Pipette Map - Biotek Synergy HT Operator's Manual

Pipette Map

Seal the plates with foil or store them in black polyethylene bags until use.
If the base of a plate is touched, clean the entire base with alcohol (95%
ethanol) and then wipe with a lint-free cloth. Before placing a plate in the
instrument, blow the bottom of the plate with an aerosol duster.
For the Corners
•
Pipette 200 µL of the
H1-H3, and H10-H12.
•
(Optional for plates with black sidewalls) Pipette 200 µL of buffer in the
wells surrounding the 3.3 nM wells ('CBUF' in the grid below).
For the Sensitivity/Linearity
•
Use a multi-channel pipette with just four tips installed.
•
Pipette 150 µL of buffer into wells C2-F5 (NOT column 1) and C10-F12.
•
Pipette 150 µL of the
•
Pipette 150 µL of the
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C2-F2 and dispense into wells C3-F3.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C3-F3 and dispense into wells C4-F4.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C4-F4 and dispense into wells C5-F5.
Mix the wells using the pipette.
•
Aspirate 150 µL from wells C5-F5 and discard.
1 2
3.3 3.3
A
nM nM
B CBUF CBUF
1.0 0.5
C
nM nM
1.0 0.5
D
nM nM
1.0 0.5
E
nM nM
1.0 0.5
F
nM nM
G CBUF CBUF
3.3 3.3
H
nM nM
Synergy HT Operator's Manual
test:
3.3 nM SF
tests:
1 nM SF
1 nM SF
3 4 5
3.3
CBUF
nM
CBUF CBUF
0.25 0.125 0.062
nM nM nM
0.25 0.125 0.062
nM nM nM
0.25 0.125 0.062
nM nM nM
0.25 0.125 0.062
nM nM nM
CBUF CBUF
3.3
CBUF
nM
Fluorescence Tests |
solution into wells A1-A3, A10-A12,
solution into wells C1-F1. Discard the tips.
solution into wells C2-F2.
6 7 8
CBUF
CBUF
CBUF
CBUF
9 10 11 12
3.3 3.3 3.3
nM nM nM
CBUF CBUF CBUF
BUF BUF BUF
BUF BUF BUF
BUF BUF BUF
BUF BUF BUF
CBUF CBUF CBUF
3.3 3.3 3.3
nM nM nM
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