Controlling The Dispense Module - Biotek Synergy HT Operator's Manual

Controlling the Dispense Module

Applies only to Synergy HT models with injectors.
Gen5 is used to perform several dispense module-specific functions, including
initializing, priming, and purging. Gen5 also contains certain configuration items that
must be set before using the dispense module. Read the following sections to become
familiar with these functions and configuration items.
Initialization
If the dispense module was connected to the reader before the reader was turned
on, or if a System Test was run via Gen5, the dispense module should initialize
automatically. If for any reason the module does not initialize automatically, you
can initialize it from Gen5:
1. In Gen5, select
(Com<#>)
2. Select the desired
button. The syringe drive will move to its home position and its sensors will
be verified. Upon successful completion, the
"Yes".
Prime Utility
Before running an experiment with a Dispense step, the dispense module and its
associated tubing must be primed with the fluid to be used. Gen5 provides a
special utility for this task. To prime the dispense module:
1. Fill the supply bottle with a sufficient volume of the fluid to be used for the
prime and the assay. Insert the appropriate inlet tube into the bottle.
2. Important!
3. In Gen5, select
(Com<#>)
4. Select the
5. Enter the
The minimum recommended prime volume is 1100 µL.
6. Select a prime
7. Click
8. When the process is complete, carefully remove the priming plate from the
carrier and empty its contents. If the priming plate is empty, the prime
volume was too low.
Synergy HT Operator's Manual
System > Reader/Instrument Control > Synergy HT
and click the Dispenser
Dispenser
Place the priming plate on the carrier.
System > Reader/Instrument Control > Synergy HT
and click the Dispenser
Dispenser number (1 or 2) associated with the supply bottle.
Volume to be used for the prime, from 5 to 5000 µL.
Rate , in µL/second.
Prime to start the process.
tab.
number (1 or 2) and click the
Initialized
tab.
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Gen5 Software |
Initialize
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