Biotek Synergy HT Operator's Manual page 12

Multi-mode microplate reader
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viii
Preface
|
Rev
Date
Changes
F
7/2004
General: Edited and reformatted text according to new template. Added
photographs for clarification as needed.
Chapter 1: Updated Optional Accessories list.
Chapter 2: Updated text and graphics to describe/illustrate: Removal of the front
injector. Redesign of the priming plate, to limit splatter. Improved system design
to reduce need for periodic maintenance. Elimination of the right-front tip priming
trough, and redesign of the left-rear trough. Improved bottle holder setup.
Chapter 3: Updated diagram showing removal of Dispense Module from inner
shipping box. Updated procedure for setting up Dispense Module on the Injector
Model.
Chapter 4: Removed instructions for setting injector position.
Chapter 6: Added preventive maintenance procedure for periodic cleaning of the
top/bottom fluorescence optical probes and the absorbance read channel optical
path.
Appendix A: Updated drawing of tip priming trough and priming plate.
G
8/2005
Updated Warranty information.
Moved "Specifications" from Chapter 1 to Appendix D. Corrected operating
temperature (18-40°C) and injector accuracy (±1 µl at 5-50 µl) to match
published specifications.
Removed Chapter 2, "Instrument Description" and distributed the information and
photos among the remaining chapters.
Reorganized the flow of the "Installation" chapter to better represent actual
practice. Added test to verify the injector system setup.
Changed the former "Getting Started with KC4" chapter to a broader "Getting
Started" chapter that includes information on the key instrument components.
Added new topic for configuring the system for luminescence reads.
Renamed the "Performance Verification/Qualification Tests" chapter to
"Instrument Qualification." Replaced former Dispense Precision & Accuracy Tests
with new tests that use a single green dye solution and a single microplate.
Restructured the "Preventive Maintenance" chapter to better represent actual
practice. Added a recommended maintenance table for models without injectors.
Added new photos to help with identification of the various components.
Updated the "Error Codes" appendix with recent information.
Additional minor corrections and improvements throughout.
H
5/2006
Redesigned the front cover. Removed unnecessary Warranty information; a
Warranty card ships with every instrument. Added warning to shut down
instrument and wait for the fluorescence lamp to cool down before replacing it.
Added the PN for a replacement fluorescence lamp (7080500).
For models with injectors: Simplified the installation and setup steps for the
Dispense Module. Added recommendation to set a tip prime volume equal to the
per-well dispense volume for volumes < 20 µl.
Updated Absorbance Plate Test instructions related to Peak Wavelength, to
support the modified 7-filter test plate.
Simplified the process for creating Titration Dyes for the Fluorescence (SF)
Sensitivity Test. Added information to the pass/fail criteria table for the (SF)
Sensitivity Test.
Clarified that for models without injectors, the reader's internal chamber and
optical probes are not user-accessible for cleaning.
Updated the "Error Codes" appendix with recent information.
Additional minor cosmetic changes throughout.
Added/modified instructions throughout to support Gen5™, including:
Chapter 2, Installation - Added instructions for installing software, establishing
BioTek Instruments, Inc.

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Questions and answers

Luqman Babeer
April 25, 2025

I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you

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1 comments:
Mr. Anderson
May 12, 2025

To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:

1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.

If the issue persists, contact BioTek’s Technical Assistance Center.

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