Biotek Synergy HT Operator's Manual page 7

Multi-mode microplate reader
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Fluorescence Tests ........................................................... 76 
Required Materials ....................................................... 77 
Test Solutions ............................................................. 78 
Procedure .................................................................. 79 
Results Analysis .......................................................... 79 
Troubleshooting .......................................................... 80 
Pipette Map ................................................................ 81 
Gen5 Protocol Reading Parameters ................................ 82 
Dispense Module Tests ..................................................... 86 
Required Materials ....................................................... 87 
Alternate Test Solutions ............................................... 88 
Test Setup ................................................................. 89 
Test Procedure ............................................................ 89 
Results Analysis .......................................................... 91 
Creating the Test Protocols ........................................... 92 
Chapter 5: Preventive Maintenance .................................... 93 
Recommended Maintenance Schedule ................................. 94 
Overview ................................................................... 94 
Daily Cleaning for the Dispense Module .......................... 94 
Recommended Maintenance Schedule ............................ 95 
Warnings & Precautions .................................................... 96 
Cleaning Exposed Surfaces ................................................ 97 
Flush/Purge the Fluid Path ................................................ 99 
Running a Dispense Protocol (Optional) ............................. 100 
Empty/Clean the Tip Priming Trough ................................. 101 
Clean the Priming Plate .................................................... 101 
Clean the Internal Components ......................................... 102 
Required Materials ...................................................... 103 
Removing the Reader's Shroud ..................................... 104 
Cleaning the Optical Probes ......................................... 110 
Cleaning the Reader's Internal Surface .......................... 118 
Reassembling the Components ..................................... 119 
Performance Check ..................................................... 120 
Appendix A: Decontamination .......................................... 123 
Purpose ......................................................................... 124 
Required Materials .......................................................... 125 
Procedure for Models without Injectors .............................. 126 
Routine Procedure for Models with Injectors ....................... 127 
Synergy HT Operator's Manual
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Questions and answers

Luqman Babeer
April 25, 2025

I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you

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1 comments:
Mr. Anderson
May 12, 2025

To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:

1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.

If the issue persists, contact BioTek’s Technical Assistance Center.

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