Table of Contents
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iv
Preface
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2: Remove the Shipping Panel ........................................... 10
3: Remove the Microplate Carrier Shipping Screw ................. 12
4: Install the Fluorescence Lamp Assembly .......................... 13
5: Select an Appropriate Location ....................................... 14
6: Connect the Power Supply............................................. 14
7: Unpack and Inspect the Dispense Module ........................ 15
8: Install the Dispense Module ........................................... 19
9: Connect the Host Computer .......................................... 23
10: Install Gen5 Software ................................................. 23
11: Turn on the Reader .................................................... 23
12: Establish Communication ............................................ 24
13: Run a System Test ..................................................... 25
14: Test the Injector System ............................................. 26
Operational/Performance Qualification ................................ 28
Repackaging and Shipping Instructions ............................... 28
Chapter 3: Getting Started ................................................. 33
Key Components ............................................................. 34
Lamp Assembly and Filter Wheel Access ......................... 35
Excitation and Emission Filter Wheels ............................. 36
The External Dispense Module ....................................... 41
Gen5 Software ................................................................ 44
Creating Protocols and Experiments ............................... 45
Controlling the Dispense Module .................................... 49
Recommendations for Optimum Performance ....................... 51
Chapter 4: Instrument Qualification ................................... 53
Overview ........................................................................ 54
IQ/OQ/PQ ....................................................................... 54
Recommended Qualification Schedule ................................. 56
System Test .................................................................... 57
Absorbance Plate Test ...................................................... 62
Test Plate Certificates .................................................. 62
Define Absorbance Test Plate Parameters ....................... 62
Run the Absorbance Plate Test ...................................... 63
Results and Troubleshooting Tips ................................... 66
Absorbance Liquid Tests ................................................... 68
Absorbance Liquid Test 1 .............................................. 69
Absorbance Liquid Test 2 .............................................. 71
Absorbance Liquid Test 3 (optional) ............................... 74
BioTek Instruments, Inc.
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Table of Contents
Troubleshooting
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Do you have a question about the Synergy HT and is the answer not in the manual?
Questions and answers
I Connected ELx800 with Gen5 3.12 .When i check the connection it is succeeded because i was make plate in and plate out successfully and message appear that connected successfully .But when i need to run plate or tests the message appear (Reader Failed to acknowledge request) or when i need to make validation it will be fail . I don't know how can I resolve this issue. I sent the errors photo here Thank you
To troubleshoot the "Reader Failed to acknowledge request" error on the Biotek Synergy HT when connected to ELx800 with Gen5 3.12, follow these steps:
1. Ensure the reader is connected to the power supply and turned on.
2. Verify the communication cable is firmly attached to both the reader and the computer.
3. Confirm the correct Reader Type is selected in Gen5.
4. Try using a different COM port.
5. If using a USB cable, ensure the driver software is installed.
If the issue persists, contact BioTek’s Technical Assistance Center.
This answer is automatically generated