctricos y electrónicos Raccolta separata delle apparecchiature elettriche ed elettroniche Consult instructions for use Consulter la notice d’emploi Gebrauchsanweisung beachten Consultar las instrucciones de uso Consultare le istruzioni per uso BioTek Instruments, Inc.
Page 96: Troubleshooting
LHC is not responding, or when running a system test doesn't resolve the issue, turn off your computer and 405 TS, check all the cabling, i.e. make sure your USB cable is in good condition and is properly connected to the PC and instrument, and then, power them on.
Page 97: Washer Problems
Washer Problems| 95 can use USB to connect the 405 TS to the computer or the RS232 serial port to connect to a BioStack or similar robotic device. But you cannot use both ports simultaneously, i.e. make sure only one cable is plugged in at a time.
Page 98 96 | 405 TS Getting Started Guide Possible Problem What To Do Cause Uneven Plate carrier Replace plate carrier: contact BioTek TAC for performance. leveling feet assistance. Note: "Q" model washers with Verify™ damaged. Technology must recalibrate the Verify test plate after installing the new carrier.
Page 99 ® water buffer. Tween Some wells Insufficient BioTek offers a high-flow pump for assays left full. vacuum. using only water for the wash fluid. Contact BioTek for more information. Protocol settings Optimize protocols to improve evacuation not optimized.
Page 100 98 | 405 TS Getting Started Guide Fluid Delivery Problem Possible Cause What To Do Inlet tube not connected. Unable to Make sure all tubing is connected properly. dispense Check all external tubing for kinks or clogs. fluid. Clogged fluid filter.
Page 101 If the vacuum pump turns on, remove the vacuum tubing from the back of the instrument and put your finger over the port. If there is no vacuum, contact BioTek TAC. Uneven Clogged dispense tubes Remove and clean the washer manifold dispensing on the washer manifold.
Page 102 100 | 405 TS Getting Started Guide Fluid Leakage Possible Problem What To Do Cause Fluid Defective Contact BioTek TAC leaking seals. from Aspiration Check waste connector tubes; make sure they are manifold. tubes properly connected. only: If you are using an in-line vacuum filter, check the filter vacuum for clogging, and replace if necessary.
Page 103 Check for and remove any obstructions. Ensure the manifold is installed properly. Incorrect Make sure the washer manifold setting matches the manifold installed manifold (96-, 192--tube). selected. LHC: Tools>Instrument Utilities>Washer>Manifold Selection. Touch screen: Instrument>Options>Manifold Selection BioTek Instruments, Inc.
Page 104 102 | 405 TS Getting Started Guide Vacuum Pump Problems Problem Possible Cause What To Do Vacuum Vacuum pump is Flip the switch on the side of the vacuum pump pump does not turned on. to turn it on. not start, or...
Page 105: Specifications And Part Numbers
Specifications and Part Numbers For your convenience this section contains commonly sought reference information. Consult the operator's manual on CD for more complete information. Physical Specifications Performance Specifications Package Contents Optional Accessories...
Page 106 104 | 405 TS Getting Started Guide Physical Specifications Labware Microplates 96-well, 384-well that comply with SBS microplate standards 1-2004, 2-2004, 3-2004, and 4-2004. Microstrips 1 x 8, 1 x 12 Microwells Flat, round, "V” bottom Hardware & Environmental User Interface 5.7"...
Page 107 ≤ 3.0% CV when dispensing 300 µL per well of deionized water 96-Tube Manifold with 0.1% Tween 20, with FD&C #1 blue dye at rate 6 into a Costar 96-well flat-bottomed plate. The absorbance of the solution is read at 630 nm and 450 nm reference. BioTek Instruments, Inc.
Page 108 106 | 405 TS Getting Started Guide Dispense Precision ≤ 4.0% CV when dispensing 80 µL per well of deionized water 192-Tube Manifold with 0.1% Tween 20, with FD&C #1 blue dye at rate 7 into a Costar 384-well flat-bottomed plate. The absorbance of the solution is read at 630 nm and 450 nm reference.
Page 109: Package Contents| 107
Package Contents| 107 Package Contents Part numbers and package contents are subject to change and vary according to instrument model. Please contact BioTek Customer Care if you have any questions. Description Power cord (part numbers vary by country of use) Varies USB cable (USB Virtual COM Port Driver Software &...
Page 110 108 | 405 TS Getting Started Guide Waste and Dispense System Accessories Part Description Number Standard Vacuum Pump Systems: 1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles, including: 7100746 Waste: 4L bottles (2, one with sensor) 1170529 Dispense: 4L bottles with filters (2)
Page 111 1) Models with Buffer Switching receive a dispense system. Only a waste system must be ordered separately. 2) High-flow waste system may be necessary for 384-well washing with buffers not containing surfactant. 3) Direct Drain Waste System: use restricted to 96-tube single manifold models (i.e. 405 TS). BioTek Instruments, Inc.
Page 112: Optional Accessories
110 | 405 TS Getting Started Guide Optional Accessories General Instrument Accessories Description BioTek liquid testing solutions for Wetting 7773002 instrument qualification tests Agent Blue Test 7773001 Liquid Handling Control™ Software LHC2 BioStack™ Microplate Stacker and integration kit Biostack Installation-Operational-Performance Qualification 1170522 ...
Page 113 Vacuum Line Filter for Waste Tubing 48294 Vacuum Gauge/Regulator (For use with house vacuum.) 4030551 Silencing Muffler for Vacuum Pump (For use with P/N 7103024 01113 pump.) Fluid filter for dispense bottles (does not include stainless 01310 steel adaptor 1172031) BioTek Instruments, Inc.
Page 114 112 | 405 TS Getting Started Guide 405™ TS Microplate Washer...
Page 115: Index
Evacuation Efficiency AutoPrime Interval Average Residual Volume Flow Detection Fluid Detection BioStack Fluid Leaking Create a protocol Fluid Supply How to configure/operate Clean filter washer BioStack errors BioTek's Customer Resource Center BioTek's Ultrasonic Advantage General Settings Bottles Clean filter Connection Hardware...
Page 116 114 | I High Flow vacuum pump Port link Caution Power Supply Horizontal aspirate position Prevent fluid loss during wash Humidity limits Protein Preventing clogging Protocols Customize Instrument Settings Defining Instrument Utilities AutoPrime Qualification IQ-OQ-PQ Instrument Labware Rates Liquid test solutions Washer flow rates Low Flow Re-stack plates...
Page 117 Vacuum| 115 Vacuum Vacuum Dissipate Vacuum on based on volume Valve module installation Wash Gentle, cell wash Magnetic beads Polystyrene beads Washer Fixing problems Protocol parameters listing Washer Prime step Washer Settings Waste system Installation sensor BioTek Instruments, Inc.
Page 118 405 TS/LS Chemical Compatibility Chart Table 1: Material/Where Used List Material Where Used Inlet screen, feeder tube to manifold, vacuum 304 Stainless Steel switch Dispense and aspirate tubes, feeder tube to 316 Stainless Steel manifold, spring in bottle fittings Acetal...
Page 119: Chemical Compatibility
A ND D A ND ND This ratings information was obtained from several reputable sources and our own experience at BioTek, but your experience may differ due to variations in concentration, temperature, and other factors. Consult the reagent/solvent manufacturer before use to verify its compatibility with instrument components.
Page 120: 405 Ts Customer Training Guide
405 TS Customer Training Guide Instrument Hardware Onboard Software Review flow routing and tubing Show startup and priming procedure connection for Supply and Waste ( page Show Quick options: Prime Review Mist Shield installation and Wash cleaning Clean: Sonicate Manifold...
Page 121 LHC Software Show how to establish communication Review the options in the Target Instrument Settings Create and run a simple protocol Review the Instrument Utilities dialog options Preventive Maintenance Recommended maintenance schedule Decontamination page 77 Manifold cleaning procedures Cleaning in-bottle filter ( page 83 Stylus manifold Review programs:...
Page 122 Reference Material 405 TS Operator's Manual (on CD/USB stick) Touch screen Help LHC Help BioStack Operator's Manual...

Biotek 405 TS Getting Started Manual

Quick Start

Install the 405 TS

Basic Operation

Maintenance

Safeguards and Troubleshooting

Specifications and Part Numbers

Quick Links

Troubleshooting

Need help?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Biotek 405 TS

Summary of Contents for Biotek 405 TS

Table of Contents