Troubleshooting - Biotek Synergy HT Operator's Manual

80
Chapter 4: Instrument Qualification
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Linearity Test
1. Calculate the Mean of the four wells for each concentration in columns 1-5 (rows
C-F only).
2. Perform linear regression using these values as inputs:
1000
500
250
125
62.5
3. Calculate the R-Squared value; it must be
pass.

Troubleshooting

If any tests fail, please try the suggestions below. If the test(s) continue to fail, print the
results and contact BioTek's Technical Assistance Center.
•
Are the solutions fresh? The open buffer and stock solutions should be
discarded after seven days.
•
Are the Excitation/Emission filters clean? Are they in the proper locations and
in the proper orientation in the filter wheels?
•
Are you using new/clean plates? We suggest you re-run the test with a
new/clean microplate. For the bottom optics test, if the base of a plate is
touched, clean the entire base with alcohol (95% ethanol) and then wipe with a
lint-free cloth. Before placing a plate in the instrument, blow the bottom of the
plate with an aerosol. If the test fails again, the optical probe(s) may need to be
cleaned. Contact BioTek TAC for instructions.
•
If a test fails because one or more wells overranged, reduce the Gain
(Sensitivity) value in the protocol by 1-5 counts and re-read the plate.
•
If the Corners Test continues to fail, the hardware may be misaligned. Contact
BioTek TAC.
•
Review the instructions under "Pipette Map" (page 81 for SF or 85 for MUB) to
verify that you correctly prepared the plates.
•
Does the Plate Type setting in the protocol match the plate you used?
x
mean of the 1000 pM (1.0 nM) wells
mean of the 500 pM (0.5 nM) wells
mean of the 250 pM (0.25 nM) wells
mean of the 125 pM (0.125 nM) wells
mean of the 62.5 pM (0.0625 nM) wells
y
greater than or equal to 0.950 to
BioTek Instruments, Inc.