User Manuals: Biotek MultiFlo FX Reagent Dispenser

User Manuals: Biotek MultiFlo FX Reagent Dispenser

Manuals and User Guides for Biotek MultiFlo FX Reagent Dispenser. We have 1 Biotek MultiFlo FX Reagent Dispenser manual available for free PDF download: Operator's Manual

Biotek MultiFlo FX Operator's Manual

Biotek MultiFlo FX Operator's Manual (330 pages)

Multi-Mode Dispenser. Microplate Dispenser

Brand: Biotek | Category: Laboratory Equipment | Size: 23 MB

Table of Contents

All Rights Reserved
4
Notices
4
Restrictions and Liabilities
4
Trademarks
4
Table of Contents
5
Global Service and Support
11
Revision History
12
Document Conventions
13
Intended Use Statement
13
Quality Control
13
Dispense Cassette Warranty
14
Repackaging and Shipping
14
Warnings
14
Warranty and Product Registration
14
Hazards and Precautions
15
Introduction
23
Chapter 1 Introduction

24
- Introducing the Multiflo™ FX Dispenser
  24
- Features of the Multiflo FX
  25
  - Washer
    25
  - Peri-Pump Dispenser
    26
  - Syringe Dispensers
    26
  - Liquid Handling Control™ (LHC) Software
    27
  - RAD™ Technology Features
    27
  - Multiflo FX Models
    28
- Multiflo FX Dispenser Comparison
  29
  - Plate Types Table
    31
- Biostack Compatibility
  32
- Package Contents
  34
- Optional Accessories
  35
- Physical Specifications
  40
- Performance Specifications
  43
  - Peri-Pump Dispenser
    43
  - Syringe Dispensers
    44
  - Strip Washer
    45
- Space Requirements
  47
- Biotek's Customer Resource Center
  48
- Installation
  49
Chapter 2 Installation

50
Chapter 3 Operation

114
- Touch Screen Basics
  114
  - Quick Prime
    115
  - Quick Dispense
    116
  - Quick Wash
    117
  - Add Protocols to the Home Screen
    117
  - Remove Protocols from the Home Screen
    117
  - Get Protocols from a Memory Stick - USB Drive
    118
  - Hardware Change Detected
    118
- Optimize Performance
  120
- Create or Modify a Protocol
  124
- Protocol Parameters Tables
  125
- Wash Parameters Table
  130
  - Washer Flow Rates
    133
  - Pre-Dispense
    135
  - Minimum Prime Volumes for the Strip Washer
    136
  - Bottom Wash
    136
  - Change the Plate Type
    137
  - Rename a Protocol
    137
  - Shake/Soak Step Parameters
    138
  - Repeat Steps in a Loop
    138
  - Delay the Protocol
    139
  - Timer Activated Delay
    140
  - RUN: Running Predefined Protocols
    142
  - LHC Users Only: Customize the Predefined Protocols
    142
- Predefined Protocols Listing
  143
- Predefined Sample Protocols
  144
- Operating with the Biostack
  146
  - Use the Biostack
    147
  - Re-Stack Plates in the Biostack
    149
  - Nth Plate to Perform Special Process
    149
  - About the Nth Plate Step
    150
- Advanced Plate Sequencing with the Biostack
  151
- Plate Types and Processing Patterns
  155
- Handling Special Plates and Mini-Tubes
  156
  - 1536F - 1536-Well Flanged Plates
    156
  - Peri-Pump Special Plate Handling
    156
  - Special Plate Carrier for Mini-Tubes
    158
  - Mini-Tube Racks
    159
  - Dispensing to Mini-Tubes
    159
- Dispense Processing Patterns
  160
  - 8-Channel Dispenser Dispense Pattern
    161
  - Dispense Pattern with both Syringes
    162
- Washer Operation
  163
  - About the Multiflo FX Wash Step
    163
- Cell Wash
  164
  - Define a Cell Wash Protocol
    164
  - Cell Wash Strategies
    165
- Biomagnetic Separation - Magnetic Bead Assays
  167
  - Perform Magnetic Bead Assays
    168
  - Optimize Magnetic Bead Protocols
    169
  - Flat Magnets
    169
  - Ring Magnets
    170
  - How to Determine the Magnet Adapter Height Offset
    171
  - Magnet Height Offset
    172
  - Special Procedure for Magnetic Bead Assays
    173
- Peri-Pump Peristaltic Dispenser
  174
- Peri-Pump: How It Works
  175
  - Release the Tension on the Dispense Cassette
    176
- Peri-Pump Dispense Step
  177
  - RAD Tip Tracking
    178
  - RAD Well Map
    179
- Require a Specific Peri-Pump Cassette
  180
- Peri-Pump Advanced Settings
  181
  - Settings for 0.5 Μl Dispense
    181
  - 8-To-1 Tube RAD Cassette
    181
- Dual Syringe Dispenser
  182
  - How to Prime the Syringe Dispenser
    182
- Syringe Prime Step
  183
- Syringe Dispense Step
  185
  - Change the Syringe Dispenser Manifold
    187
- Syringe Dispenser Settings
  188
- Syringe Dispenser- Autoclavable Vs. Non-Autoclavable
  189
- Changing the Instrument's Settings
  190
- Maintenance
  195
Chapter 4 Maintenance

196
- Overview
  196
- Recommended Maintenance Schedule
  198
- Daily Maintenance
  200
- Overnight/Multi-Day Maintenance | 179
  201
- Autoprime
  201
- Soak the Manifold Tubes in Cleaning Fluid
  207
- Removing Protein Residuals and Fungi Growth
  209
- Periodic Maintenance
  211
  - Autoclavable Components
    212
  - Clean the Bottles
    212
  - Clean the Plate Carrier
    213
  - Clean the Exterior Surfaces
    213
- Peri-Pump Dispenser Maintenance
  214
- Syringe Dispenser and Strip Washer Maintenance
  223
  - Clean the Manifolds
    223
  - Clean the Dispense/Aspirate Tubes
    224
  - Clean or Replace the Check Valves
    226
  - Autoclave the Syringe Head
    226
- Decontamination
  227
  - Tools and Supplies
    228
  - Decontaminate Exterior Surfaces
    229
  - Decontaminate Tubing and Manifold
    230
- Long Shutdown (Prepare for Storage or Shipment)
  230
  - Storing the Instrument
    232
- Replace Components
  232
- Calibrate the Backlash for Syringe Dispenser
  234
- Qualification
  237
Chapter 5 Qualification

238
- Qualification Overview
  238
  - Washer
    238
  - Peri-Pump and Syringe Dispensers
    238
- Qualification Schedule
  239
- System Self-Test, Verify Information
  240
  - Record Onboard Software
    240
- Liquid Testing the Multiflo™ FX Multi-Mode Dispenser
  241
  - Which Tests to Perform
    241
  - Important Recommendations for All Liquid Tests
    242
- Peri-Pump Dispense Precision and Accuracy Tests
  243
  - Peri-Pump Dispenser Test Materials
    245
  - Peri-Pump Dispenser Precision and Accuracy Test Solutions
    245
- Perform the Peri-Pump Precision and Accuracy Tests
  246
- Syringe Dispenser Liquid Tests
  257
  - Syringe Dispenser Test Materials
    258
  - Syringe Dispenser Test Solutions
    258
- Perform the Syringe Dispense Precision & Accuracy Test
  258
- Washer Qualification Liquid Tests
  272
  - Dispense Precision and Accuracy Test
    272
  - Evacuation Efficiency Test
    272
  - Washer Qualification Test Materials
    273
  - Create Washer Qualification Protocols
    274
  - Dispense Precision and Accuracy Test
    275
  - Evacuation Efficiency Test
    276
  - Evacuation Diagnostic Test
    278
- Troubleshooting
  293
  - Troubleshooting
    294
    
    Syringe Dispenser Troubleshooting
    295
    
    Dispense Manifold Movement
    295
    
    Fluid Delivery
    296
    
    Fluid Leakage
    298
    
    Microplate Carrier Movement
    298
    
    Syringe Movement
    299
    
    Peri-Pump Troubleshooting
    299
    
    Washer Troubleshooting
    300
    
    Fluid Aspiration
    300
    
    Fluid Delivery
    302
    
    Fluid Leakage
    304
- Error Codes
  305
  - System Error Codes
    306
  - Multiflo FX Software Error Codes
    316
  - Chemical Compatibility
    330

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