Biotek Synergy HT Operator's Manual page 118

94
Chapter 4: Instrument Qualification
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Define three Assay Control IDs and assign them to the wells shown here:
•
Disp_80
•
Disp_20
•
Disp_5
Refer to the Gen5 Help system for detailed instructions.
Add Data Reduction Steps
Each Read step is performed using two wavelengths, so you'll need to create two
data reduction steps to calculate the Delta OD values.
1. Select
select
2. Within this dialog, click the
click the
•
•
3. Click
4. In the
OD 80 ul_Disp 1".
5. Clear
6. In the
to H4
7. Click
8. Create another Transformation similar to the above, with these
characteristics:
•
•
•
•
9. When you're finished, the Data Reduction Steps list will show two Delta
OD transformations.
10. Click
11. Save the protocol with an identifying file name, such as "Synergy HT
Dispenser 1.prt".
A1 to H4
A5 to H8
A9 to H12
Protocol > Data Reduction
Transformation ).
DS2 radio button (see below).
Set the Data In for DS1
Set the Data In for DS2
OK to return to the dialog.
New Data Set Name
Use single formula for all wells.
Current Formula
to assign the formula.
OK to add the transformation to the Data Reduction list.
DS1 set to the 20 and 5
DS2 set to the 20 and 5
New Data Set Name
Formula applied to wells
DS1-DS2
OK to close the Data Reduction dialog.
and select
Select Multiple Data Sets
to the 80 µL Read step at
to the 80 µL Read step at
field, type an identifying name such as "Delta
field, type DS1-DS2
µL Read step at 630
µL Read step at 750
resembling 'Delta OD 20 and 5 ul_Disp <#>'
to
A5 H12
Custom (for Gen5 1.x users,
button and then
405 nm.
750 nm.
and then highlight wells
nm
nm
BioTek Instruments, Inc.
A1