Home Sensor Verification Errors (0400-0409) - Biotek Synergy HT Operator's Manual

Multi-mode microplate reader
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142
Appendix C: Error Codes
|
Code
Saturation transition failed in the Monochromator motor movement (light beam
0305
never found)
During the instrument initialization, the monochromator is homed by rotating the
monochromator mirror until the white light (full light) is detected. This requires a fully
functional Flash lamp/detection system.
Probable Causes:
• Defective Analog PCB.
• Defective Flash Lamp and or Flash Lamp Power Supply. (Inconsistent flashes; high
probability.)
• Defective motor/Power PCB.
• Defective monochromator (low probability).
• Order sorting filter wheel motor failed due to the bearings. The filter wheel does not move
to the open hold, allowing the monochromator to find home.
Probe height (Z) axis did not find home
0306
(This error code indicates old basecode. Recommend upgrading.)
Probable Causes:
• The optical flag is not adjusted properly.
• The Z-axis lead screw is no longer glued to the motor shaft.
• The upper fiber bundle is hitting the top of the shroud due to not being properly tied
down.
Probe changer motor did not find home
0307
(This error code indicates old basecode. Recommend upgrading.)
Probable Causes:
• An obstruction is preventing the motor from moving.
• The motor and/or motor drive circuit is defective.
• The sensor and/or sensor circuit defective.

Home Sensor Verification Errors (0400-0409)

These errors occur when the optical sensor is not found again within a range of
where it was last found. The causes can range from loose transmission components
to marginal adjustments. The last digit of the error identifies the axis in question.
Code
Carrier X-axis failed positional verify
0400
X-axis motor failed to get to the same position when moved a known number of steps from the
home position and back.
Probable Causes:
• The optical trigger flag has moved or is loose.
• Dirty rail, nylon bushings, or bearings.
• Carrier support pin is out of adjustment.
• Reference Field Change Notice L0030.
Description and Probable Causes:
Description and Probable Causes:
BioTek Instruments, Inc.

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Questions and answers

Luqman Babeer
April 25, 2025

I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you

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1 comments:
Mr. Anderson
May 12, 2025

To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:

1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.

If the issue persists, contact BioTek’s Technical Assistance Center.

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