Biotek BioStack 4 Operator's Manual
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Biotek BioStack 4 Operator's Manual

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Summary of Contents for Biotek BioStack 4

  • Page 1 Microplate Stacker BioStack ™ Operator’s Manual...
  • Page 3 BioStack™ Microplate Stacker Operator's Manual © 2018 BioTek Instruments, Inc. PN 7311000 Rev AH...

  • Page 4: Notices

    BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 5: Table Of Contents

    USA FCC CLASS A xvii Canadian Department of Communications Class A xvii User Safety xviii Safety Symbols BioStack 3 and BioStack 4: Secure Gripper Before Moving Introduction Introducing the BioStack Product Description BioStack 4: Notes about Handling Lidded Plates Package Contents Optional Accessories...
  • Page 6 | BioStack Operator's Manual Test Plates for Compatibility—BioStack 4 Only Connect to Power Connect to Computer Connect to Liquid Handler Where to Go Next Repacking the BioStack Install the Shipping Hardware—BioStack2WR Install the Shipping Hardware—BioStack 3 Install the Shipping Hardware—BioStack 4 Repacking—Install Shipping Panel...

  • Page 7: Notices| V

    BioStack Sensor Numbers BioStack Motor Numbers Interfacing Instrument Numbers Plate Stacked Height Change Setting for Low-Profile Plates Loosen Plate Carrier Set Screws - BioStack 4 Only Adjust the Stack Dogs Install the Barcode Scanner Barcode Scanner Overview Install the Barcode Scanner...

  • Page 8: Contact Information

    Winooski, Vermont 05404-0998 USA Global Service and Support BioTek instrument service and repair is available worldwide at several of BioTek’s International Service Centers and in the field at your location. To arrange for service or repair, contact the office nearest you; visit www.biotek.com...

  • Page 9: Revision History

    07/2006 Added instructions for installing/operating the BioStack with the Synergy 2. 12/2006 Added instructions for installing and operating the BioStack with the NanoQuot. In Chapter 5 and Appendix B: Added the Barcode Scanner Test as an optional IQ and OQ test. BioTek Instruments, Inc.
  • Page 10 viii | BioStack Operator's Manual Rev Date Changes 06/2007 Added instructions for installing and operating the BioStack with the Synergy 4. Updated error code information. Incorporated manual update revision H1 as Appendix C, Reconfiguration of the BioStack. Added Appendix D, Required Software Versions. 08/2007 Added references to the Liquid Handling Control (LHC) Software throughout the manual, and instructions for installation/operation of the software for PC control of the BioStack and ELx405.
  • Page 11 Cytation 3. Corrected or improved content based on suggestions from field staff, including deleting the statement that barcode labels can be purchased from BioTek. Added references to the rubber band that secures the BioStack3's wrist during shipping. Recommended using rubber bands to...
  • Page 12 6/2014 Added support for new instruments: Epoch2 and Synergy HTX. Added a warning that the PowerWave HT is not compatible with the BioStack 4 and a "strong recommendation" to measure the plate and lid type before use to verify they meet the minimum requirements for de-lidding operations with the BioStack 4.

  • Page 13: Document Conventions

    The BioStack Microplate Stacker is an automated microplate stacking system that is designed to systematically transfer a stack of microplates to and from compatible BioTek laboratory instruments wherever washing, dispensing, pipetting, or reading of multiple microplates is performed. If the instrument has an “IVD” label it may be used for clinical and non-clinical purposes, including research &...

  • Page 14: Quality Control

    Warranty and Product Registration Please take a moment to review the Warranty information that shipped with your product. Please also register your product with BioTek to ensure that you receive important information and updates about the product(s) you have purchased.

  • Page 15: Hazards

    . Serious eye injury may occur if you stare directly into the laser beam of the barcode scanner during operation of the scanner. This hazard is noted by the symbol shown here. Do not look directly into the laser beam during operation of the scanner.     BioTek Instruments, Inc.

  • Page 16: Precautions

    Precautions The following precautions are provided to help avoid damage to the instrument(s): Caution: Service . The instrument should be serviced by BioTek-authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components. Caution: Spare Parts.
  • Page 17 Caution: Electromagnetic Compatibility . Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), because these may interfere with the proper operation. BioTek Instruments, Inc.

  • Page 18: Ce Mark

    xvi | BioStack Operator's Manual CE Mark Based on the testing described below and information contained herein, this instrument bears the CE mark. Note: Refer to the Declaration of Conformity for specific details. Directive 2014/30/EU: Electromagnetic Compatibility Emissions—Class A The system has been type-tested by an independent, accredited testing laboratory and found to meet the requirements of EN 61326-1: Class A for Radiated Emissions and Line Conducted Emissions.

  • Page 19: Directive 2012/19/Eu: Waste Electrical And Electronic Equipment

    Department of Communications. Le present appareil numerique n'émet pas de bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada. BioTek Instruments, Inc.

  • Page 20: User Safety

    xviii | BioStack Operator's Manual User Safety This device has been type-tested by an independent laboratory and found to meet the requirements of the following:   “Safety requirements for electrical Underwriters Laboratories UL 61010-1 equipment for measurement, control and laboratory use; Part 1: general requirements.”...

  • Page 21: Safety Symbols

    Attenzione, rischiodiscossaelettrica Zerquetschens und Klemmen Precaución, riesgo del machacamiento y sejeción Attenzione, rischiodischiacciareedintrappol arsi Warning, hot surface Warning, potential biohazards Attention, surface chaude Attention, Warnen, heißeOberfläche risquesbiologiquespotentiels Precaución, superficiecaliente Warnung! Attenzione, superficiecalda MoeglichebiologischeGiftstoffe Atención, riesgosbiológicos Attenzione, rischiobiologico BioTek Instruments, Inc.

  • Page 22: Biostack 3 And Biostack 4: Secure Gripper Before Moving

    BioStack 3 and BioStack 4: Secure Gripper Before Moving Note: Do not let the gripper flop around! Before moving the BioStack 3 or BioStack 4, secure the gripper with a rubber band or tape to prevent it from losing its alignment. A complex recalibration is required if it is knocked out of position.

  • Page 23: Introduction

    Chapter 1 Introduction Thank you for purchasing the BioStack™ Microplate Stacker. This chapter describes its features and specifications and includes important contact information. Introducing the BioStack Package Contents Optional Accessories Specifications  ...
  • Page 24 2 | Chapter 1: Introduction Introducing the BioStack This manual covers the BioStack (BIOSTACK2WR), BioStack 3 (BIOSTACK3WR), and BioStack 4 (BIOSTACK4). Unless otherwise specified, the term BioStack refers to all models. BioStack 3 shown without stacks Side view 1. Gripper—arm with wrist and gripper 2.

  • Page 25: Product Description

    Load Plates Using the Pedestal on page Important: Review the Wrist Angle Setting Requirements on page 26 to determine if you need to change the wrist angle setting to interface correctly with your BioTek reader or liquid handler. Product Description The BioStack Microplate Stacker is a simple and cost-effective solution for benchtop automation of BioTek’s microplate instrumentation.

  • Page 26: Biostack 4: Notes About Handling Lidded Plates

    Reliable transport with microplate gripped from below during transfers Onboard self-diagnostics ensures trouble-free operation BioStack 4 Only: Handles microplates with lids: removes the lid before plate transfer to the attached instrument, and replaces the lid after retrieval from the instrument, except in "stay-on lid"...
  • Page 27 Another limiting factor is the total weight of the filled plates in the stack cannot exceed 7.0 lbs (3.2 kg). When processing plates with lids the BioStack 4 performs several extra error-checking movements, using its fingers to determine if the expected labware, i.e., a plate, lid, or combination, is present.

  • Page 28: Package Contents

    6 | Chapter 1: Introduction Package Contents Part numbers and package contents are subject to change and vary according to instrument model. Please contact BioTek Customer Care if you have any questions. BioStack Microplate Stacker (PN BIOSTACK2WR, BIOSTACK3WR, BIOSTACK4) Power supply: 24 volt (PN 01281)
  • Page 29 Integration Kits for use with interfacing instruments: Instrument Integration Kit 405 TS/LS PN 7310040 Cytation 1/3/5 PN 7310053 EL406 PN 7310042 ELx405 PN 7310041 PN 7310049 EpochR BIOSTACK2WR: PN 7310032 BIOSTACK3WR/BIOSTACK4: PN 7310043 Epoch 2 PN 7310054 MicroFill* PN 7310012 BioTek Instruments, Inc.
  • Page 30 Synergy Neo and Synergy PN 7310055 Neo2‡ *Compatible with BIOSTACK2WR only. ‡ Compatible with BioStack 4 only and uses its own barcode scanner, i.e., not compatible with the BioStack barcode scanner listed above. ¥ Not compatible with BIOSTACK4.   BioStack™ Microplate Stacker...

  • Page 31: Specifications

    (0.015") at the corners (0.015") at the corners 14.60 mm (0.575") Typical 96-, 384-, 1536-well plates: Height: 22.0 mm (0.866") using BioStack 4 16.9 mm (0.665") plate + lid combined: Lidded Plate Stacks. plate max is 14.60 mm, except in Stay- on Lid Mode.

  • Page 32: Stay-On Lid Mode (Biostack 4 Only)

    23.2 mm (0.913") Max overall height Stay-On Lid Mode (BioStack 4 Only) When these conditions are met the BioStack 4 handles certain low-density plates with lids. This mode offers the ability to keep the lid on the plate for the duration of the protocol.
  • Page 33 Specifications | 11 Minimum software versions: BioStack 4 basecode: PN 1230200 version 1.05 (or higher) Gen5: version 2.07 (or higher) LHC: version 2.18 (or higher) Note: Gen5 TS (for touch screen models) does not support stay-on lid mode. Certain 6-, 12-, 24-, and 48-...
  • Page 34 12 | Chapter 1: Introduction Parameters are defined to support it: Fill the checkbox in Advanced Options. Plate Type records for Nunc 6-, 12-, 24-, and 48-well plates and lids that meet these requirements are ready for selection, e.g., "Nunclon 24 well." You can create or modify the plate type record of other plates that meet the requirements.

  • Page 35: Installation

    Upgrade Scenarios Unpack and Inspect the Instrument Setting Up the BioStack Remove the Shipping Hardware—BIOSTACK2WR Remove the Shipping Hardware—BioStack 3 Remove the Shipping Hardware—BioStack 4 Dip Switch Settings Connect to Power Where to Go Next Repacking the BioStack Install the Shipping Hardware—BioStack2WR Install the Shipping Hardware—BioStack 3...
  • Page 36 If you purchased the BioStack to interface with an existing instrument, you may need to upgrade the hardware and/or software on that instrument. Refer to the table below and contact BioTek TAC with any questions. Minimum computer control software for:...
  • Page 37 Unpack and Inspect the Instrument Save all packaging materials. If you need to ship the instrument or accessories to BioTek for repair or replacement, you must use the original packaging. Using other forms of commercially available packaging is not recommended and can void the warranty.
  • Page 38 16 | Chapter 2: Installation  1. Grasp the handles on the shipping panel to lift the instrument from the inner box.  2. Gently lay the BioStack on its side on the work surface, so the panel hangs over the edge.  3.
  • Page 39 80% at temperatures up to 31° C decreasing linearly to 50% relative humidity at 40° C.   : Efficient microplate transporting may be affected by extraneous Dust particles (such as dust) on the carrier’s linear ways. A clean work area is necessary to ensure smooth plate transporting. BioTek Instruments, Inc.
  • Page 40 18 | Chapter 2: Installation Remove the Shipping Hardware—BIOSTACK2WR Warning: Remove the shipping hardware before turning on the BioStack. : You need a standard screwdriver, a small Phillips-head screwdriver, and Tools 3/32" hex wrench. Shipping hardware in the BioStack's interior (BIOSTACK2WR)  1.
  • Page 41  5. Use the 3/32 hex wrench to remove the wrist shipping block.  6. Reinstall the wrist/gripper cover.  7. Store the screws with the other packaging materials in the shipping boxes. BioTek Instruments, Inc.

  • Page 42: Remove The Shipping Hardware-Biostack 3

    20 | Chapter 2: Installation Remove the Shipping Hardware—BioStack 3 Warning: Remove the shipping hardware before turning on the BioStack. : You need a standard screwdriver and a small Phillips-head screwdriver. Tools One shipping bracket wraps around the front Another shipping block sits inside  1.
  • Page 43  5. Remove the rubber band that holds the rotational wrist secure. Note: Do not let the gripper flop around! Before moving the BioStack 3 or BioStack 4, secure the gripper with a rubber band or tape to prevent it from losing its alignment. A complex recalibration is required if it is knocked out of position.
  • Page 44 22 | Chapter 2: Installation  7. Store all the shipping hardware and screws with the other packaging materials in the shipping boxes and keep for future use. BioStack™ Microplate Stacker...

  • Page 45: Remove The Shipping Hardware—Biostack 4

    Remove the Shipping Hardware—BioStack 4 | 23 Remove the Shipping Hardware—BioStack 4 Warning: Remove the shipping hardware before turning on the BioStack. : You need a standard screwdriver and a small Phillips-head screwdriver. Tools One shipping bracket wraps around the front and attaches to the inside.
  • Page 46 24 | Chapter 2: Installation Remove the gripper cover plate Note: Screw the screws into the shipping bracket for storage.  5. Remove the plate carrier shipping screw.  6. Returning to the front of the BioStack, remove the rubber band that holds the rotational wrist secure.
  • Page 47  8. Install the small cover plate you removed from the shipping bracket on top of the gripper, using the small Phillips-head screwdriver. Note: Do not let the gripper flop around! Before moving the BioStack 3 or BioStack 4, secure the gripper with a rubber band or tape to prevent it from losing its alignment.

  • Page 48: Wrist Angle Setting Requirements

    26 | Chapter 2: Installation  9. Locate and install the gripper arm cover using the small Phillips-head screwdriver.  10. For safekeeping, screw all the screws and washers, the small shipping brackets and the large shipping block onto the large shipping bracket. Store the shipping hardware with all the other packaging materials in the shipping boxes and keep for future use.

  • Page 49: Degree Orientation For Liquid Handlers

    0° orientation. This may be necessary to fit both the instrument and the BioStack in a safety hood, for example. Or when bench space is limited. This option is not supported with BioStack 3 or BioStack 4. Change the dipswitch setting as described below. Attach the BioStack aligning plate to the instrument's aligning plate in the front holes provided, as shown here: BioTek Instruments, Inc.

  • Page 50: Dip Switch Settings

    28 | Chapter 2: Installation 0° Integration Position Dip Switch Settings Two sets of dip switches on the BioStack control variable options, e.g., to change the wrist angle, use 50-plate stacks, or install the barcode scanner. Dip switch 1 and 2 Two arrays of dip switches, switch 1: SW1 and switch 2: SW2.

  • Page 51: Change Setting For Wrist Angle

    SW2-3 Closed and SW2-4 Closed = -90° Wrist Angle Change Setting for Wrist Angle Most BioTek instruments require the BioStack wrist to be set at a specific angle. Follow these instructions to change the wrist angle dip switch setting for your instrument.

  • Page 52: Change Setting For 50-Plate Stacks

    (the right-most dip switch) to its SW-2 Open position, away from the instrument.  4. Replace the plug. Test Plates for Compatibility—BioStack 4 Only Perform this simple test to make sure your microplates are compatible with the BioStack 4: BioStack™ Microplate Stacker...

  • Page 53: Connect To Power

    If the plate fits too snugly in the carrier, you can loosen screws in the side of the carrier to give the plate more room: See Loosen Plate Carrier Set Screws - BioStack 4 Only on page 90 Strongly recommended: Measure your plate and lid type to verify they meet the minimum requirements before use: See Specifications on page 9.

  • Page 54: Connect To Computer

    When operating the BioStack with a reader (except touch screen instruments) or with a liquid handler being controlled by LHC (BioTek's Liquid Handling Control software), connect the BioStack to the host computer  1.

  • Page 55: Connect To Liquid Handler

    BioStack. Make sure there is no plate on the carrier, and check for any obstructions. Ensure that all of the shipping hardware has been removed. If you cannot resolve the problem, contact BioTek’s Technical Assistance Center.
  • Page 56 Communication is successful if the BioStack basecode software version (the first number), interface definition version (the second number), and checksum are displayed. If communication is not successful, look up the error code: Communication Error . Contact BioTek if you cannot resolve the problem. Codes on page 74 BioStack™ Microplate Stacker...

  • Page 57: Where To Go Next

    Qualify the BioStack The Qualification chapter beginning on page 69 for recommended procedures to qualify the initial and ongoing performance of the BioStack Interpret error codes The Troubleshooting chapter beginning on page 73 or resolve problems BioTek Instruments, Inc.

  • Page 58: Repacking The Biostack

    Repacking the BioStack Prior to sending your instrument to us for repair, log into the Customer Resource Center (www.biotek.com) to submit a Service Request for a Service Call Notice (SCN) number. Your instrument's serial number is needed to process an SCN.

  • Page 59: Install The Shipping Hardware-Biostack2Wr

     2. Remove the gripper arm cover. Place the screws in the plastic bag (#98170) and put the bag inside of the arm cover. Put the arm cover with the screws into the bubble bag (#91083). BioTek Instruments, Inc.

  • Page 60: Install The Shipping Hardware—Biostack 4

    Next step: Repacking—Install Shipping Panel on the facing page Install the Shipping Hardware—BioStack 4 Reverse the process of uninstalling the shipping hardware. Refer to the photos page 23 : You need a standard screwdriver and a small Phillips-head screwdriver.

  • Page 61: Repacking-Install Shipping Panel

    Install the Shipping Hardware—BioStack 4 | 39    6. Wrap the wrist with a rubber band to secure it for shipping. Loop the rubber band around posts on either side of the wrist.  7. Install the shipping block inside the BioStack and the plate carrier screw.
  • Page 62  6. Put the corner blocks on the inner shipping box and put it into the outer shipping box. Tape the box closed.  7. Write the work order number in large, clear letters on the outside of the box. Ship the box to BioTek. BioStack™ Microplate Stacker...

  • Page 63: Operation

    Chapter 3 Operation This chapter provides instructions for controlling the BioStack. Before You Start Load Plates into Input Stack Processing Microplates Processing Plates with Lids  ...
  • Page 64 42 | Chapter 3: Operation Before You Start If you have not already done so: Install the BioStack with the interfacing instrument using the instrument-specific instructions provided. If the instruments have been moved since installation, perform the Verify Test to re- verify alignment.
  • Page 65 (and plates taller than 14.6 mm without lids). : use plates with flat, vertical sidewalls to ensure the BioStack Barcode Scanner labels will lie flat upon the surface. See Preparing and Attaching Barcode Labels on page 99 BioTek Instruments, Inc.

  • Page 66: Load Plates Using The Pedestal

    44 | Chapter 3: Operation Load Plates Using the Pedestal BioTek provides a handy tool for loading plates into and removing them from a stack. The pedestal, PN 7312083, gives you better access to the plates when loading the stack, helping to ensure they sit properly and evenly.

  • Page 67: Supported Microplate Types And Dimensions

    Stacked height is the plate height minus the base recess. Most plates have a base recess of 1.0 mm and a skirt height of 2.4 mm, 6.1 mm, or 7.6 mm. See Plate Stacked Height on page BioTek Instruments, Inc.

  • Page 68: Processing Microplates

    46 | Chapter 3: Operation Less-rigid polypropylene, low-profile PCR plates must have sealing tape applied over the entire top surface. BioStack 4 is designed to better handle plate warping than other models, nevertheless, these plates may cause problems, especially after thermocycling.

  • Page 69: Stopping Plate Processing

      to continue execution where the instrument left off and complete RESUME the protocol. If a plate is being transferred when you press Stop, the keypad displays a "Please Wait" message until the current operation is completed. BioTek Instruments, Inc.
  • Page 70 Resume the BioStack: useful for troubleshooting or restarting a process. Home : If problems arise, BioTek TAC may find it useful to review the BioStack Details processing details collected by Gen5 to resolve the issue. Note: You may need to manually remove a plate, lid, or both, from the BioStack if its operation is stopped mid-transfer.

  • Page 71: Restack The Plates

    Make sure the stack is labeled "Tall Lidded Plate Stack." Use these stacks for tall microplates (>14.6 mm) without lids, also. You can use any stacks when not processing lids or tall plates. See also Stay-On Lid Mode (BioStack 4 Only) on page When filling the stack, ensure that every plate has a lid.

  • Page 72: Plate De-Lidding: How It Works

    Plate De-Lidding: How It Works BioStack 4 Only. Here is an overview of how the BioStack 4 handles plates with lids. Like all BioStack processing, plates are pulled from the bottom of the input stack, i.e., the bottom plate is the first one transferred, and returned to the bottom of the output stack.

  • Page 73: Prevent Condensation On Plate Lids

    Reading plates with lids: The Gen5 option "Keep lids on during read" performs differently depending on the plate type. The BioStack 4 can keep the lids on plates throughout processing only when they meet the requirements of Stay-On Lid Mode (BioStack 4 Only) on page 10.

  • Page 74: Plate Lid Definition Files

    To distinguish between a microplate and its lid, the BioStack must know the precise dimensions of each. BioTek provides plate lid definition files for the most popular microplate types. You must create your own plate lid definition files for plate types not provided.

  • Page 75: Microplate And Lid Dimensions

    Use the plate height and width values provided by the manufacturer, and any other relevant measurements included in their data sheets. These dimensions are used to calculate the gripper height and finger positions, which are critical to proper operation. BioTek Instruments, Inc.

  • Page 76: Plate Lid Advanced Dimensions

    Plate Lid Advanced Dimensions Typically, the BioStack 4 will smoothly process plates with lids using the default values for these parameters. However, if you experience processing problems, like dropped lids, minor adjustments to these values may restore expected performance.
  • Page 77 If this value is zero, then the entire vertical move is fast. Stay-on lid: Identifies qualifying labware, i.e., certain low-density microplates that meet dimension requirements. Close Dog Adjust BioTek Instruments, Inc.
  • Page 78 56 | Chapter 3: Operation Lid Point Grip and Lid Overlap Loose Grip Offset Plate Clearance BioStack™ Microplate Stacker...

  • Page 79: Start Up The Biostack

    Touch screen 1. Select 1. Press 1. At the Home screen, press Tools>BioStack UTIL>Setup Instrument. Utilities. Menu. 2. Select Next > BioStack 2. Click Reset press Get Basecode Version. 2. Select BioStack. BIOSTK > ALIGN > HOME. BioTek Instruments, Inc.
  • Page 80 BioStack. Make sure there is no plate on the carrier, and check for any obstructions. Ensure that all of the shipping hardware has been removed. If you cannot resolve the problem, contact BioTek’s Technical Assistance Center for guidance. BioStack™ Microplate Stacker...

  • Page 81: Maintenance

    Chapter 4 Maintenance Properly maintaining the BioStack is the key to reliable performance. Overview Recommended Maintenance Schedule Warnings and Precautions Required Materials Routine Cleaning Procedure Decontamination Tools and Supplies Decontaminate the BioStack Cleaning and Lubricating the Linear Ways  ...
  • Page 82 60 | Chapter 4: Maintenance Overview Maintenance comprises a set of procedures to be performed regularly to maintain equipment in top condition. During normal operation, debris and dust from plate wear can accumulate on the microplate stacks, carrier surface, and other plate transport components.
  • Page 83 Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazard(s) they handle                                                ...
  • Page 84 70% isopropyl alcohol or ethanol (for decon)   For barcode scanner: lens cleaning solution and lens tissue. For cleaning/lubricating the linear ways:   Grease kit, BioTek PN 7110017 (provided)   Clean, lint-free disposable towels   Lint-free swabs or wipes  ...
  • Page 85  6. If detergent was used, wipe all surfaces with a cloth moistened with water.  7. Use a clean, dry cloth to dry all wet surfaces.  8. Reassemble the instrument. BioTek Instruments, Inc.

  • Page 86: Decontamination

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazards they handle.

  • Page 87: Tools And Supplies

     7. Wait 20 minutes. Moisten a cloth with deionized or distilled water and wipe all surfaces that have been cleaned with the alcohol.  8. Use a clean, dry cloth to dry all wet surfaces. BioTek Instruments, Inc.

  • Page 88: Cleaning And Lubricating The Linear Ways

    Read the Warnings & Precautions on page 61. Note: Do not let the gripper flop around! Before moving the BioStack 3 or BioStack 4, secure the gripper with a rubber band or tape to prevent it from losing its alignment. A complex recalibration is required if it is knocked out of position.
  • Page 89 (If the barcode scanner is installed, turn the BioStack on the side without the scanner.)  3. Using lint-free swabs or wipes, remove old, possibly contaminated grease from the three linear ways.  4. Allow these parts to dry completely. BioTek Instruments, Inc.
  • Page 90 If the Verify Test passes, the BioStack is ready to resume normal operation.   If the Verify Test fails, realign the gripper with the plate carrier using the Alignment Utility and rerun the Verify Test. If the test continues to fail, contact BioTek’s Technical Assistance Center. BioStack™ Microplate Stacker...

  • Page 91: Qualification

    Chapter 5 Qualification This chapter provides instructions for periodically testing the instrument to verify that it meets performance specifications. Overview Recommended Qualification Schedule Installation Qualification Operational Qualification Procedure...
  • Page 92 An instrument qualification package (PN 7310530) for the BioStack is available for purchase. The package contains detailed procedures for performing Installation/Operational Qualification (IQ/OQ) and Preventive Maintenance (PM), and extensive checklists and logbooks for recording data and results. Contact BioTek for more information. Recommended Qualification Schedule TASKS/TESTS  ...
  • Page 93 ** Perform the Instrument Alignment and Verify Test whenever the BioStack is moved from one location to another. Performance Qualification (PQ) procedures are not required for the BioStack. Performing chemistry testing on the microplates is not applicable to the BioStack. ‡ Install the Barcode Scanner on page 94 BioTek Instruments, Inc.
  • Page 94 72 | Chapter 5: Qualification Installation Qualification Installation Qualification confirms that the BioStack and its components have been supplied as ordered, and ensures that they are assembled and configured properly for your lab environment. See Recommended Qualification Schedule on page 70 for the recommended IQ tasks and tests.

  • Page 95: Troubleshooting & Error Codes

    Chapter 6 Troubleshooting & Error Codes This chapter provides guidelines for error recovery and troubleshooting performance problems. Troubleshooting Communication Error Codes...

  • Page 96: Troubleshooting

    Plate Stacked Height: when controlling the BioStack 3 or BioStack 4 (and not processing lids) with Gen5, LHC or touch screen, define a custom plate stacked height to improve the gripper's position on the plate.
  • Page 97 Try restarting the instrument. Try restarting the application. -509 PC software received back more data than it was expecting. Unlikely to be seen. It is expected that a –506 would be sent instead. Try restarting the application. BioTek Instruments, Inc.

  • Page 98: Biostack Instrument Errors

    76 | Chapter 6: Troubleshooting & Error Codes Communication Errors -510 Invalid message object. This error indicates that either the instrument basecode software or the PC software doesn’t recognize the message sent. This is unlikely to be seen, but could possibly happen if the software fell out-of-sync with the characters sent back and forth.
  • Page 99 Autocal jig placement error. The autocal jig was installed incorrectly. Position the jig correctly and retry. 0700 Failed interface configuration checksum test. Nonvolatile configuration data associated with the external instrument interface may be missing or corrupt. Turn the instrument off/on. If the error persists, contact BioTek TAC. BioTek Instruments, Inc.
  • Page 100 Max requests currently running. A communication error—see error 1300. 1501 Internal 5V power supply test error. Check connections to the external power supply. Turn the instrument off/on, and if the error persists, contact BioTek TAC. 1502 External power supply test error. Check connections to the external power supply.
  • Page 101 Inaccurate values in the Plate Type or Plate Lid Definition files. Defective microplate and/or lid, e.g. damaged edge. 1706 Maximum number of plates reached. Restacking stopped because the plate count has exceeded the number of plates that can fit in the stack. Contact BioTek TAC for assistance. BioTek Instruments, Inc.
  • Page 102 170B Lid geometry not set. Command to use lids cannot be completed because a lid definition (plate type) file is missing or unselected. Contact BioTek TAC. 180# The given motor number was not present. # = motor; see page 87 for a list of motor numbers.
  • Page 103 See Wrist Angle Setting Requirements on page 26 and make sure the dip switch setting for wrist angle matches your instrument requirement. Contact BioTek TAC if problems persist. 2600 A gripper interface dimension is out-of-range at power up. Rerun the Instrument Alignment Utility to clear the error.

  • Page 104: Plate Lid Definition Errors

    AE8# The motor profile resolution is invalid for phase correction. # = motor; see page 87 for a list of motor numbers. Contact BioTek TAC. Plate Lid Definition Errors When processing microplates with lids, correct the input values for the offending parameter in Plate Lid Definition file when one of these error codes is displayed.
  • Page 105 Offset > Lid Height 2C0B Plate Point Grip Over Long Side Skirt > (Plate Height - Long Side Skirt Height) or < (1.00mm - Long Side Skirt Height)* 2C0C Point Grip Release Gap > 10.00mm 2C0D Plate Clearance > BioTek Instruments, Inc.
  • Page 106 84 | Chapter 6: Troubleshooting & Error Codes Code Description 10.00mm 2C0E Loose Grip Offset > 1.00mm 2C0F Lid Overlap > 10.00mm 2C30 Position Calc - Insufficient claw height for plate & lid at claw position 7* 2C31 Position Calc - Insufficient plate width for gripper in carry position...

  • Page 107: Washer Or Dispenser Communication Error Codes

    The BioStack was requested to send some configuration data to the controlling software, and the BioStack did not know about the requested data. 8302 A checksum was generated following a code download, which did not match what BioTek Instruments, Inc.

  • Page 108: Biostack Sensor Numbers

    & Error Codes Code Probable Cause was expected. Turn the interfacing instrument off/on, and re-try the operation. If the error persists, contact BioTek TAC. 8303 The BioStack is in an invalid mode for receiving a message. Turn the interfacing instrument off/on, check cabling, and re-try the operation.

  • Page 109: Biostack Motor Numbers

    Interfacing Instrument Numbers Instrument Number Any PowerWave, Eon, EpochR; NanoQuot Any Synergy reader except Neo and Neo 2; Cytation 3 and 5 1 ELx405, EL406, MultiFlo/FX, 405 TS/LS MicroFill, MicroFlo Select Reserved for specific customer Precision/XS Custom instrument BioTek Instruments, Inc.

  • Page 110: Plate Stacked Height

    88 | Chapter 6: Troubleshooting & Error Codes Instrument Number Custom instrument Custom instrument Synergy Neo and Synergy Neo2 Plate Stacked Height Not supported in BIOSTACK2WR and when processing plates with lids. Note: Only offered for computer control and touch screen instruments: you must be controlling the BioStack with Gen5, LHC, or a touch screen instrument to take advantage of this option.

  • Page 111: Change Setting For Low-Profile Plates

    Dip Switch Settings on page 28  1. Turn off the BioStack.  2. Two plugs on the rear wall of the instrument cover two sets of dip switches. Remove plug #2, the plug on the right when viewing the instrument from the rear. BioTek Instruments, Inc.

  • Page 112: Loosen Plate Carrier Set Screws - Biostack 4 Only

    : 1/8" Allen (hex) wrench Tools BioStack 4 plate carrier exposed to access 4 set screws  1. Turn off and unplug the BioStack, if necessary.  2. Expose the BioStack's plate carrier: slip your hand inside the front of the BioStack under the gripper, feel for the outer edge of the plate carrier, and gently pull it out.

  • Page 113: Adjust The Stack Dogs

    Communication Error Codes | 91 Adjust the Stack Dogs Caution: This is a delicate procedure, best performed by a technical expert. Consider contacting BioTek TAC to let the professionals resolve your performance problems. Stack Weights may eliminate performance problems! Try using this accessory before adjusting the stack dogs, if possible.
  • Page 114 92 | Chapter 6: Troubleshooting & Error Codes   Adjusting the Stack Dog BioStack™ Microplate Stacker...

  • Page 115: Install The Barcode Scanner

    Appendix A Install the Barcode Scanner This section provides guidelines for installing and using the barcode scanner. Barcode Scanner Overview Install the Barcode Scanner Preparing and Attaching Barcode Labels Scanner Test with Readers Scanner Test with Precision/XS...
  • Page 116 Not compatible with Synergy Neo and Synergy Neo 2, which use their own barcode scanner. Kit Contents BioTek PN 7310017 contains: Barcode scanner assembly: scanner (housed in a protective cover), scanner cable, and mirror attached to a removable mounting bracket...
  • Page 117 BioStack.  3. Using lens tissue or a soft cloth moistened with lens cleaner, carefully wipe the mirror to remove any dust or particles.  4. To complete the installation See Install the Scanner Cable Duct on page 97 BioTek Instruments, Inc.

  • Page 118: Install The Barcode Scanner

    96 | Appendix A: Install the Barcode Scanner Left-Side Installation  1. Using the supplied screwdriver, remove the two screws and washers that attach the scanner assembly to the mounting bracket, as shown below, and set them aside.  2. Using a 9/64" hex wrench, remove the scanner from the bracket. Be very careful not to scratch or damage the mirror.

  • Page 119: Change Dip Switch Setting For Barcode Scanner

    Remove plug #1, the plug on the left when viewing the instrument from the rear.  3. Using a pencil tip, push switch # ) to its position, Switch 1 SW-1-3 Open away from the instrument.  4. Replace the plug. Install the Scanner Cable Duct BioTek Instruments, Inc.
  • Page 120 98 | Appendix A: Install the Barcode Scanner  1. If you have not already done so, turn off and unplug the BioStack.  2. Using a clean, cotton cloth moistened with alcohol, clean the side of the BioStack where the scanner cable duct will be adhered.  3.

  • Page 121: Preparing And Attaching Barcode Labels

    Label position on plate: Attach the labels to the “short” sides of the microplates. Do not let the labels extend above or below the edges of the plate. Overhanging labels can cause the plates stick to one another when they are stacked. BioTek Instruments, Inc.
  • Page 122 100 | Appendix A: Install the Barcode Scanner : the barcode label artwork above may be submitted to a Barcode label artwork print vendor for creating labels that meet the code symbology and industry standards.  Attach the barcode labels to the short side of the microplate. Take into account the position of well A1 and which side of the BioStack the scanner is installed on, to ensure the label will be read.

  • Page 123: Scanner Test With Readers

    In Gen5, open the Data Views dialog (Protocol > Data Views) and highlight Field Group in the tree. Click and enter a Name, such as "Barcode". Click on a cell in the table and then click the button. BioTek Instruments, Inc.
  • Page 124 102 | Appendix A: Install the Barcode Scanner Category Plate Information Field to Barcode. When creating a report or export template, include this data view. BioStack™ Microplate Stacker...

  • Page 125: Scanner Test With Precision/Xs

    The Modifying the To-Do List dialog will open and the three barcode plate maps that you created will appear in the Available Plate Maps list.  7. Select the option to Add to To-Do List via BioStack  8. Configure the Options as follows: BioTek Instruments, Inc.

  • Page 126: To Report Scanned Codes With Precision/Xs

    Correct the problem, place the plate back into the output stack, and home the BioStack. Repeat steps 6 through 9.   If the test does not pass, contact BioTek TAC. To Report Scanned Codes with Precision/XS The BioStack is engaged by a Precision program through a Supply command.
  • Page 127 Index Microplates processing Note: Find instructions for connecting the with lids BioStack to the interfacing instrument in restacking the instrument-specific PDFs provided Minimum requirements with this manual. Packing materials 0 Degree orientation Plate Lid Definition Plate stacked height Plates 10-Plate Stacks loading with pedestal nesting requirements 30-Plate Stacks restacking specifications 9, 45 50-Plate Stacks 6, 30 transfer errors 88-89, 91 Plates with lids 49, 53...

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