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BioTek Synergy HT Manuals
Manuals and User Guides for BioTek Synergy HT. We have
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BioTek Synergy HT manual available for free PDF download: Operator's Manual
Biotek Synergy HT Operator's Manual (240 pages)
Multi-Mode Microplate Reader
Brand:
Biotek
| Category:
Laboratory Equipment
| Size: 8 MB
Table of Contents
Table of Contents
5
Contact Information
9
Revision History
10
Document Conventions
14
Intended Use Statement
15
Quality Control
15
Warranty & Product Registration
15
Warnings
16
Hazards and Precautions
16
Electromagnetic Interference and Susceptibility
19
User Safety
19
Safety Symbols
20
Chapter 1: Introduction
23
Synergy™ HT Multi-Mode Microplate Reader
24
Features
25
Package Contents
26
Optional Accessories
27
Product Support & Service
28
Technical Assistance Center (TAC)
28
Returning Instruments for Service/Repair
28
Contacting Biotek for Applications Support
28
Chapter 2: Installation
29
Product Registration
30
1: Unpack and Inspect the Reader
31
2: Remove the Shipping Panel
32
2: Remove the Shipping Panel
33
3: Remove the Microplate Carrier Shipping Screw
35
4: Install the Fluorescence Lamp Assembly
36
5: Select an Appropriate Location
37
6: Connect the Power Supply
38
7: Unpack and Inspect the Dispense Module
39
8: Install the Dispense Module
42
9: Connect the Host Computer
46
10: Install Software on the Host Computer
46
10: Install the Software on the Host Computer
46
11: Turn on the Reader
47
12: Establish Communication
48
Using Gen5
48
Using KC4
49
13: Run a System Test
50
14: Test the Injector System
50
14: Test the Injector System
51
Register with Biotek
52
Operational/Performance Qualification
52
Operational/Performance Qualification
53
Repackaging and Shipping Instructions
54
Chapter 3: Getting Started
57
Key Components
58
Power Switch, Carrier Eject Button, Microplate Carrier
58
Lamp Assembly and Filter Wheel Access
59
Excitation and Emission Filter Wheels
60
Installing the Time-Resolved Fluorescence Cartridge
63
Configuring the System for Luminescence Measurements
64
The External Dispense Module
65
Gen5 Software
68
Gen5™ Software
68
Viewing/Updating the Filter and Wavelengths Tables
68
Creating Protocols and Experiments
69
Controlling the Dispense Module
72
KC4 Software
74
KC4™ Software
74
Viewing/Updating the Filter and Wavelengths Tables
74
Creating Protocols
75
Reading Plates
77
Controlling the Dispense Module
78
Recommendations for Achieving Optimum Performance
81
Chapter 4: Instrument Qualification
83
Overview
84
Iq/Oq/Pq
84
Recommended Qualification Schedule
86
System Test
87
Description
87
Procedure
88
Absorbance Plate Test
92
Description
92
Test Plate Certificates
93
Setup: Gen5
94
Procedure: Gen5
95
Setup: KC4
96
Procedure: KC4
96
Results & Troubleshooting Tips
100
Absorbance Liquid Tests
102
Stock Solution Formulation
103
Liquid Test 1
104
Liquid Test 2
105
Liquid Test 3
108
Fluorescence Tests
111
Required Materials
112
Test Solutions
113
Procedure
114
Results Analysis
114
Troubleshooting
115
Pipette Map
116
Gen5 Protocol Reading Parameters
117
KC4 Protocol Reading Parameters
118
Fluorescence Tests Using Methylumbelliferone
120
Dispense Module Tests
124
Required Materials
125
Test Solution Recipes
126
Test Setup: Gen5
127
Test Setup: KC4
127
Test Procedure: Gen5
128
Test Procedure: KC4
130
Results Analysis
131
Creating Test Protocols Using Gen5
132
Creating Test Protocols Using KC4
137
Dispense Accuracy & Precision Test Worksheet
143
Dispense Accuracy & Precision Tests Worksheet
143
Chapter 5: Preventive Maintenance
145
Recommended Maintenance Schedule
146
Overview
146
Dispense Module
146
Schedule
146
Warnings & Precautions
148
Cleaning Exposed Surfaces
149
Inspecting/Cleaning Excitation and Emission Filters
150
Flushing/Purging the Fluid Path
151
Running a Dispense Protocol (Optional)
152
Emptying/Cleaning the Tip Priming Trough
154
Cleaning the Priming Plate
154
Cleaning the Internal Components
155
Required Materials
156
Removing the Reader's Shroud
157
Removing the Internal Tubes and Injector Heads
159
Cleaning the Internal Tubes and Injector Heads
162
Cleaning the Optical Probes
163
Cleaning the Reader's Internal Surface
171
Reassembling the Components
172
Performance Check
173
Appendix A: Decontamination
175
Purpose
176
Required Materials
177
Procedure for Models Without Injectors
178
Routine Procedure for Models with Injectors
179
Clean Exposed Surfaces
179
Decontaminate the Fluid Lines
180
Rinse the Fluid Lines
181
Clean the Internal Tubing and Injector Heads
181
Clean the Tip Prime Trough and Priming Plate
182
Clean the Tip Priming Trough and Priming Plate
182
Alternate Procedure for Models with Injectors
183
Appendix B: Computer Control
185
Appendix C: Error Codes
187
Error Codes
188
Fatal Errors
189
Non-Fatal Errors
190
Home Sensor Initial Find Errors (0100-0307)
190
Home Sensor Verification Errors (0400-0409)
194
Saturation Errors (0500-0616)
196
Absorbance Reader Noise Errors (0700-0A16)
202
Internal Self-Test Errors (0D00-2918)
206
Other Errors (2A00-4000)
218
Status String Format
224
Appendix D: Specifications
229
Hardware and Environmental
230
Microplates
230
Specifications
230
This Appendix Contains Biotek's Published Specifications for the
230
Absorbance
231
Fluorescence
233
Incubation
235
Injector Model
235
Shake
235
Appendix E: Instrument Dimensions for Robotic Interface
237
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