Biotek Synergy HT Operator's Manual page 76

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Chapter 3: Getting Started
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To keep the dispense system in top condition, flush and purge the fluid lines with
deionized (DI) water every day or upon completion of an assay run, whichever is
more frequent. Some reagents may crystallize or harden after use, clogging the
fluid passageways. Flushing the tubing at the end of each day, letting the DI water
soak them overnight, and then purging the lines at the beginning of each day
ensures optimal performance of the dispense system. See
Maintenance
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For models with injectors:
µL/well, we recommend specifying a tip prime volume that is equal to the
dispense volume. For dispense volumes greater than 20 µL/well, we recommend a
tip prime volume of 20 µL.
for more information.
When dispensing volumes less than or equal to 20
Chapter 5, Preventive
BioTek Instruments, Inc.